Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Botanica Marina

Editor-in-Chief: Dring, Matthew J.


IMPACT FACTOR 2018: 0.919
5-year IMPACT FACTOR: 1.336

CiteScore 2018: 1.22

SCImago Journal Rank (SJR) 2018: 0.399
Source Normalized Impact per Paper (SNIP) 2018: 0.672

Online
ISSN
1437-4323
See all formats and pricing
More options …
Volume 55, Issue 6

Issues

Hormone-autonomous cell culture from cotydelonary tissue of the marine plant Cymodocea nodosa

Maite Zarranz Elso
  • Departamento de Biología, Facultad de Ciencias del Mar, Universidad de Las Palmas de Gran Canaria, 35017 Las Palmas de Gran Canaria, Canary Islands, Spain
  • Instituto Canario de Ciencias Marinas, Muelle de Taliarte, 35200, Telde, Gran Canaria, Canary Islands, Spain
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Nieves González-Henríquez
  • Instituto Canario de Ciencias Marinas, Muelle de Taliarte, 35200, Telde, Gran Canaria, Canary Islands, Spain
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Pilar García-Jiménez
  • Departamento de Biología, Facultad de Ciencias del Mar, Universidad de Las Palmas de Gran Canaria, 35017 Las Palmas de Gran Canaria, Canary Islands, Spain
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Rafael R. Robaina
  • Corresponding author
  • Departamento de Biología, Facultad de Ciencias del Mar, Universidad de Las Palmas de Gran Canaria, 35017 Las Palmas de Gran Canaria, Canary Islands, Spain
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2012-09-04 | DOI: https://doi.org/10.1515/bot-2012-0122

Abstract

In vitro germinated seedlings of ­Cymodocea nodosa were used as a source of young embryonic tissue for cell culture. Histological study of the cotyledon revealed the presence of meristematic tissue that gave rise to the first leaf of the plantlet, which released large numbers of cells after enzymatic digestion and thus indicated great growth potential. An effective enzymatic digestion protocol for C. nodosa meristem tissue was developed; this consisted of a 24-h digestion period at 28°C using a 0.6-m enzymatic solution (plasmolysis medium containing 1% (w:v) cellulase and hemicellulase). It was necessary to add citric acid (0.02 m) as an antioxidant to the enzymatic solution to obtain the highest cell yield and viability (mean±SE: 371.6±30.1×105 cells g-1 fw and 97.8±0.6%, respectively). NaCl and other salt components of the seawater were key factors in the establishment of axenic cell cultures and their further progress; their absence in the medium inhibited contaminant overgrowth. Nevertheless, cells maintained growth for 90 days, even without hormones and deprived of salts, although these salts might be necessary to allow the development of morphogenetic programmes desired for plant production. In this manner, we generated a basic tool for the future of C. nodosa in vitro culture that will allow the establishment and maintenance of hormone-autonomous cell cultures with strong potential in future biotechnology for marine higher plants.

Keywords: axenic; cells; cotyledon; culture; Cymodocea nodosa

About the article

Corresponding author: Rafael R. Robaina, Departamento de Biología, Facultad de Ciencias del Mar, Universidad de Las Palmas de Gran Canaria, 35017 Las Palmas de Gran Canaria, Canary Islands, Spain


Received: 2012-03-05

Accepted: 2012-08-01

Published Online: 2012-09-04

Published in Print: 2012-12-01


Citation Information: Botanica Marina, Volume 55, Issue 6, Pages 591–600, ISSN (Online) 1437-4323, ISSN (Print) 0006-8055, DOI: https://doi.org/10.1515/bot-2012-0122.

Export Citation

©2012 by Walter de Gruyter Berlin Boston.Get Permission

Comments (0)

Please log in or register to comment.
Log in