Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Biomedical Engineering / Biomedizinische Technik

Joint Journal of the German Society for Biomedical Engineering in VDE and the Austrian and Swiss Societies for Biomedical Engineering and the German Society of Biomaterials

Editor-in-Chief: Dössel, Olaf

Editorial Board: Augat, Peter / Habibović, Pamela / Haueisen, Jens / Jahnen-Dechent, Wilhelm / Jockenhoevel, Stefan / Knaup-Gregori, Petra / Lenarz, Thomas / Leonhardt, Steffen / Plank, Gernot / Radermacher, Klaus M. / Schkommodau, Erik / Stieglitz, Thomas / Boenick, Ulrich / Jaramaz, Branislav / Kraft, Marc / Lenthe, Harry / Lo, Benny / Mainardi, Luca / Micera, Silvestro / Penzel, Thomas / Robitzki, Andrea A. / Schaeffter, Tobias / Snedeker, Jess G. / Sörnmo, Leif / Sugano, Nobuhiko / Werner, Jürgen /


IMPACT FACTOR 2017: 1.096
5-year IMPACT FACTOR: 1.492

CiteScore 2017: 0.48

SCImago Journal Rank (SJR) 2017: 0.202
Source Normalized Impact per Paper (SNIP) 2017: 0.356

Online
ISSN
1862-278X
See all formats and pricing
More options …
Volume 62, Issue 5

Issues

Volume 57 (2012)

Umbilical cord as human cell source for mitral valve tissue engineering – venous vs. arterial cells

Axel Malischewski
  • Institute of Applied Medical Engineering, Helmholtz Institute, RWTH Aachen University, 52074 Aachen, Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Ricardo Moreira
  • Institute of Applied Medical Engineering, Helmholtz Institute, RWTH Aachen University, 52074 Aachen, Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Luis Hurtado
  • Institute of Applied Medical Engineering, Helmholtz Institute, RWTH Aachen University, 52074 Aachen, Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Valentine Gesché / Thomas Schmitz-Rode
  • Institute of Applied Medical Engineering, Helmholtz Institute, RWTH Aachen University, 52074 Aachen, Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Stefan Jockenhoevel
  • Corresponding author
  • Institut für Textiltechnik, RWTH Aachen University, 52074 Aachen, Germany
  • Department of Biohybrid and Medical Textiles, AME-Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, Pauwelsstr. 20, 52074 Aachen, Germany
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Petra Mela
  • Corresponding author
  • Department of Biohybrid and Medical Textiles, AME-Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, Pauwelsstr. 20, 52074 Aachen, Germany
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2017-04-28 | DOI: https://doi.org/10.1515/bmt-2016-0218

Abstract

Around 2% of the population in developed nations are affected by mitral valve disease and available valvular replacements are not designed for the atrioventricular position. Recently our group developed the first tissue-engineered heart valve (TEHV) specifically designed for the mitral position – the TexMi valve. The valve recapitulates the main components of the native valve, i.e. annulus, asymmetric leaflets and the crucial chordae tendineae. In the present study, we evaluated the human umbilical cord as a clinically applicable cell source for the TexMi valve. Valves produced with cells isolated from human umbilical cord veins (HUVs) and human umbilical cord arteries (HUAs) were conditioned for 21 days in custom-made bioreactors and evaluated in terms of extracellular matrix (ECM) composition and mechanical properties. In addition, static cell-laden fibrin discs were molded to investigate cell-mediated tissue contraction and differences in ECM production. HUA and HUV cells were able to deliver functional valves with a rich ECM composed mainly of collagen. Particularly noteworthy was the synthesis of elastin, which has been observed rarely in TEHV. The elastin synthesis was significantly higher in TexMi valves produced with HUV cells and therefore the HUV is considered to be the preferred cell source.

