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Acta Botanica Croatica

The Journal of University of Zagreb

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Variability of pollen aperture heteromorphism in annual pansies (Viola Section Melanium)

Sara Magrini / Anna Scoppola
  • Department of Agriculture, Forests, Nature and Energy (DAFNE), Tuscia University, via San Camillo de Lellis, I:01100 Viterbo, Italy
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Published Online: 2015-04-07 | DOI: https://doi.org/10.1515/botcro-2015-0003


Pollen heteromorphism is frequent in the section Melanium of the genus Viola, in which over 80% of the species produces pollen morphs with 3 to 6 apertures. Some authors have pointed out that many factors can affect the proportion of the different pollen types in perennial species, and that this proportion can change among populations. This work focuses on the study of the polymorphic pollen assemblage of three annual pansies: Viola arvensis, V. kitaibeliana, and V. hymettia, and on the assessment of its variability both within a population and within the same plant. In all the species, with both large and small flowers, 3-, 4- and 5-aperturate pollen grains were observed, with a large prevalence of 4-aperturate types. No pollen grains with 6 apertures were found. No significant variability of the pollen assemblage among flowers of the same plant was observed. In addition, in these three Viola species the frequencies of the various pollen morphs are also fairly constant among plants of the same population.

Keywords : annual pansies; pollen morph; pollen heteromorphism; section Melanium; variability; Viola


  • CLAPHAM, A. R., TUTIN, T. G., MOORE, D. M., 1987: Flora of the British Isles. 3rd ed., 107-112. Cambridge University Press, Cambridge.Google Scholar

  • CLAUSEN, J., CHANNELL, R. B., NUR, U., 1964: Viola rafinesquii, the only Melanium violet native to North America. Rhodora 66, 32-46.Google Scholar

  • DAJOZ, I., MIGNOT, A., HOSS, C., TILL-BOTTRAUD, I., 1995: Pollen aperture heteromorphism is not due to unreduced gametophytes. American Journal of Botany 82, 104-111.Google Scholar

  • DAJOZ, I., TILL-BOTTRAUD, I., GOUYON, P. H., 1991: Evolution of pollen morphology. Science 253, 66-68.Google Scholar

  • DAJOZ, I., TILL-BOTTRAUD, I., GOUYON, P. H., 1993: Pollen aperture polymorphism and gametophyte performance in Viola diversifolia. Evolution 47, 1080-1093.CrossrefGoogle Scholar

  • DAVIS, P. H., MILL, R. R., TAN, K., 1988: Viola L. In: DAVIS, P. H., MILL, R. R., TAN, K. (eds.), Flora of Turkey and the East Aegean Islands (Suppl. 1), Vol. 10, 62-64. Edinburgh University Press, Edinburgh.Google Scholar

  • ERBEN, M., 1985: Cytotaxonomische Untersuchungen an sudosteuropeischen Viola-Arten der Sektion Melanium. Mitteilungen der Botanischen Staatssammlung Munchen 21, 339-740.Google Scholar

  • ERDTMAN, G., 1952: Pollen morphology and plant taxonomy. Almqvist & Wiksell, Stockholm.Google Scholar

  • ESPEUT, M., 1999: Errata et addenda de l’article: ≫Approche du genre Viola dans le Midi Mediterraneen francais≪. Le Monde des Plantes 467, 7-9.Google Scholar

  • ESPEUT, M., 2004: Viola roccabrunensis sp. nov. Le Monde des Plantes 482, 18-21.Google Scholar

  • GAMS, H., 1926: Viola L. In: HEGI, G., Illustrierte Flora von Mitteleuropa, vol. 5, 586-656. Lehmanns Verlag, Munchen.Google Scholar

  • KRISTOFFERSON, K. B., 1923: Crossings in Melanium-violets. Hereditas 4, 251-289.CrossrefGoogle Scholar

  • MAGRINI, S., SCOPPOLA, A., 2013: Is it possible to define the real Italian distribution area of the annual pansy, Viola kitaibeliana? In: PECCENINI, S., DOMINA, G. (eds.), Contributi alla ricerca floristica in Italia, 53-54. Societa Botanica Italiana, Palermo.Google Scholar

