Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Acta Botanica Croatica

The Journal of University of Zagreb

2 Issues per year

IMPACT FACTOR 2016: 0.516
5-year IMPACT FACTOR: 0.694

CiteScore 2016: 0.79

SCImago Journal Rank (SJR) 2016: 0.269
Source Normalized Impact per Paper (SNIP) 2016: 0.722

Open Access
See all formats and pricing
More options …

Oxidative stress and antioxidant indices of the marine red alga Porphyra vietnamensis

Navnath M. Pise
  • Corresponding author
  • Biological Oceanography Division, National Institute of Oceanography, Council of Scientific and Industrial Research, Dona Paula, Goa 403004, India
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Dattatry K. Gaikwad / Tanaji G. Jagtap
  • Biological Oceanography Division, National Institute of Oceanography, Council of Scientific and Industrial Research, Dona Paula, Goa 403004, India
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2013-10-08 | DOI: https://doi.org/10.2478/v10184-012-0024-6


-Oxidative stress and antioxidant defence systems were assessed in a marine red alga Porphyra vietnamensis Tanaka et Pham-Hoang Ho, from India. Lipid peroxidation (LPX) and hydrogen peroxide (H2O2) were measured as oxidative stress markers. Antioxidant defences were measured as catalase (CAT), glutathione S-transferase (GST) and ascorbic acid (AsA), in order to understand their dissimilarity with respect to environmental conditions (pollution levels) from selective locations along the central west coast of India. Levels of LPX, H2O2, CAT and GST were significantly higher in samples collected from Dona Paula than in samples from Malvan and Kunkeshwar, while a lower concentration of AsA was found in samples from Dona Paula. Heavy metals such as Cd, Pb and Hg in higher concentrations in these areas than in other sites were also observed. Variation of oxidative stress indices in response to the accumulation of heavy metals within P. vietnamensis could be used as molecular biomarkers for the assessment and monitoring of environmental quality in ecologically sensitive marine habitats.

Keywords : Porphyra vietnamensis; lipid peroxidation; antioxidant; biomarker; oxidative stress

  • AEBI, H., 1974: Catalase. In: BERGMEYER, H. U. (ed.), Methods in enzymatic analysis, 2, 673-678. Academic Press, New York.Google Scholar

  • ARAVIND, P., PRASAD, M. N. V., 2005: Modulation of cadmium-induced oxidative stress in Ceratophyllum demersum by zinc involves ascorbate-glutathione cycle and glutathione metabolism. Plant Physiology and Biochemistry 43, 107-116.CrossrefGoogle Scholar

  • ARUOMA, I. O., 1998: Free radicals, oxidative stress and antioxidants in human health and disease. Journal of American Oil Chemists Society 75, 199 - 221.Google Scholar

  • BAKER, C. J., ORLANDI, E. W., 1995: Active oxygen in plant pathogenesis. Annual Review of Phytopathology 33, 299-321.Google Scholar

  • BURTIN, P., 2003: Nutritional value of seaweeds. Electronic Journal of Environmental Agricultural and Food Chemistry 2, 498-503.Google Scholar

  • CONTI, M. E.,CECCHETTI, G. A., 2003: Biomonitoring study: trace metals in algae and molluscs from Tyrrhenian coastal areas. Environmental Research 93, 99-112.Google Scholar

  • COLLEN, J., DAVISON I. R., 1999a: Stress tolerance and reactive oxygen metabolism in the intertidal seaweeds Mastocarpus stellatus and Chondrus crispus. Plant Cell and Environment 22, 1143 - 1151.CrossrefGoogle Scholar

  • COLLEN, J., DAVISON, I. R., 1999b: reactive oxygen production and damage in intertidal Fucus spp. (Phaeophyceae). Journal of Phycology 35, 54-61.Google Scholar

  • COLLEN, J., PINTO, E., PEDERSEN, M.,COLEPICOLO, P., 2003: Induction of oxidative stress in the red macroalgae Gracillaria tenuistipitata by pollutant metals. Archives of Environmental Contamination and Toxicology 45, 337-342.Google Scholar

