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Acta Botanica Croatica

The Journal of University of Zagreb

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Pseudo-nitzschia Peragallo (Bacillariophyceae) diversity and domoic acid accumulation in tuberculate cockles and sweet clams in M’diq Bay, Morocco

Benlahcen Rijat Leblad
  • Corresponding author
  • Laboratoire de Phytoplancton, Institut National de Recherche Halieutique, 90000 Tanger, Morocco
  • Faculté des Sciences et Techniques, Université Abdelmalek Essadi, Tanger, Morocco
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Nina Lundholm / Didier Goux / Benoît Veron / Regia Sagou / Hamid Taleb / Hassan Nhhala
  • Laboratoire de Phytoplancton, Institut National de Recherche Halieutique, 90000 Tanger, Morocco
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Hassan Er-Raioui
Published Online: 2013-04-13 | DOI: https://doi.org/10.2478/v10184-012-0004-x

Abstract

The diversity of Pseudo-nitzschia (Bacillariophyceae) and accumulation of the neurotoxin domoic acid (DA) in two types of shellfish; tuberculate cockles (Acanthocardia tuberculata) and sweet clams (Challista chione) was explored in M’diq Bay,Morocco during 2007. The highest abundances of Pseudo-nitzschia were found during the period from March to October, with peaks occurring in May and September. Toxin analysis showed an accumulation of domoic acid in shellfish sampled during spring and autumn. The maximum toxin concentration was 4.9 mg DAg-1 of the whole tissue recorded in sweet clam during spring. Using transmission electron microscopy, thirteen Pseudo-nitzschia species were identified, eight of which are known as producers of domoic acid: P. multistriata, P. cuspidata, P. galaxiae, P. multiseries, P. pseudodelicatissima, P. pungens var. aveirensis, P. calliantha and P. fraudulenta. The five non- toxic species observed were P. subpacifica, P. arenysensis, P. dolorosa, P. subfraudulenta, and P. cf. caciantha

Keywords: Diatoms; domoic acid; phytoplankton; Pseudo-nitzschia; shellfish; Morocco

  • AKALLAL, R.,CHANTAL, B., JACQUELINE, F.,THIERRY, C.,MOURADI,A., 2002: Contribution à l’étude du phytoplancton de la côte atlantique marocaine, II. Le Genre Pseudo-nitzschia (Bacillariophyceae). Cryptogamie, Algologie 23, 187-202.Google Scholar

  • ALVAREZ, G., URIBE, E., QUIJANO-SCHEGGIA, S., RIVERA, A. L., MARINO, C., BLANCO, J., 2009: Domoic acid production by Pseudo-nitzschia australis and Pseudo-nitzschiacalliantha isolated from North Chile. Harmful Algae 8, 938-945.Web of ScienceCrossrefGoogle Scholar

  • AMATO, A., LÜDEKING, A.,KOOISTRA,W. H., 2010: Intracellular domoic acid production in Pseudo-nitzschia multistriata isolated from the Gulf of Naples (Tyrrhenian Sea, Italy). Toxicon 55,157-161.PubMedCrossrefWeb of ScienceGoogle Scholar

  • AMZIL, Z., JAQUELINE, F.,DOMINIQUE, L. G.,CHANTAL, B., 2001: Domoic acid accumulation in French shellfish in relation to toxic species of Pseudo-nitzschia multiseries and P.pseudodelillcatissima. Toxicon 39, 1245-1251.CrossrefGoogle Scholar

  • BARGU, S., KORAY, T., LUNDDHOLM, N., 2002: First report of Pseudo-nitzschia calliantha Lundholm, Moestrup & Hassle 2003, a new potentially toxic species from Turkish coasts. Journal of Fisheries and Aquatic Sciences 19, 479-483.Google Scholar

  • BATES, S. S.,BIRD, C. J.,DE FREITAS, A. S.W., FOXALL, L. A.,HANIC, L. A. JOHNSON, G. R., MCCULLOCH, A. W.,ODENSE, P., POCKLINGTON, R.,QUILLIAM, M. A., SIM, P. G., SMITH, J. C., SUBBA, RAO, D. V.,TODD, E. C. D.,WALTER, J. A.,WRIGHT, J. L. C., 1989: Pennate diatom Nitzschia pungens as the primary source of domoic acid, a toxin in shellfish from eastern Prince Edward Island, Canada. Canadian Journal of Fisheries and Aquatic Sciences 46, 1203-1215.CrossrefGoogle Scholar