  • MARCUSSEN, T., KARLSSON, T., 2010: Violaceae. In: JONSELL, B., KARLSSON, T. (eds.), Flora Nordica, vol. 6, 12-52. Royal Swedish Academy of Sciences, Stockholm.Google Scholar

  • MERXMULLER, H., 1982: Viola L. In: PIGNATTI, S. (ed.), Flora d’Italia, Vol. 2, 102-118. Edagricole, Bologna.Google Scholar

  • MIGNOT, A., HOSS, C., DAJOZ, I., LEURET, C., HENRY, J. P., DREUILLAUX, J. M., HEBERLE-BORS, E., TILL-BOTTRAUD, I., 1994: Pollen aperture polymorphism in the angiosperms: importance, possible causes and consequences. Acta Botanica Gallica 141, 109-122.Google Scholar

  • NADOT, S., BALLARD, H. E. JR, CREACH, J. B., DAJOZ, I., 2000: The evolution of pollen heteromorphism in Viola: A phylogenetic approach. Plant Systematics and Evolution 223, 155-171.Google Scholar

  • PETTET, A., 1964: Studies on British pansies. II. The status of some intermediates between Viola tricolor L. and V. arvensis Murr. Watsonia 6, 51-69.Google Scholar

  • RANDALL, R. E., 2004: Biological flora of the British Isles no. 233: Viola kitaibeliana Schult(es). Journal of Ecology 92, 361-369.Google Scholar

  • RAUS, T. H., 1986: Viola L. In: STRID, A. (ed.), Mountain flora of Greece, Vol. I, 608-640. Cambridge University Press, Cambridge.Google Scholar

  • SAEIDI MEHRVARZ S., YOUSEFI N., MOHAMMADI M., MARCUSSEN T., 2014: Pollen studies in the genus Viola (Violaceae) from Iran. Acta Botanica Croatica 73, 93-106.Web of ScienceGoogle Scholar

  • SCOPPOLA, A., LATTANZI, E., 2012: Viola section Melanium (Violaceae) in Italy. New data on morphology of Viola tricolor-Group. Webbia 67, 47-64.CrossrefGoogle Scholar

  • TILL-BOTTRAUD, I., MIGNOT, A., DE PAEPE, R., DAJOZ, I., 1995: Pollen heteromorphism in Nicotiana tabacum (Solanaceae). American Journal of Botany 82, 1040-1048.Google Scholar

  • TILL-BOTTRAUD, I., VINCENT, M., DAJOZ, I., MIGNOT, A., 1999: Pollen aperture heteromorphism. Variation in pollen-type proportions along altitudinal transects in Viola calcarata. Comptes Rendus de l’Academie des Sciences - Series III - Sciences de la Vie 322, 579-589.Google Scholar

  • VALENTINE, D. H., MERXMULLER, H., SCHMIDT, A., 1968: Viola L. In: TUTIN, T. G., HEYWOOD, V. H., BURGES, N. A., MOORE, D. M., VALENTINE, D. H., WALTERS, S. M., WEBB, D. A. (eds.), Flora Europaea, vol. 2, 270-282. Cambridge University Press, Cambridge.Google Scholar

  • VOLLRATH, K. H., 2011: Viola in Nordostbayern. Retrieved May 3, 2013 from http://www. regnitzflora.de/Veilchen_20_12_10.pdf WERNER, K., 1988: Viola kitaibeliana Schultes auf der Schwellenburg bei Erfurt - ein Neufund fur die DDR. Hercynia 25, 142-143.Google Scholar

  • YOCKTENG, R., BALLARD, H. E. JR, MANSION, G., DAJOZ, I., NADOT, S., 2003: Relationships among pansies (Viola section Melanium) investigated using ITS and ISSR markers. Plant Systematics and Evolution 241, 153-170. Google Scholar

About the article

Published Online: 2015-04-07

Published in Print: 2015-03-01

Citation Information: Acta Botanica Croatica, Volume 74, Issue 1, Pages 43–52, ISSN (Online) 0365-0588, DOI: https://doi.org/10.1515/botcro-2015-0003.

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© by Sara Magrini. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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