Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Acta Botanica Croatica

The Journal of University of Zagreb

2 Issues per year

IMPACT FACTOR 2016: 0.516
5-year IMPACT FACTOR: 0.694

CiteScore 2016: 0.79

SCImago Journal Rank (SJR) 2016: 0.269
Source Normalized Impact per Paper (SNIP) 2016: 0.722

Open Access
See all formats and pricing
More options …

Chemical composition and antioxidant potential of Acacia leucophloea Roxb

Muhammad Zia-Ul-Haq
  • Research Institute of Pharmaceutical Sciences, Department of Pharmacognosy, University of Karachi, Karachi 75270, Pakistan
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Sanja Ćavar
  • University of Sarajevo, Faculty of Science, Department of Chemistry, 71000 Sarajevo, Bosnia and Herzegovina
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Mughal Qayum / Inamullah Khan / Shakeel Ahmad
Published Online: 2013-04-13 | DOI: https://doi.org/10.2478/v10184-012-0005-9


The aim of this study was to evaluate the compositional and nutritional potential of methanolic extracts of various parts of Acacia leucophloea Roxb. concerning the chemical composition and antioxidant potential of which limited information is available. Compositional studies indicated carbohydrates as major components in both seed and pods. Despite differences in mineral content among the leaves, pods and seeds, calcium was found in the highest amount and zinc in the lowest. The amino acid profile indicated aspartic acid as the major amino acid and proline as the minor. Among protein fractions, globulin was present in higher amounts than other fractions. Linoleic acid was the major fatty acid detected in the oil from both pods and seeds, while g-tocopherol was the major component of the tocopherol observed from same oil. Moreover, significant antioxidant potential was observed from the extracts of all three parts investigated. The results obtained in this study clearly indicate that A. leucophloea has a sufficient potential for use as a natural antioxidant agent. Further phytochemical studies will be performed for specification of the biologically active principles.

Keywords : Acacia leucophloea; mineral content; amino acids; fatty acids; tocopherols; sterols; antioxidant activity

  • AKHTAR, S., 1992: Chemical and nutritional evaluation of genus acacia of the family Leguminosae of pakistan. PhD Thesis, University of Punjab Lahore, Pakistan.Google Scholar

  • AOAC, 1990: Official methods of analysis of the Association of Official Analytical Chemists. Association of Official Analytical Chemists, Washington, DC.Google Scholar

  • BALZ, M., SHULTE, E., THEIR, H. P., 1992: Trennung von Tocopherolen und Tocotrienolen durch HPLC. Lipid/ Fett 94, 209-213.Google Scholar

  • BENZIE, I. F. F., STRAIN, J. J., 1999: Ferric reducing/antioxidant power assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods in Enzymology 299, 15-27.Google Scholar

  • DAVID, S., SEIGLER, J., EBINGER, E., 1987: Cyanogenic glycosides in ant-acacias of Mexico and central America. The Southwestern Naturalist 32, 499-503.Google Scholar

  • FALADE, O. S., ADEKUNLE, A. S., ADEROGBA, M. A., ATANDA, S. O., HARWOOD, C., ADEWUSI, S. R., 2008: Physicochemical properties, total phenol and tocopherol of some Acacia seed oils. Journal of Science of Food and Agriculture 88, 263-268.Google Scholar

  • FREIDMAN, M., FINELY, J.W., 1971:Methods of tryptophan analysis. Journal of Agriculture Food Chemistry 19, 626-631.Google Scholar

  • GHISELLI, A., SERAFINI, M., MAIANI, G., AZZINI, E., FERRO-LUZZI, A., 1995: A fluorescence- based method for measuring total plasma antioxidant capability. Free Radical Biology and Medicine 18, 29-36.Google Scholar

  • GRACE, S. C., 2005: Phenolics as antioxidants. In SMIRNOFF, N. (ed.), Antioxidants and reactive oxygen species in plants, 141-168. Blackwell Publishing, Oxford.Google Scholar

  • KHALIL, I. A., DURANI, F. R.,1990: Haulm and Hull of peas as a protein source in animal feed. Sarhad Journal of Agriculture 6, 219-225.Google Scholar

  • KHARE, C. P., 2007: Indian medicinal plants, an illustrated dictionary. Springer, Stuttgart.Google Scholar

  • KUMAR, J. I. N., KUMAR, R. N., BHOI, R. K., PATEL, K., 2010: Seasonal changes of bioelements in litter and their potential return to green leaves in five species of tropical dry deciduous forest, western India. Journal of Forestry Research 21, 33-38.Google Scholar

  • LOWRY, O. H.,ROSENBROUGH, N. J., FARR, A. L.,RANDALL, R. J., 1951: Protein measurement with Folin phenol reagent. Journal of Biological Chemistry 1993, 265-275.Google Scholar

  • NASIR, E.,ALI, S. I., 1974: Flora of west Pakistan. University of Karachi, Feroz Sons Press, Karachi.Google Scholar

  • MURRAY, D. R., 1979: The seed proteins of kowhai, Sophora microphylla AIT. Z. Pflazenphysiology 93, 423-428.Google Scholar

  • ORWA, C.,MUTUA, A., KINDT, R., JAMNADASS, R., SIMONS, A., 2009: Agroforestree. A tree reference and selection guide, version 4.0. Retrieved July 02, 2012, from http://www. worldagroforestry.org/treedb2/AFTPDFS/Acacia_leucophloea.pdfGoogle Scholar

