Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Botanica Marina

Editor-in-Chief: Dring, Matthew J.

IMPACT FACTOR 2018: 0.919
5-year IMPACT FACTOR: 1.336

CiteScore 2018: 1.22

SCImago Journal Rank (SJR) 2018: 0.399
Source Normalized Impact per Paper (SNIP) 2018: 0.672

See all formats and pricing
More options …
Volume 59, Issue 2-3


A taxonomic re-examination of Saccharina longipedalis (Laminariales, Phaeophyceae), an endemic kelp species around Lake Akkeshi in eastern Hokkaido, Japan

Norishige Yotsukura / Nobu Nagai / Tadashi Kawai
  • Wakkanai Fisheries Research Institute, Hokkaido Research Organization, Wakkanai 097-0001, Japan
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2016-05-25 | DOI: https://doi.org/10.1515/bot-2016-0006


The taxonomy of Saccharina longipedalis, a kelp species endemic to the brackish Lake Akkeshi in eastern Hokkaido, was reviewed. The external morphology of sporophytes collected from seven sites around the distribution boundary of S. longipedalis and Saccharina japonica var. diabolica were compared; it was confirmed that the diagnostic morphology of S. longipedalis, such as a long stipe and thin blade, was not solely characteristic of individuals that grew in and around the center of the lake, which is thought to be the unique distribution area of S. longipedalis. Sequence comparisons of a 5S rDNA spacer and four microsatellite regions and AFLP analysis revealed that genetic similarity was high between S. longipedalis and S. japonica var. diabolica. Also based on observations that the early development in hybrids of sporelings from the seven sites was normal, we conclude that S. longipedalis should be treated as S. japonica var. diabolica.

Keywords: Japanese kelp; Lake Akkeshi; Saccharina japonica var. diabolica; Saccharina longipedalis; taxonomic re-examination


  • Duggins, D.O., J.E. Eckman, C.E. Siddon and T. Klinger. 2003. Population, morphometric and biomechanical studies of three understory kelps along a hydrodynamic gradient. Mar. Ecol. Prog. Ser. 265: 57–76.Google Scholar

  • Fowler-Walker, M.J., S.D. Connell and B.M. Gillanders. 2005. To what extent do geographic and associated environmental variables correlate with kelp morphology across temperate Australia? Mar. Freshw. Res. 56: 877–887.Google Scholar

  • Funano, T. 1991. Morpholoical and ecological comparisons of Laminaria religiosa, a survival variation of L. japonica and that of L. ochotensis from Oshoro, Sutts and Atsuta. Suisanzoshoku 39: 91–95 (in Japanese).Google Scholar

  • Kawashima, S. 1994. Taxonomic and distributional remarks on the laminariaceous algae (Laminariales, Phyaeophyta) of Japan-47. Aquabiology 91: 103–106 (in Japanese).Google Scholar

  • Kawashima, S. 1995a. Taxonomic and distributional remarks on the laminariaceous algae (Laminariales, Phyaeophyta) of Japan-51. Aquabiology 95: 464–467 (in Japanese).Google Scholar

  • Kawashima, S. 1995b. Taxonomic and distributional remarks on the laminariaceous algae (Laminariales, Phyaeophyta) of Japan-52. Aquabiology 95: 7–10 (in Japanese).Google Scholar

  • Kawashima, S. 1995c. Taxonomic and distributional remarks on the laminariaceous algae (Laminariales, Phyaeophyta) of Japan-53. Aquabiology 97: 111–115 (in Japanese).Google Scholar

  • Lane, C.E., C. Mayes, L.D. Druehl and G.W. Saunders. 2006. A multi-gene molecular investigation of the kelp (Laminariales, Phaeophyceae) supports substantial taxonomic re-organization. J. Phycol. 42: 493–512.Google Scholar

  • Maeda, T. and N. Yotsukura. 2013. Development of microsatellite markers for Saccharina japonica by dual-suppression PCR. Algal Res. 6: 67–71.Google Scholar

  • Maeda, T., T. Kawai, M. Nakaoka and N. Yotsukura. 2013. Effective DNA extraction method for fragment analysis using capillary sequencer of the kelp, Saccharina. J. Appl. Phycol. 25: 337–347.Web of ScienceGoogle Scholar

  • Miyabe, K. 1902. Laminariaceae. Hokkaido Suisan Chosahokoku Vol. 3. Hokkaido Shokuminbu, Sapporo. pp. 216 (in Japanese).Google Scholar

  • Nabata, S., E. Abe and M. Kakiuchi. 1993. Ecological study and transplanting experiment of the biennial plants of Laminaria on the coast of Isoya, Suttu, southernwestern Hokkaido, Japan. Sci. Rep. Hokkaido Fish. Exp. Stn. 43: 25–35.Google Scholar

  • Notoya, M. and S. Kirihara. 1989. Morphology of wild and transplantation plants of Laminaria japonica at the coast of Aomori prefecture. Kaiyoukagaku 21: 355–361 (in Japanese).Google Scholar

  • Okamura, K. 1891. On seaweeds of Hokkaido. Dainippon Suisankaihokoku 114: 584–590 (in Japanese).Google Scholar

