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Merhof, Dorit

Biomedical Engineering / Biomedizinische Technik

Joint Journal of the German Society for Biomedical Engineering in VDE and the Austrian and Swiss Societies for Biomedical Engineering and the German Society of Biomaterials

Editor-in-Chief: Dössel, Olaf

Editorial Board: Augat, Peter / Habibović, Pamela / Haueisen, Jens / Jahnen-Dechent, Wilhelm / Jockenhoevel, Stefan / Knaup-Gregori, Petra / Leonhardt, Steffen / Plank, Gernot / Radermacher, Klaus M. / Schkommodau, Erik / Stieglitz, Thomas / Boenick, Ulrich / Jaramaz, Branislav / Kraft, Marc / Lenarz, Thomas / Lenthe, Harry / Lo, Benny / Mainardi, Luca / Micera, Silvestro / Penzel, Thomas / Robitzki, Andrea A. / Schaeffter, Tobias / Snedeker, Jess G. / Sörnmo, Leif / Sugano, Nobuhiko / Werner, Jürgen /

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Volume 62, Issue 1


Volume 57 (2012)

Different performances of CXCR4, integrin-1β and CCR-2 in bone marrow stromal cells (BMSCs) migration by low-intensity pulsed ultrasound stimulation

Weixiong Xiao / Qian Xu / Zhimin Zhu / Lei Li / Wenchuan Chen
  • Corresponding author
  • West China Hospital of Stomatology, Sichuan University, NO. 14, 3 Section of Ren Min Nan Rd. Chengdu, Sichuan 610041, China
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Published Online: 2016-04-23 | DOI: https://doi.org/10.1515/bmt-2015-0166


Low-intensity pulsed ultrasound (LIPUS) is an established therapy for fracture healing where bone marrow stromal cells (BMSCs) migration is crucial to bone regeneration. This work focused on different performances of C-X-C-receptor 4 (CXCR4), integrin-1β and chemokine-chemokine receptor2 (CCR-2) in BMSCs migration by LIPUS stimulation. Single 20-min LIPUS treatment was applied to BMSCs during wound healing assay with or without the inhibitor AMD3100. The migration rate of BMSCs with LIPUS stimulation exhibited a higher closure rate than that of BMSCs without LIPUS stimulation, which was 1.89 μm/h and 1.38 μm/h, respectively. After LIPUS stimulation, significant elevation of the expression of CXCR4, integrin-1β and CCR-2 was observed. When AMD3100 was added, the migration rate of the BMSCs was obviously declined with or without LIPUS treatment. Furthermore, the expression of CXCR4 was significantly down-regulated by AMD3100, while integrin-1β and CCR-2 were less affected. It suggested that the enhancement of the migration of the BMSCs by LIPUS was inhibited by AMD3100. The results confirmed that LIPUS stimulation was able to activate and improve migration of BMSCs. Nevertheless, CXCR4 and both integrin-1β and CCR-2 had different roles in BMSCs migration after LIPUS treatment.

Keywords: bone marrow stromal cells; low-intensity pulsed ultrasound; migration; AMD3100; CXCR4


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About the article

Corresponding author: Dr. Wenchuan Chen, Associate Professor, West China Hospital of Stomatology, Sichuan University, NO. 14, 3rd Section of Ren Min Nan Rd. Chengdu, Sichuan 610041, China

aWeixiong Xiao and Qian Xu: These authors contributed equally to this work.

Received: 2015-08-26

Accepted: 2016-03-24

Published Online: 2016-04-23

Published in Print: 2017-02-01

Funding Source: National Natural Science Foundation of China

Award identifier / Grant number: 81000455

The authors gratefully thank Dr. Lijun Tan and Prof. Yunfeng Lin, for providing the ultrasound transducer. This study was supported by a project of National Natural Science Foundation of China (Grant No. 81000455), and by State Key Laboratory of Oral Diseases at the West China School of Stomatology, Sichuan University.

Citation Information: Biomedical Engineering / Biomedizinische Technik, Volume 62, Issue 1, Pages 89–95, ISSN (Online) 1862-278X, ISSN (Print) 0013-5585, DOI: https://doi.org/10.1515/bmt-2015-0166.

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