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Biomedical Engineering / Biomedizinische Technik

Joint Journal of the German Society for Biomedical Engineering in VDE and the Austrian and Swiss Societies for Biomedical Engineering and the German Society of Biomaterials

Editor-in-Chief: Dössel, Olaf

Editorial Board: Augat, Peter / Habibović, Pamela / Haueisen, Jens / Jahnen-Dechent, Wilhelm / Jockenhoevel, Stefan / Knaup-Gregori, Petra / Lenarz, Thomas / Leonhardt, Steffen / Plank, Gernot / Radermacher, Klaus M. / Schkommodau, Erik / Stieglitz, Thomas / Boenick, Ulrich / Jaramaz, Branislav / Kraft, Marc / Lenthe, Harry / Lo, Benny / Mainardi, Luca / Micera, Silvestro / Penzel, Thomas / Robitzki, Andrea A. / Schaeffter, Tobias / Snedeker, Jess G. / Sörnmo, Leif / Sugano, Nobuhiko / Werner, Jürgen /

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Volume 63, Issue 5


Volume 57 (2012)

A compact hyperspectral camera for measurement of perfusion parameters in medicine

Axel Kulcke / Amadeus Holmer / Philip Wahl / Frank Siemers
  • Department of Plastic and Hand Surgery and Burn Unit, BG Klinikum Bergmannstrost, Merseburger Strasse 165, 06002 Halle (Saale), Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Thomas Wild
  • Department of Plastic, Aesthetic and Hand Surgery, Interdisciplinary Center for Treatment of Chronic Wounds, Auenweg 38, 06847 Dessau, Germany
  • Department of Dermatology, Venereology, Allergology and Immunology, Dessau Medical Center, Brandenburg Medical School Theodore Fontane, Auenweg 38, 06847 Dessau, Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Georg Daeschlein
  • Department of Dermatology, University Medicine Greifswald, Sauerbruchstr., 17475 Greifswald, Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2018-03-09 | DOI: https://doi.org/10.1515/bmt-2017-0145


Worldwide, chronic wounds are still a major and increasing problem area in medicine with protracted suffering of patients and enormous costs. Beside conventional wound treatment, for instance kinds of oxygen therapy and cold plasma technology have been tested, providing an improvement in the perfusion of wounds and their healing potential, but these methods are unfortunately not sufficiently validated and accepted for clinical practice to date. Using hyperspectral imaging technology in the visible (VIS) and near infrared (NIR) region with high spectral and spatial resolution, perfusion parameters of tissue and wounds can be determined. We present a new compact hyperspectral camera which can be used in clinical practice. From hyperspectral data the hemoglobin oxygenation (StO2), the relative concentration of hemoglobin [tissue hemoglobin index (THI)] and the so-called NIR-perfusion index can be determined. The first two parameters are calculated from the VIS-part of the spectrum and represent the perfusion of superficial tissue layers, whereas the NIR-perfusion index is calculated from the NIR-part representing the perfusion in deeper layers. First clinical measurements of transplanted flaps and chronic ulcer wounds show, that the perfusion level can be determined quantitatively allowing sensitive evaluation and monitoring for an optimization of the wound treatment planning and for validation of new treatment methods.

Keywords: free flap monitoring; hyperspectral imaging; perfusion imaging; tissue oxygenation; wound diagnostics


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About the article

Received: 2017-08-18

Accepted: 2017-12-04

Published Online: 2018-03-09

Published in Print: 2018-10-25

Author Statement

Research funding: Authors state no funding involved.

Conflict of interest: The hyperspectral camera prototype described in this publication was developed by Diaspective Vision GmbH. The first, second and third author are employees of this company. In the long term, Diaspective Vision has proprietary interest in the development of the camera system resulting in a product for routine clinical use. The clinical tests of the camera have been performed by clinicians (authors 4, 5 and 6). We certify that the clinical investigators and co-authors have no financial interests and financial arrangements with Diaspective Vision and have received no funding for the measurements and/or preparation of this manuscript. The cameras used during the measurements have been provided by Diaspective Vision.

Informed consent: All patients have signed an informed consent.

Ethical approval: Experimental hyperspectral measurements from patients for the evaluation of the new technology for perfusion measurements and wound description have obtained the ethics approval by the Ethics committee of the Ärztekammer Sachsen-Anhalt, Germany (35/17) and the Ethics Committee of the University of Greifswald (EUDAMED-No. CIV-17-02-018504). The study was conducted according to the Declaration of Helsinki.

Citation Information: Biomedical Engineering / Biomedizinische Technik, Volume 63, Issue 5, Pages 519–527, ISSN (Online) 1862-278X, ISSN (Print) 0013-5585, DOI: https://doi.org/10.1515/bmt-2017-0145.

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