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Merhof, Dorit

Biomedical Engineering / Biomedizinische Technik

Joint Journal of the German Society for Biomedical Engineering in VDE and the Austrian and Swiss Societies for Biomedical Engineering and the German Society of Biomaterials

Editor-in-Chief: Dössel, Olaf

Editorial Board: Augat, Peter / Habibović, Pamela / Haueisen, Jens / Jahnen-Dechent, Wilhelm / Jockenhoevel, Stefan / Knaup-Gregori, Petra / Leonhardt, Steffen / Plank, Gernot / Radermacher, Klaus M. / Schkommodau, Erik / Stieglitz, Thomas / Boenick, Ulrich / Jaramaz, Branislav / Kraft, Marc / Lenarz, Thomas / Lenthe, Harry / Lo, Benny / Mainardi, Luca / Micera, Silvestro / Penzel, Thomas / Robitzki, Andrea A. / Schaeffter, Tobias / Snedeker, Jess G. / Sörnmo, Leif / Sugano, Nobuhiko / Werner, Jürgen /

IMPACT FACTOR 2018: 1.007
5-year IMPACT FACTOR: 1.390

CiteScore 2018: 1.24

SCImago Journal Rank (SJR) 2018: 0.282
Source Normalized Impact per Paper (SNIP) 2018: 0.831

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Volume 61, Issue 5


Volume 57 (2012)

Development of antibiotic-loaded silk fibroin/hyaluronic acid polyelectrolyte film coated CoCrMo alloy

Pınar Arpaçay
  • Nanotechnology Graduate Program, Gediz University, Seyrekköy, Menemen, 35200 Izmir, Turkey
  • CRANN and AMBER Research Centers, Trinity College Dublin, Dublin 2, Ireland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Uğur Türkan
Published Online: 2015-11-05 | DOI: https://doi.org/10.1515/bmt-2015-0061


Bacteria related infections are still a major problem for the implant materials. Such infections have occurred in nearly 3% of hip and knee replacements resulting in failure of device. There are two main approaches for inhibiting the bacterial adhesion to the surface. These involve bactericidal substances and anti-adhesive coatings. In this study, the efficiency of antibiotic-loaded silk fibroin/hyaluronic acid polyelectrolyte film coated CoCrMo alloy, prepared by means of complex coacervate and layer by layer techniques, was investigated. A medical grade CoCrMo was coated with variable number of silk fibroin/hyaluronic acid up to 14 layers at room temperature. The morphological evolution during and after formation of the crystal structure on the coating layer, the resulting surface roughness, and the corresponding alterations in the coating layer thicknesses were thoroughly studied using various analytical techniques, including attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM) and atomic force microscopy (AFM). As a result, only 10 layers of silk fibroin/hyaluronic acid complex coacervate films were found to convey the general characteristics of the mixture of silk I and II, while layer by layer coated samples exhibited the mixture of silk I and II. Moreover, regardless of the preparation method applied, the surface roughness and the coating layer thicknesses were determined to increase with the increasing number of layers. The antibacterial test results suggested that the samples loaded with antibiotic successfully induced a bactericidal resistance against Staphylococcus aureus bacteria.

Keywords: antibiotic; complex coacervate; hyaluronic acid; LbL; S. aureus; silk fibroin


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About the article

Corresponding author: Uğur Türkan, Department of Biomedical Engineering, Gediz University, Seyrekköy, Menmen, İzmir, Turkey, Phone: +90 (232) 3550000/2318, Fax: +90 (232) 3550024, E-mail:

Received: 2015-03-22

Accepted: 2015-09-30

Published Online: 2015-11-05

Published in Print: 2016-10-01

Citation Information: Biomedical Engineering / Biomedizinische Technik, Volume 61, Issue 5, Pages 463–474, ISSN (Online) 1862-278X, ISSN (Print) 0013-5585, DOI: https://doi.org/10.1515/bmt-2015-0061.

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