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Short Peptides in Minimalistic Biocatalyst Design

Krystyna L. Duncan
  • WestCHEM/Department of Pure and Applied Chemistry, University of Strathclyde, Thomas Graham Building, 295 Cathedral Street, Glasgow, G1 1XL, UK
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Rein V. Ulijn
  • WestCHEM/Department of Pure and Applied Chemistry, University of Strathclyde, Thomas Graham Building, 295 Cathedral Street, Glasgow, G1 1XL, UK
  • Advanced Science Research Center (ASRC) and Hunter College, City University of New York, 85 St Nicholas Terrace, New York, NY10031, USA
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2015-09-01 | DOI: https://doi.org/10.1515/boca-2015-0005


We review recent developments in the use of short peptides in the design of minimalistic biocatalysts focusing on ester hydrolysis. A number of designed peptide nanostructures are shown to have (modest) catalytic activity. Five features are discussed and illustrated by literature examples, including primary peptide sequence, nanosurfaces/scaffolds, binding pockets, multivalency and the presence of metal ions. Some of these are derived from natural enzymes, but others, such as multivalency of active sites on designed nanofibers, may give rise to new features not found in natural enzymes. Remarkably, it is shown that each of these design features give rise to similar rate enhancements in ester hydrolysis. Overall, there has been significant progress in the development of fundamental understanding of the factors that influence binding and activity in recent years, holding promise for increasingly rational design of peptide based biocatalysts.

Keywords : primary sequence; histidine; nano-surface/ scaffold; multivalency; metals; rational design; catalysis; ester hydrolysis


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About the article

Received: 2015-05-15

Accepted: 2015-08-05

Published Online: 2015-09-01

Citation Information: Biocatalysis, Volume 1, Issue 1, Pages 67–81, ISSN (Online) 2353-1746, DOI: https://doi.org/10.1515/boca-2015-0005.

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© 2015 Krystyna L. Duncan, Rein V.. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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