  • Okamura, K. 1896. On Laminaria of Japan. Bot. Mag. Tokyo 10: 87–101.Google Scholar

  • Okamura, K. 1916. Nippon Sorui Meii. Seibido, Tokyo. pp. 276 (in Japanese).Google Scholar

  • Sanbonsuga, Y. 1978. On the morphological characteristics of Laminaria japonica var. japonica studied by transplanting experiment. II. On the varieties of Laminaria japonica var. japonica Aresch. Bull. Hokkaido Reg. Fish. Res. Lab. 43: 79–88.Google Scholar

  • Sanbonsuga, Y. and S. Torii. 1973. On the morphological characteristics of Laminaria japonica var. japonica studied by transplanting experiment. I. On local forms of Laminaria japonica var. japonica. Bull. Hokkaido Reg. Fish. Res. Lab. 39: 61–82.Google Scholar

  • Sanbonsuga, Y. and S. Torii. 1974. On the local variation of morphological characteristics in Laminaria japonica Areschoug var. japonica. Bull. Hokkaido Reg. Fish. Res. Lab. 40: 48–59.Google Scholar

  • Ueda, S. 1933. Marine botany. Kouseikaku, Tokyo. pp. 319 (in Japanese).Google Scholar

  • Yendo, K. 1911. Studies of marine vegetation. Hakubunkan, Tokyo. pp. 748 (in Japanese).Google Scholar

  • Yoon, H.S., J.Y. Lee, S.M. Boo and D. Bhattacharya. 2001. Phylogeny of Alariaceae, Laminariaceae, and Lessoniaceae (Phaeophyceae) based on plastid-encoded RuBisCo spacer and nuclear-encoded ITS sequence comparisons. Mol. Phylogenet. Evol. 21: 231–243.Google Scholar

  • Yoshida, T., M. Suzuki and K. Yoshinaga. 2015. Check list of marine algae of Japan (Revised in 2015). Jpn. J. Phycol. 63: 129–189 (in Japanese).Google Scholar

  • Yotsukura, N. 2005. Molecular phylogeny of advanced kelps (Laminariales, Phaeophyceae) growing in Japan. Nat. Hist. Res. 8: 69–81.Google Scholar

  • Yotsukura, N. and L.D. Druehl. 2009. A new name for Laminaria sachalinensis (Laminariales, Phaeophyceae). J. Jpn. Bot. 84: 113–117.Google Scholar

  • Yotsukura, N., T. Denboh, T. Motomura, T. Horiguchi, A.W. Coleman and T. Ichimura. 1999. Little divergence in ribosomal DNA internal transcribed spacer –1 and –2 sequences among non-digitate species of Laminaria (Phaeophyceae) from Hokkaido, Japan. Phycol. Res. 47: 71–80.Google Scholar

  • Yotsukura, N., T. Kawai, S. Kawashima, H. Ebata and T. Ichimura. 2006. Nucleotide sequences diversity of the 5S rDNA spacer in the simple blade genera Laminaria, Cymathaere and Kjellmaniella (Laminariales, Phaeophyceae) from northern Japan. Phycol. Res. 54: 269–279.Google Scholar

  • Yotsukura, N., S. Kawashima, T. Kawai, T. Abe and L.D. Druehl. 2008. A systematic re-examination of four Laminaria species: L. japonica, L. religiosa, L. ochotensis and L. diabolica. J. Jpn. Bot. 83: 165–176.Google Scholar

  • Yotsukura, N., T. Shimizu, T. Katayama, L.D. Druehl. 2010. Mitochondrial DNA sequence variation of four Saccharina species (Laminariales, Phaeophyceae) growing in Japan. J. Appl. Phycol. 22: 243–251.Google Scholar

  • Yotsukura, N., T. Maeda, T. Abe, M. Nakaoka and T. Kawai. 2016. Detection of genetic difference among varieties in Saccharina japonica in northern Japan by DNA fragment analyses. J. Appl. Phycol.CrossrefGoogle Scholar

About the article

Norishige Yotsukura

Norishige Yotsukura is an Associate Professor in Field Science Center for Northern Biosphere, Hokkaido University. He also serves as the Director of Oshoro Marine Station, Hokkaido University. His research interests are on biodiversity, conservation of natural resources and breeding of kelp.

Nobu Nagai

Nobu Nagai earned both his Bachelor’s degree and Master’s degree from Hokkaido University. His Master’s research was focused on biodiversity of kelp in the Russian Far East. He currently works in a leading baking company in Japan.

Tadashi Kawai

Tadashi Kawai is an Assistant Chief of the aquaculture and resources enhancement section on seaweeds, shellfish and sea urchin in Hokkaido Research Organization. He also has interests in biology, taxonomy, and conservation of freshwater crayfish.

Received: 2016-02-09

Accepted: 2016-04-28

Published Online: 2016-05-25

Published in Print: 2016-06-01

Citation Information: Botanica Marina, Volume 59, Issue 2-3, Pages 205–210, ISSN (Online) 1437-4323, ISSN (Print) 0006-8055, DOI: https://doi.org/10.1515/bot-2016-0006.

Export Citation

©2016 by De Gruyter.Get Permission

Comments (0)

Please log in or register to comment.
Log in