  • BESIKTEPE, S., LARISA, R., DILEK, E., DORUK, Y., ARIFE, Z., VITALY, R., RAISA, L., 2008: Domoic acid production by Pseudo-nitzschia calliantha Lundholm, Moestrup et Hasle (bacillariophyta) isolated from the Black Sea. Harmful Algae 7, 438-442.Web of ScienceCrossrefGoogle Scholar

  • BOURHILI, E., 1984: Intoxication alimentaire par les Moules. (51 cas). Agadir octobre et novembre 1982. Thèse no. 33, Faculté de Médecine et Pharmacie, Rabat.Google Scholar

  • CAROPPO, C.,CONGESTRI, R.,BRACCHINI, L.,ALBERTANO, P., 2005: On the presence of Pseudo--nitzschia calliantha Lundholm, moestrup et Hasle and Pseudo-nitzschia delicatissima (Cleve) Heiden in the Southern Adriatic Sea (Mediterranean Sea, Italy). Journal of Plankton Research 27, 163-774.Google Scholar

  • CERINO, F., ORSINI, L., SARNO, D., CARMELA, D. A., TARTAGLIONE, L., ZINGONE, A, 2005: The alternation of different morphotypes in the seasonal cycle of the toxic diatom Pseudo-nitzschia galaxiae. Harmful Algae 4, 33-48.CrossrefGoogle Scholar

  • ESSAID, E. F., 1977: L’intoxication paralytique par les fruits de mer. Thèse no. 44, Faculté Médecine et Pharmacie, Rabat.Google Scholar

  • HASLE, G. R., 2002: Are most of the domoic acid-producing species of the diatom genus Pseudo-nitzschia cosmopolites? Harmful Algae 1, 137-146.CrossrefGoogle Scholar

  • ILLOUL, H.,MASO, M., FORTUNO, J. M., CROS, L.,MORALES-BLAKE, A., SÉRIDJI, R., 2008: Potentially harmful microalgae in coastal waters of the Algiers area (Southern Mediterranean Sea). Cryptogamie, Algologie 29, 261-278.Google Scholar

  • INÈS, S.,ASMA, S. H.,BATES, S. S., 2006: First detection of toxic Pseudo-nitzschia Calliantha in Bizert lagoon, Tunisia. Harmful Algae News 30, 8-9.Google Scholar

  • KANIOU-GRIGORIADOU, I., MOURATIDOU, T., KATIKOU, P., 2005: Investigation on the presence of domoic acid in Greek shellfish. Harmful Algae 4, 717-723.CrossrefGoogle Scholar

  • KLEIN, C., CLAQUIN, P., BOUCHART, V., LE ROY, B.,VÉRON, B., 2010: Dynamics of Pseudo--nitzschia spp. and domoic acid production in a macrotidal ecosystem of the Eastern English Channel (Normandy, France). Harmful Algae 9, 218-226.CrossrefWeb of ScienceGoogle Scholar

  • LJUBEŠIĆ, Z.,BOSAK, S.,VILI^I], D.,KRALJ BOROJEVI], K.,MARI], D.,GODRIJAN, J.,UJEVI], I., PEHAREC, P.,DJAKOVAC, T., 2011: Ecology and taxonomy of potentially toxic Pseudo--nitzschia species in Lim Bay (north-eastern Adriatic Sea).Harmful Algae 10, 713-722.CrossrefGoogle Scholar

  • LOUREIRO, S.,GARÉS, E., FERNANDZ, T.M.,VAQUÉ, D.,CAMP, J., 2009: Pseudo-nitzschia spp. (Bacillariophyceae) and dissolved organic matter (DOM) dynamics in the Ebro Delta (Alfacs Bay, NW Mediterranean Sea). Estuarine, Coastal Shelf Science 83, 539-549.Web of ScienceGoogle Scholar

  • LUNDHOLM, N., (ed.) 2011: Bacillariophyta, in IOC-UNESCO Taxonomic Reference List of Harmful Micro Algae. Retrieved June 11, 2012, from http://www.marinespecies.org/ HAB LUNDHOLM, N.,NEILS, D.,OJVIND, M., 2002: Phylogeny of the Bacillariaceae with emphasis on the genus Pseudo-nitzschia (Bacillariophyceae) based on partial LSU rDNA. Journal of Phycology 37, 115-134.Google Scholar

  • MARGALEF, R., 1969: Composicion especifica del fitoplancton de la costa Catalano-levantina (mediterraneo occidental)) en 1962-1967. Investigacion of Pesquera 33, 345-380.Google Scholar