  • CPCB (Central Pollution Control Board), 1996: Coastal pollution. Technical publication Government of India, New Delhi, 30.Google Scholar

  • DAVISON, I. R., PEARSON, G. A. 1996: Environmental stress in intertidal seaweeds. Journal of Phycology 32, 197-211.Google Scholar

  • DAZY, M.,MASFARAUD, J. F., FERARD, J. F., 2009: Induction of oxidative stress biomarkers associated with heavy metal stress in Fontinalis antipyretica Hedw. Chemosphere 75, 297-302.Google Scholar

  • DIETZ, K. J., BAIER, M., KRAMER, U., 1999: Free radicals and reactive oxygen species as mediators of heavy metal toxicity in plants. In: PRASAD, M. N. V.,HAGEMEYER, J. (eds.), Heavy metal stress in plants - from molecules to ecosystems, 73-97. Springer-Verlag, Berlin.Google Scholar

  • DEHADRAI, P. V.,BHARGAVA, R.M. S. 1972: Seasonal organic production in relation to environmental features in Mandovi and Zuari estuaries, Goa. Indian Journal of Marine Sciences 1, 52-56.Google Scholar

  • FOYER, C. H., NOCTOR, G., 2000: Oxygen processing in photosynthesis: Regulation and signaling. New Phytologist 146, 359-388.Google Scholar

  • GOUIA, H., SUZUKI, A., BRULFERT, J., GHORBAL, M. H., 2003: Effects of cadmium on the co-ordination of nitrogen and carbon metabolism in bean seedlings. Journal of Plant Physiology 160, 367-376.Google Scholar

  • HABIG,W. H.,PABST,M. J., JAKOBY,W. B., 1974: Glutathione S-transferases: The first enzymatic step in mercapturic acid formation. Journal of Biological Chemistry 249, 7130-7139. Google Scholar

  • HALLIWELL, B., GUTTERIDGE, J. M. C., 1999: Oxydative stress. In: HALLIWELL, B., GUTTERIDGE, J. M. C. (eds.), Free radicals in biology and medicine, 2, 246-350. Oxford University Press, New York.Google Scholar

  • HALLIWELL, B.,GUTTERIDGE, J. M. C., 2001: Reactive species can be poisonous. In: HALLIWELL, B., GUTTERIDGE, J. M. C. (eds.), Free radicals in biology and medicine, 4, 440-487. Oxford University Press, New York.Google Scholar

  • HEATH, R. L., PACKER, L., 1968: Photoperoxidation in isolated chloroplasts I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics 125, 189-198.Google Scholar

  • KUMAR, S. V., SARITHA, G., FAREEDULLAH,M. D., 2010: Role of antioxidants and oxidative stress in cardiovascular disease. Annals of Biological Research 1, 158-173.Google Scholar

  • KUZNIAK, E.,MARIA, S., 2001: Ascorbate, glutathione and related enzymes in chloroplasts of tomato leaves infected by Botrytis cinerea. Plant Science 160, 723-731.Google Scholar

  • LEE, M. Y., SHIN, H.W., 2003: Cadmium-induced changes in antioxidant enzymes from the marine alga Nannochloropsis oculata. Journal of Applied Phycology 15, 13-19.CrossrefGoogle Scholar

  • LESSER, P. M., 2006: Oxidative stress in marine environments: biochemistry and physiological ecology. Annual Review Physiology 68, 253-278Google Scholar

  • LIU, Y.,WANG, X., ZENG, G.,QU, D.,GU, J., ZHOU, M., CHAI, L., 2007: Cadmium-induced oxidative stress and response of the ascorbate-glutathione cycle in Bechmeria nivea (L) Gaud. Chemosphere 69, 99-107.Google Scholar

  • LOBBAN, C. S., HARRISON, P. J., 1994: Seaweed ecology and physiology. Cambridge University Press, New York.Google Scholar

