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Biomedical Engineering / Biomedizinische Technik

Editor-in-Chief: Dössel, Olaf

Editorial Board Member: Augat, Peter / Haueisen, Jens / Jockenhoevel, Stefan / Lenarz, Thomas / Leonhardt, Steffen / Plank, Gernot / Radermacher, Klaus M. / Schkommodau, Erik / Schmitz, Georg / Stieglitz, Thomas / Witte, Herbert / Boenick, Ulrich / Jaramaz, Branislav / Kraft, Marc / Lenthe, Harry / Lo, Benny / Mainardi, Luca / Micera, Silvestro / Penzel, Thomas / Robitzki, Andrea A. / Schaeffter, Tobias / Snedeker, Jess G. / Sörnmo, Leif / Sugano, Nobuhiko / Werner, Jürgen / Wintermantel, Erich /

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Volume 57 (2012)

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In situ optical coherence tomography of percutaneous implant-tissue interfaces in a murine model

Sabine Donner
  • Biomedical Optics Department, Laser Zentrum Hannover e.V., D-30419 Hannover, Germany
  • CrossBIT, Hannover Medical School, 30625 Hannover, Germany
  • :
/ Oliver Müller
  • Institute for Information Processing, Leibniz University of Hannover, D-30167 Hannover, Germany
/ Frank Witte
  • Department of Orthopaedics/CrossBIT, Hannover Medical School, 30625 Hannover, Germany
/ Ivonne Bartsch
  • Department of Orthopaedics/CrossBIT, Hannover Medical School, 30625 Hannover, Germany
  • Currently affiliated with the Department of Experimental Otology, Institute of Audioneurotechnology, Hannover Medical School, D-30625 Hannover, Germany.
/ Elmar Willbold
  • Department of Orthopaedics/CrossBIT, Hannover Medical School, 30625 Hannover, Germany
/ Tammo Ripken
  • Biomedical Optics Department, Laser Zentrum Hannover e.V., D-30419 Hannover, Germany
/ Alexander Heisterkamp
  • Institute of Applied Optics, Friedrich-Schiller-University Jena, D-07743 Jena, Germany
  • To the time point of the study, affiliated with Biomedical Optics Department, Laser Zentrum Hannover e.V., D-30419 Hannover, Germany and also with CrossBIT, Hannover Medical School, 30625 Hannover, Germany.
/ Bodo Rosenhahn
  • Institute for Information Processing, Leibniz University of Hannover, D-30167 Hannover, Germany
/ Alexander Krüger
  • Biomedical Optics Department, Laser Zentrum Hannover e.V., D-30419 Hannover, Germany
  • CrossBIT, Hannover Medical School, 30625 Hannover, Germany
Published Online: 2013-05-17 | DOI: https://doi.org/10.1515/bmt-2012-0044

Abstract

Novel surface coatings of percutaneous implants need to be tested in biocompatibility studies. The use of animal models for testing usually involves numerous lethal biopsies for the analysis of the implant-tissue interface. In this study, optical coherence tomography (OCT) was used to monitor the reaction of the skin to a percutaneous implant in an animal model of hairless but immunocompetent mice. In vivo optical biopsies with OCT were taken at days 7 and 21 after implantation and post mortem on the day of noticeable inflammation. A Fourier-domain OCT was programmed for spoke pattern scanning schemes centered at the implant midpoint to reduce motion artifacts during in vivo imaging. Image segmentation allowed the automatic detection and morphometric analysis of the skin contour and the subcutaneous implant anchor. On the basis of the segmentation, the overall refractive index of the tissue within one OCT data set was estimated as a free parameter of a fitting algorithm, which corrects for the curved distortion of the planar implant base in the OCT images. OCT in combination with the spoke scanning scheme and image processing provided time-resolved three-dimensional optical biopsies around the implants to assess tissue morphology.

Keywords: in vivo; motion artifacts; refractive index; scanning scheme; segmentation

Corresponding author: Sabine Donner, Biomedical Optics Department, Laser Zentrum Hannover e.V., D-30419 Hannover, Germany, Phone: +49 511 2788 325, Fax: +49 511 2788 100


Received: 2012-07-24

Accepted: 2013-04-12

Published Online: 2013-05-17

Published in Print: 2013-08-01


Citation Information: Biomedizinische Technik/Biomedical Engineering. Volume 58, Issue 4, Pages 359–367, ISSN (Online) 1862-278X, ISSN (Print) 0013-5585, DOI: https://doi.org/10.1515/bmt-2012-0044, May 2013

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