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Merhof, Dorit

Biomedical Engineering / Biomedizinische Technik

Joint Journal of the German Society for Biomedical Engineering in VDE and the Austrian and Swiss Societies for Biomedical Engineering and the German Society of Biomaterials

Editor-in-Chief: Dössel, Olaf

Editorial Board: Augat, Peter / Habibović, Pamela / Haueisen, Jens / Jahnen-Dechent, Wilhelm / Jockenhoevel, Stefan / Knaup-Gregori, Petra / Leonhardt, Steffen / Plank, Gernot / Radermacher, Klaus M. / Schkommodau, Erik / Stieglitz, Thomas / Boenick, Ulrich / Jaramaz, Branislav / Kraft, Marc / Lenarz, Thomas / Lenthe, Harry / Lo, Benny / Mainardi, Luca / Micera, Silvestro / Penzel, Thomas / Robitzki, Andrea A. / Schaeffter, Tobias / Snedeker, Jess G. / Sörnmo, Leif / Sugano, Nobuhiko / Werner, Jürgen /

IMPACT FACTOR 2018: 1.007
5-year IMPACT FACTOR: 1.390

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Volume 62, Issue 1


Volume 57 (2012)

Analysis of compound action potentials elicited with specific current stimulating pulses in an isolated rat sciatic nerve

Monika C. Žužek
  • Institute of Physiology, Pharmacology and Toxicology, Veterinary Faculty, University of Ljubljana, 1000 Ljubljana, Slovenia
  • Other articles by this author:
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/ Janez Rozman
  • Center for Implantable Technology and Sensors, ITIS d. o. o. Ljubljana, 1000 Ljubljana, Slovenia
  • Institute of Pathophysiology, Medical Faculty, University of Ljubljana, 1000 Ljubljana, Slovenia
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Polona Pečlin
  • Center for Implantable Technology and Sensors, ITIS d. o. o. Ljubljana, 1000 Ljubljana, Slovenia
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Milka Vrecl
  • Institute of Anatomy, Histology and Embryology, Veterinary Faculty, University of Ljubljana, 1000 Ljubljana, Slovenia
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Robert Frangež
  • Corresponding author
  • Institute of Physiology, Pharmacology and Toxicology, Veterinary Faculty, University of Ljubljana, 1000 Ljubljana, Slovenia
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2016-03-04 | DOI: https://doi.org/10.1515/bmt-2015-0167


The ability to selectively stimulate Aα, Aβ-fibers and Aδ-fibers in an isolated rat sciatic nerve (SNR) was assessed. The stimulus used was a current, biphasic pulse with a quasitrapezoidal cathodic phase and rectangular anodic phase where parameters were systematically varied: intensity of the cathodic phase (ic); width of the cathodic phase (tc); width of the cathodic exponential decay (texp) and time constant of the exponential decay (τexp). A SNR was stimulated using a pair of hook electrodes while conduction velocity (CV) and compound action potentials (CAP) were measured at two sites along the SNR using another two pairs of electrodes. Results showed that the highest CAP1 (8.5–9 mV), shall be expected when parameters of the stimulus were within the following range: ic=3.8–4 mA, tc=350–400 μs and texp=330–440 μs. Results also showed that with ascending tc and texp, CV of the corresponding superficial region of the SNR was reduced in both, conduction velocity of CAP1 and conduction velocity of CAP2. It was concluded that action potentials (APs) were activated in the Aβ-fibers and Aδ-fibers along with a slight AP inhibition in the Aβ-fibers. The obtained results, could serve as a tool for developing multi-electrode systems that potentially enable fiber-type selective stimulation of nerve fibers.

Keywords: compound action potential; excitability; “in vitro setup”; nerve conduction velocity; nerve fiber diameter; neural prosthesis; rat sciatic nerve; selective nerve stimulation


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About the article

Corresponding author: Prof. Dr. Robert Frangež, Institute of Physiology, Pharmacology and Toxicology, Veterinary Faculty, University of Ljubljana, Gerbičeva 60, 1000 Ljubljana, Slovenia, Phone: +386 31 782 356, E-mail:

Received: 2015-08-27

Accepted: 2016-01-26

Published Online: 2016-03-04

Published in Print: 2017-02-01

Ethical approval: The neural tissue was treated in accordance with the approval guidelines of the Ethics Committee of the Veterinary Administration, Ministry of Agriculture, Forestry and Food, Republic of Slovenia (permit no. 34401-12/2012/2).

Conflict of interest statement: The authors state that there are no conflicts of interest related to the results presented within the paper. None of the authors received any financial or other compensation for undertaking this study or during its execution. No personal relationships with other people or organizations inappropriately influenced the work.

Citation Information: Biomedical Engineering / Biomedizinische Technik, Volume 62, Issue 1, Pages 37–48, ISSN (Online) 1862-278X, ISSN (Print) 0013-5585, DOI: https://doi.org/10.1515/bmt-2015-0167.

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