  • MARIĆ, D.,LJUBE[I], Z.,GODRIJAN, J.,VILI^I], D.,UJEVI], I.,PRECALI, R., 2011: Blooms of the potentially toxic diatom Pseudo-nitzschia calliantha Lundholm, Moestrup & Hasle in coastal waters of the northern Adriatic Sea (Croatia). Estuarine Coastal and Shelf Science 92, 323-331.CrossrefWeb of ScienceGoogle Scholar

  • MARTIN, J. L.,HAYA, K., BURRIDGE, L. E.,WILDISH, D. J., 1990: Nitzschia pseudodelicatissma a source of domoic acid in the Bay of Fundy, eastern Canada.Marine Ecology Progress Series 67, 177-182.CrossrefGoogle Scholar

  • MASSUTI, M.,MARGALEF, R., 1950: Introducciyn al studio del plankton marino. Patronato juan de la Cierva de Investigacion técnica, Barcelona.Google Scholar

  • MOSCHANDREOU, K. K., PAPAEFTHIMIOU, D.,KATIKOU, P.,KALOPESA, E., PANOU, A.,NIKOLAIDIS, G., 2010: Morphology, phylogeny and toxin analysis of Pseudo-nitschia pseudodelicatissima (Bacillariophyceae) isolated from the Thermaikos Gulf, Greece. Phycologia 49, 260-273.Web of ScienceCrossrefGoogle Scholar

  • ORSINI, L.,DIANA, S.,GABREILLE, P.,ROBERTO, P., JENS, D.,MARINA,M., 2002: Toxic Pseudo--nitzschia multistriata (Bacillariophyceae) from the Gulf of Naples: morphology, toxin analysis and phylogenetic relationships with other Pseudo-nitzschia species. European Journal of Phycology 37, 247-257.CrossrefGoogle Scholar

  • PAN, Y., SUBBA, R. D. V.,MANN, K. H.,LI,W. K.W.,HARRISON,W. G., 1996: Effects of silicate limitation on production of domoic acid, a neurotoxin, by the diatom Pseudo-nitzschia multiseries. II. Continuous culture studies. Marine Ecology Progress Series 131, 235-243.CrossrefGoogle Scholar

  • PAN, Y., MICHAEL, L. P., MARK, B., MOELLER, P. D. R., QUAY DORTCH, POWELL, C. L., DOUCETTE, G. J., 2001: Pseudo-nitzschia sp. cf. pseudodelicatissima a confirmed producer of domoic acid from the northern Gulf of Mexico. Marine Ecology Progress Series 22, 83-92.CrossrefGoogle Scholar

  • QUIJANO-SCHEGGIA, S., ESTHER, G., NAGORE, S., KEES, V. L., EVA, F., KARL, A., JOSÉ- -MANUEL, F., JORDI, C., 2008: Identification and characterisation of the dominant Pseudo-nitzschia species (Bacillariophyceae) along the NE Spanish coast (Catalonia, NW Mediterranean). Scientia Marina 72, 343-359.Google Scholar

  • QUIJANO-SCHEGGIA, S., ESTHER, G.,KARL, B. A., PABLO, D. L. I., JORGE, D., JOSE-MANUEL, F., JORDI, C., 2010: Pseudo-nitzschia species on the Catalan coast: characterization and contribution to the current knowledge of the distribution of this genus in the Mediterranean Sea. Scientia Marina 74, 395-410.Web of ScienceCrossrefGoogle Scholar

  • QUILLIAM, M. A.,XIE, M.,HARDSTAFF,W. R., 1995: Rapid extraction and cleanup for liquid chromatographic determination of domoic acid in unsalted food. Journal of Aoac International 78, 543-554.Google Scholar

  • RHODES, L. L., JANET, A., CHRIS, S., 2000: Pseudo-nitzschia multistriata (Bacillariophyceae) in New Zealand; New Zealand Journal of Marine and Freshwater Research 34, 463-467.Web of ScienceCrossrefGoogle Scholar

  • RHODES, L. L.,WHITE, D., SYHRE, M., ATKINSON, M., 1996: Pseudonitzschia species isolated from New Zealand coastal waters: domoic acid production in vitro and links with shellfish toxicity. In: YASUMOTO, T., OSHIMA, Y., FUKUYO, Y. (ed), Harmful and toxic algal blooms, 155-158. Intergovernmental Oceanographic Commission of UNESCO, Paris.Google Scholar