  • LOWRY, O. H.,ROSEBROUGH, N. J., FARR, A. L.,RANDALL, R. J., 1951: Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193, 265-275.Google Scholar

  • MAHARANA, D., JENA, K. B., PISE, N. M., JAGTAP, T. G., 2010: Assessment of oxidative stress indices in a marine macro brown alga Padina tetrastromatica (Hauck) from comparable polluted coastal regions of the Arabian Sea, west coast of India. Journal of Environmental Sciences 22, 1413-1418.CrossrefGoogle Scholar

  • MEDINA, M.,ANDRADE, S., FAUGERON, S.,LAGOS, N.,MELLA, D.,CORREA, J. A., 2005: Biodiversity of rocky intertidal benthic communities associated with copper mine tailing discharges in northern Chile. Marine Pollution Bulletin 50, 396-409.Google Scholar

  • MISHRA, S., TRIPATHI, R. D., SRIVASTAVA, S.,DWIVEDI, S., TRIVEDI, P. K.,DHANKHER, O. P., KHARE, A., 2009: Thiol metabolism play significant role during cadmium detoxification by Ceratophyllum demersum L. Bioresource Technology 100, 2155-2161.CrossrefPubMedGoogle Scholar

  • MITTLER, R. 2002: Oxidative stress, antioxidants and stress tolerance. Trends in Plant Science 7, 405-410.Google Scholar

  • MITUSI, A.,OHATA, T., 1961: Photooxidative consumption and photoreductive formation of ascorbic acid in green leaves. Plant Cell Physiology 2, 31-44.Google Scholar

  • MOACIR, A., TORRES, M., BARROS, P., SARA, C. G., CAMPOS-ERNANI, P., SATISH, R., RICHARD, T. S., PIO, C., 2008: Ã Biochemical biomarkers in algae and marine pollution: A review. Ecotoxicology and Environmental Safety 71, 1- 15.Google Scholar

  • MOON, J., SHIBAMOTO, T., 2009: Antioxidant assays for plant and food components. Journal of Agricultural Food Chemistry 57, 1655-1666. CrossrefGoogle Scholar

  • MORILLO, J., USERO, G., GRACIA, I., 2004: Heavy metal distribution in marine sediments from the southwest of Spain. Chemosphere 55, 431-442.Google Scholar

  • NIES, D. H., 1999: Microbial heavy metal resistance. AppliedMicrobiology and Biotechnology 51, 730-750.Google Scholar

  • NIMPTSCH, J.,WUNDERLIN, D. A., DOLLAN, A., PFLUGMACHER, S., 2005: Antioxidant and biotransformation enzymes in Myriophyllum quitense as biomarkers of heavy metal exposure and eutrophication in Suquia River basin (Codoba, Argentina), Chemosphere 261, 147-157.Google Scholar

  • ORBEA, A., ORTIZ-ZARRAGOITIA, M., SOLE, M., PORTE, C., CAJARAVILLE, M., 2002: Antioxidant enzymes and peroxisome proliferation in relation contaminant body burdens of PAHs and PCBs in bivalvia molluscs, crabs and fish from Urdiabai and Plentzia estuaries (Bay of Biscay). Aquatic Toxicology 58, 75-98.Google Scholar

  • PFLUGMACHER, S., 2004: Promotion of oxidative stress in the aquatic macrophyte Ceratophyllumdemersum during biotransformation of the cyanobacterial toxin microcystin- -LR. Aquatic Toxicology 70, 169-178.CrossrefGoogle Scholar

  • PFLUGMACHER, S.,WIENCKE, C., SANDERMANN, H., 1999: Activity of phase I and phase II detoxication enzymes in Antarctic and Arctic macroalgae. Marine Environmental Research 48, 23-36.CrossrefGoogle Scholar

  • PINTO, E., SIGAUD-KUTNER, T. C. S., LEITAO, M. A., OKAMOTO, O. K., MORSE, D., COLEPICOLO, P., 2003: Heavy metal-induced oxidative stress in algae. Journal of Phycology 39, 1008-1018.Google Scholar