  • PELLEGRINI, N., SERAFINI, M., COLOMBI, B., DEL RIO, D., SALVATORE, S., BIANCHI, M., BRIGHENTI, F. 2003: Total antioxidant capacity of plant foods, beverages and oils consumed in Italy assessed by three different in vitro assays. Journal of Nutrition 133, 2812-2819.Google Scholar

  • RAJARAM, N., JANARDHANAN, K., 1990: Chemical composition and nutritional evaluation of certain under-exploited Vigna spp. Food Science and Nutrition 42, 213-221.Google Scholar

  • RUBANZA, C. D. K., SHEM, M. N.,BAKENGESA, S. S., ICHINOHE, T., FUJIHARA, T., 2007: The content of protein, fiber and minerals of leaves of selected Acacia species indigenous to north-western Tanzania. Archive in Animal Nutrition 61, 151-156.Google Scholar

  • POLHILL, R. M., RAVEN, P. H., 1981: Advances in legume systematics, Royal Botanic Gardens, Kew and Ministry of Agriculture, Fisheries and Food, London.Google Scholar

  • SAINI, M. L., SAINI, R., ROY, S., KUMAR, A., 2008: Comparative pharmacognostical and antimicrobial studies of acacia species (Mimosaceae). Journal of Medicinal Plants Research 12, 378-386.Google Scholar

  • SELVANAYAGAM, Z. E.,GNAVAVENDHAN, S. G.,BALAKRISHNA, K.,BHIMA, R. R.,USMAN, S. A., 1995: Survey of medicinal plants with anti snake venom activity in Chengalpattu district, Tamilnadu, India. Fitoterapia 66, 488-494.Google Scholar

  • SHANG-TZEN, C., JYH-HORNG,W.,SHENG-YANG,W.,PEI-LING, K.,NING-SUN, Y.,LIE-FEN, S., 2001: Antioxidant activity of extracts from Acacia confusa bark and heartwood. Journal of Agriculture and Food Chemistry 49, 3420-3424.Google Scholar

  • SIDDHURAJU, P., VIJAYAKUMARI, K., JANARDBANAN, K., 1996: Chemical composition and nutritional evaluation of an underexploited legume, Acacia nilotica (L.).Del. Food Chemistry 57, 385-391.Google Scholar

  • TROUP, R. S., 1983: Troup’s silviculture of Indian trees, 4. Leguminosae. Forest Research Institute and Colleges, Dehra Dun, India.Google Scholar

  • VIJAYAKUMARI, K., SIDDHURAJU, P., JANARDHANAN, K., 1994: Nutritional assessment and chemical composition of the lesser known tree legume, Acacia leucophloea (Roxb.) Willd. Food Chemistry 50, 285-288.Google Scholar

  • WALTER, B. M., NASCIMENTO, M. C., PEREIRA, B. M., PEREIRA, N. A., 2000: Plant natural products active against snake bite,the molecular approach. Phytochemistry 55, 627-642.Google Scholar

  • ZIA-UL-HAQ, M.,AHMAD, S. SHAD, M. A., IQBAL, S.,QAYUM, M.,AHMAD, A., LUTHRIA, D. L,. AMAROWICZ, R., 2011: Compositional studies of some of lentil cultivars commonly consumed in Pakistan. Pakistan Journal of Botany 43, 1563-1567.Google Scholar

  • ZIA-UL-HAQ, M.,AHMAD, S.,AHMAD, M., IQBAL, S.,KHAWAR, K.M., 2009: Effects of cultivar and row spacing on tocopherol and sterol composition of chickpea (Cicer arietinum L.) seed oil. Tarim Bilimeri Dergisi 15, 25-30.Google Scholar

  • ZIA-UL-HAQ, M.,AHMAD, S.,CHIAVARO, E.,MEHJABEEN M., AHMED, S., 2010: Studies of oil from cowpea (Vigna unguiculata (L.) Walp.) cultivars commonly grown in Pakistan. Pakistan Journal of Botany 42, 214-220.Google Scholar

  • ZIA-UL-HAQ, M., IQBAL, S., AHMAD, M., 2008a: Characteristics of oil from seeds of 4 mungbean (Vigna radiate L.Wilczek) cultivars grown in Pakistan. Journal of American Oil Society 85, 851-856.Web of ScienceGoogle Scholar

  • ZIA-UL-HAQ, M., IQBAL, S.,AHMAD, S.,BHANGER, M. I.,WICZKOWSKI, W.,AMAROWICZ, R., 2008b: Antioxidant Potential of Desi Chickpea varieties commonly consumed in Pakistan. Journal of Food Lipids 15, 326-342.Web of ScienceGoogle Scholar

  • ZIA-UL-HAQ, M., IQBAL, S.,AHMAD, S., IMRAN, M.,NIAZ, A.,BHANGER,M. I., 2007: Nutritional and compositional study of Desi Chickpea (Cicer arietinum L.) cultivars grown in Punjab, Pakistan. Food Chemistry 105, 1357-1363.Web of ScienceGoogle Scholar

About the article

Published Online: 2013-04-13

Published in Print: 2013-04-01

Citation Information: Acta Botanica Croatica, Volume 72, Issue 1, Pages 133–144, ISSN (Print) 0365-0588, DOI: https://doi.org/10.2478/v10184-012-0005-9.

Export Citation

This content is open access.

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

Comments (0)

Please log in or register to comment.
Log in