  • RHODES, L. L., 1998: Identification of potentially toxic Pseudo-nitzschia (Bacillariophyceae) in New Zealand coastal waters, using lectins; New Zealand Journal ofMarine and Freshwater Research 32, 537-544.Google Scholar

  • SAGOU, R.,AMNHIR, R.,TALEB, H.,VALE, P.,BLAGHEN, M.,LOUTFI,M., 2005: Comparative study on differential accumulation of PSP toxins between cockle (Aconthocardia tubercula) and sweet clam (Callista chione). Toxicon 46, 612-618.CrossrefGoogle Scholar

  • SARNO, D., DAHLMANN, J., 2000: Production of domoic acid in another species of Pseudo--nitzschia: P. multistriata in the Gulf of Naples (Mediterranean Sea). Harmful Algae News 21.Google Scholar

  • SPATHARIS, S.,DANIELIDIS, D. B.,TSIRTSIS, G., 2007: Recurrent Pseudo-nitzschia calliantha (Bacillariophyceae) and Alexandrium insuetum (Dinophyceae) winter blooms induced by agricultural runoff. Harmful Algae 6, 811-822.CrossrefGoogle Scholar

  • TALEB, H., VALE, P., JAIME, E., BLAGHEN, M., 2001: Study of paralytic shellfish poisoning toxin profile in shellfish from the Mediterranean shore of Morocco. Toxicon 39, 1855-1861.PubMedCrossrefGoogle Scholar

  • TRAINER, V. L.,ADAMS, N. G.,BILL, B. D., STEHR, C. M.,WEKELL, J. C.,MOELLER, P.,BUSMAN, M., WOODRUFF, D., 2000: Domoic acid production near California coastal upwelling zones. Limnology and Oceanography 45, 1818-1833.CrossrefGoogle Scholar

  • TRAINER, V. L.,HICKEY, B. M.,LESSARD, E. J.,COCHLAN,W. P.,TRICK, C. G.,WELLS, M. L., MACFADYEN, A.,MOORE S. K., 2009: Variability of Pseudo-nitzschia and domoic acid in the Juan de Furca eddy region and its adjacent shelves. Limnology and Oceanography 51, 289-308.CrossrefGoogle Scholar

  • UJEVIĆ, I., NINČEVIĆ-GLADAN, @., ROJE, R., SKEJI], S., ARAPOV, J., MARASOVI], I., 2010: Domoic acid - A new toxin in the Croatian Adriatic shellfish toxin profile. Molecules 15, 6835-6849.PubMedWeb of ScienceCrossrefGoogle Scholar

  • UTERMÖHL, H., 1958: Zur Vervollkommnung der quantitative Phytoplankton Methodik. Mitteilungen Internationale Vereinigung für Theoretische und Angewandte Limnologie 9, 1-38.Google Scholar

  • WRIGHT, J. L. C.,BOYD, R. K.,FREITAS, A. S.W.,FALK,M.,FOXALL, R. A., JAMIESON,W. D., LAYOCK, M. V., MCCULLOCH, A. W., MACLNNES, A. G., ODENSE, P., PATHAK, V. P., QUILLIAM, M. A., RAGAN, M. A., SIM, P. G., THIBAUT, P.,WALTER, J. A., GILGAN, M., RICHARD, D. J. A.,DEWAR, D., 1989: Identification of domoic acid, a neurotoxic amino acid, in mussels from eastern Prince Edward Island. Canadian Journal of Chemistry 67, 481-490.CrossrefGoogle Scholar

  • ZAPATA, M., RODRIGUEZ, F., FRAGA, S., BARRA, L., RUGGIERO, M. V., 2011: Chlorophyll C pigment patterns in 18 species (51 strains) of the genus Pseudo-nitzschia (Bacillariophyceae). Journal of Phycology 47, 1274-1280.CrossrefWeb of ScienceGoogle Scholar

  • ZINGONE, A., SIANO, R., D’ALELIO, D., SARNO, D., 2006: Potentially toxic and harmful microalgae from coastal waters of the Campania region (Tyrrhenian Sea, Mediterranean Sea). Harmful Algae 5, 321-337.CrossrefGoogle Scholar

About the article

Published Online: 2013-04-13

Published in Print: 2013-04-01


Citation Information: Acta Botanica Croatica, Volume 72, Issue 1, Pages 35–47, ISSN (Print) 0365-0588, DOI: https://doi.org/10.2478/v10184-012-0004-x.

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