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Botanica Marina

Editor-in-Chief: Dring, Matthew J.

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1437-4323
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Volume 53, Issue 5 (Oct 2010)

Issues

Significance of radical oxygen production in sorus development and zoospore germination in Saccharina japonica (Phaeophyceae)

Hiroyuki Mizuta
  • Laboratory of Aquaculture Genetics and Genomics, Faculty of Fisheries Sciences, Hokkaido University, Minato 3-1-1, Hakodate, Hokkaido 041-8611, Japan
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Hajime Yasui
  • Laboratory of Marine Industrial Science and Technology, Faculty of Fisheries Sciences, Hokkaido University, Minato 3-1-1, Hakodate, Hokkaido 041-8611, Japan
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2010-09-14 | DOI: https://doi.org/10.1515/bot.2010.047

Abstract

We investigated production of radical oxygen species (ROS) in sorus development of sporophyte discs and during zoospore germination of Saccharina japonica (Phaeophyceae) using the fluorescent dye 2′,7′-dichlorofluorescein diacetate (DCFH-DA). Quantitative analysis of ROS showed high intracellular production during the stages of zoosporangium formation when paraphyses elongated. In this stage, remarkable ROS release was observed. ROS production was also observed histologically (under a fluorescence microscope) in the elongating paraphyses and the sorus zoosporangia. The sorus had significantly higher phenol content, antioxidant capacity and ROS scavenging enzyme activities (ascorbate peroxidase, catalase, glutathione reductase and superoxide dismutase) than adjacent non-sorus blade sectors. Thus, intracellular ROS scavenging mechanisms are active during ROS production in sorus formation. In contrast, iodine content was lower in the sorus than in the adjacent non-sorus tissues, suggesting the existence of an antioxidant defense mechanism based on iodine efflux. We demonstrated that ROS production is an important phenomenon in the reproduction of the S. japonica sporophyte. Furthermore, ROS were also observed in the zoospore germination processes. We discuss the physiological and ecological roles of ROS production in sorus formation and zoospore germination.

Keywords: antioxidant; reactive oxygen; Saccharina; sorus; zoospore germination

About the article

Corresponding author


Received: 2010-04-06

Accepted: 2010-08-06

Published Online: 2010-09-14

Published Online: 2010-09-14

Published in Print: 2010-10-01


Citation Information: Botanica Marina, ISSN (Online) 1437-4323, ISSN (Print) 0006-8055, DOI: https://doi.org/10.1515/bot.2010.047.

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