Keywords: elastin; elastogenesis; extracellular matrix; fibrin; scaffold

References

  • [1]

    El Oakley R, Kleine P, Bach DS. Choice of prosthetic heart valve in today’s practice. Circulation 2008; 117: 253.CrossrefPubMedWeb of ScienceGoogle Scholar

  • [2]

    Enriquez-Sarano M, Akins CW, Vahanian A. Mitral regurgitation. Lancet 2009; 373: 1382.Web of SciencePubMedCrossrefGoogle Scholar

  • [3]

    Flanagan TC, Cornelissen C, Koch S, et al. The in vitro development of autologous fibrin-based tissue-engineered heart valves through optimised dynamic conditioning. Biomaterials 2007; 28: 3388.PubMedCrossrefWeb of ScienceGoogle Scholar

  • [4]

    Flanagan TC, Sachweh JS, Frese J, et al. In vivo remodeling and structural characterization of fibrin-based tissue-engineered heart valves in the adult sheep model. Tissue Eng Part A 2009; 15: 2965.PubMedCrossrefWeb of ScienceGoogle Scholar

  • [5]

    Gauvin R, Guillemette M, Galbraith T, et al. Mechanical properties of tissue-engineered vascular constructs produced using arterial or venous cells. Tissue Eng Part A 2011; 17: 2049.PubMedWeb of ScienceCrossrefGoogle Scholar

  • [6]

    Gupta V, Grande-Allen KJ. Effects of static and cyclic loading in regulating extracellular matrix synthesis by cardiovascular cells. Cardiovasc Res 2006; 72: 375.PubMedCrossrefGoogle Scholar

  • [7]

    Johnson DJ, Robson P, Hew Y, Keeley FW. Decreased elastin synthesis in normal development and in long-term aortic organ and cell cultures is related to rapid and selective destabilization of mRNA for elastin. Circ Res 1995; 77: 1107.PubMedCrossrefGoogle Scholar

  • [8]

    Kadner A, Zund G, Maurus C, et al. Human umbilical cord cells for cardiovascular tissue engineering: a comparative study. Eur J Cardiothorac Surg 2004; 25: 635.CrossrefPubMedGoogle Scholar

  • [9]

    Kielty CM, Baldock C, Lee D, Rock MJ, Ashworth JL, Shuttleworth CA. Fibrillin: from microfibril assembly to biomechanical function. Philos Trans R Soc Lond B Biol Sci 2002; 357: 207.CrossrefPubMedGoogle Scholar

  • [10]

    Koch S, Stappenbeck N, Cornelissen CG, et al. Tissue engineering: selecting the optimal fixative for immunohistochemistry. Tissue Eng Part C Methods 2012; 18: 976.Web of SciencePubMedCrossrefGoogle Scholar

  • [11]

    Lee TC, Midura RJ, Hascall VC, Vesely I. The effect of elastin damage on the mechanics of the aortic valve. J Biomech 2001; 34: 203.CrossrefPubMedGoogle Scholar

  • [12]

    Lee KW, Stolz DB, Wang Y. Substantial expression of mature elastin in arterial constructs. Proc Natl Acad Sci USA 2011; 108: 2705.CrossrefGoogle Scholar

  • [13]

    Long JL, Tranquillo RT. Elastic fiber production in cardiovascular tissue-equivalents. Matrix Biology 2003; 22: 339.CrossrefGoogle Scholar

  • [14]

    McDonald PC, Wilson JE, McNeill S, et al. The challenge of defining normality for human mitral and aortic valves: geometrical and compositional analysis. Cardiovasc Pathol 2002; 11: 193.CrossrefPubMedGoogle Scholar

  • [15]

    McMahon MP, Faris B, Wolfe BL, et al. Aging effects on the elastin composition in the extracellular matrix of cultured rat aortic smooth muscle cells. In Vitro Cell Dev Biol 1985; 21: 674.PubMedCrossrefGoogle Scholar

  • [16]