  • QASIM, S. Z., SEN GUPTA, R., 1981: Environmental characteristics of the Mandovi - Zuari Estuarine system in Goa. Estuarine Coastal and Shelf Science 13, 557-578.Google Scholar

  • RAMA-DEVI, S., PRASAD, M. N. V., 1998: Copper toxicity in Ceratophyllum demersum L. (Coontail), a free floating macrophyte: response of antioxidant enzymes and antioxidants. Plant Science 138, 157-165.Google Scholar

  • REDDY, A. M.,KUMAR, S. G., JYOTHSNAKUMARI, G., THIMMANAIK, S., SUDHAKAR, C., 2005: Lead induced changes in antioxidant metabolism of horsegram (Macrotyloma uniflorum Lam. Verdc.) and bengalgram (Cicer arietinum L.). Chemosphere 60, 97-104.Google Scholar

  • ROY, S., LINDSTOM-SEPPA, P.,HUUSKONEN, S.,HANNINEN, O., 1995: Responses of biotransformation and antioxidant enzymes in Lemna minor and Oncorhynchus mykiss exposed simultaneously to hexachlorobenzene. Chemosphere 30, 1489-1498.Google Scholar

  • SALGADO, L. T., ANDRADE, L. R., AMADO-FILHO, G. M., 2005: Localization of specific monosaccharide in cells of the brown alga Padina gymnospora and the relation to heavy-metal accumulation. Protoplasma 225, 132-128.Google Scholar

  • SAWIDIS, T., BROWN, M. T., ZACHARIDIS, G., SRATIS, I., 2001: Trace metal concentrations in marine macro algae from different biotopes in the Aegean Sea. Environment International 27, 43-47.Google Scholar

  • SERGIEV, I.,ALEXIEVA, V.,KARANOV, E., 1997: Effect of spermine, atrazine and combination between them on some endogenous protective systems and stress markers in plants, Proceedings of the Bulgarian Academy of Sciences 51, 121-124.Google Scholar

  • SINGBAL, S. Y. S., 1976: Diurnal variation of some physico-chemical factors in theMandovi estuary of Goa; Mahasagar Bulletin of the National Institute of Oceanography 9, 27-34. Google Scholar

  • SMEETS, K.,CUYPERS, A.,LAMBRECHTS, A., SEMANE, B.,HOET, P.,LAERE, A. V.,VANGRONSVELD, J., 2005: Induction of oxidative stress and antioxidativemechanisms in Phaseolusvulgaris after Cd application. Plant Physiology and Biochemistry 43, 437-444.Google Scholar

  • THOM, R.,DIXON, D. P.,EDWARDS, R.,COLE, D, L.,LAPTHORN,A. J., 2001: The structure of a zeta class glutathione S-transferase from Arabidopsis thaliana: characterisation of a GST with novel active-site tyrosine catabolism. Journal of Molecular Biology 308, 949-962.Google Scholar

  • VAN ASSCHE, F., CLIJSTERS, H., 1990: Effects of metals on enzyme activity in plants. Plant, Cell and Environment 13, 195-206.Google Scholar

  • WILLS, E. D., 1969: Lipid peroxide formation in microsomes, General considerations. Biochemical Journal 113, 315-324.Google Scholar

  • WOOD, J. M., 1974: Biological cycles for toxic elements in the environment. Science 183, 1049-1052.Google Scholar

  • ZAR, J. H., 1996: Biostatistical analysis. Prentice Hall, Upper Saddle River. Google Scholar

About the article

Published Online: 2013-10-08

Published in Print: 2013-10-01

Citation Information: Acta Botanica Croatica, Volume 72, Issue 2, Pages 197–209, ISSN (Print) 0365-0588, DOI: https://doi.org/10.2478/v10184-012-0024-6.

Export Citation

This content is open access.

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

Comments (0)

Please log in or register to comment.
Log in