    Mol A, Smits AI, Bouten CV, Baaijens FP. Tissue engineering of heart valves: advances and current challenges. Expert Rev Med Devices 2009; 6: 259.CrossrefWeb of SciencePubMedGoogle Scholar

  • [17]

    Moreira R, Gesche VN, Hurtado-Aguilar LG, et al. TexMi: development of tissue-engineered textile-reinforced mitral valve prosthesis. Tissue Eng Part C Methods 2014; 20: 741.CrossrefWeb of SciencePubMedGoogle Scholar

  • [18]

    Moreira R, Velz T, Alves N, et al. Tissue-engineered heart valve with a tubular leaflet design for minimally invasive transcatheter implantation. Tissue Eng Part C Methods 2015; 21: 530–540.Web of ScienceCrossrefPubMedGoogle Scholar

  • [19]

    Reardon MJ, David TE. Mitral valve replacement with preservation of the subvalvular apparatus. Curr Opin Cardiol 1999; 14: 104.PubMedCrossrefGoogle Scholar

  • [20]

    Reddy GK, Enwemeka CS. A simplified method for the analysis of hydroxyproline in biological tissues. Clin Biochem 1996; 29: 225.CrossrefPubMedGoogle Scholar

  • [21]

    Reul H, Talukder N, Muller EW. Fluid mechanics of the natural mitral valve. J Biomech 1981; 14: 361.PubMedCrossrefGoogle Scholar

  • [22]

    Sodian R, Lueders C, Kraemer L, et al. Tissue engineering of autologous human heart valves using cryopreserved vascular umbilical cord cells. Ann Thorac Surg 2006; 81: 2207.CrossrefPubMedGoogle Scholar

  • [23]

    Sutcliffe MC, Davidson JM. Effect of static stretching on elastin production by porcine aortic smooth muscle cells. Matrix 1990; 10: 148.PubMedCrossrefGoogle Scholar

  • [24]

    Syedain ZH. Implantation of a tissue-engineered heart valve from human fibroblasts exhibiting short term function in the sheep pulmonary artery. Cardiovasc Eng Technol 2010; 2: 101.Google Scholar

  • [25]

    Syedain ZH, Weinberg JS, Tranquillo RT. Cyclic distension of fibrin-based tissue constructs: evidence of adaptation during growth of engineered connective tissue. Proc Natl Acad Sci USA 2008; 105: 6537.CrossrefGoogle Scholar

  • [26]

    Turrentine MW, Ruzmetov M, Vijay P, Bills RG, Brown JW. Biological versus mechanical aortic valve replacement in children. Ann Thorac Surg 2001; 71: S356.PubMedCrossrefGoogle Scholar

  • [27]

    Weber M, Heta E, Moreira R, et al. Tissue-engineered fibrin-based heart valve with a tubular leaflet design. Tissue Eng Part C Methods 2014; 20: 265.CrossrefWeb of SciencePubMedGoogle Scholar

About the article

Corresponding authors: Univ.-Prof. Dr. med. Stefan Jockenhoevel, Department of Biohybrid and Medical Textiles, AME-Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, Pauwelsstr. 20, 52074 Aachen, Germany, Phone/Fax: +49 (0)241-80-85640/-82442 and Petra Mela, PhD, Department of Biohybrid and Medical Textiles, AME-Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, Pauwelsstr. 20, 52074 Aachen, Germany, Phone/Fax: +49 (0)241-80-85640/-82442


Received: 2016-11-12

Accepted: 2017-03-01

Published Online: 2017-04-28

Published in Print: 2017-10-26


Conflict of interest statement: No competing financial interests exist.


Citation Information: Biomedical Engineering / Biomedizinische Technik, Volume 62, Issue 5, Pages 457–466, ISSN (Online) 1862-278X, ISSN (Print) 0013-5585, DOI: https://doi.org/10.1515/bmt-2016-0218.

Export Citation

©2017 Walter de Gruyter GmbH, Berlin/Boston.Get Permission

Comments (0)

Please log in or register to comment.
Log in