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Licensed Unlicensed Requires Authentication Published by De Gruyter July 19, 2017

Evaluating the effect of medium composition and fermentation conditions on microbial oil production by a Fusarium strain isolated from the open ocean

  • Tian-Hua Zhong

    Tian-Hua Zhong is an assistant professor at Third Institute of Oceanography, State Oceanic Administration, China. His current research focuses on marine oleaginous microorganisms.

    , Jia-Wei Zhang

    Jia-Wei Zhang received his Master’s degree from Fujian Medical University, China. His research is on microbial oil production from marine fungi.

    , Wei Xu

    Wei Xu is currently an assistant professor inKey Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, China. Dr Xu’s research focuses on fungal diversity and nitrogen related microorganisms in deep-sea environments by deep sequencing and metagenomic analysis.

    , Liying Huang

    Liying Huang is currently a professor in School of Pharmacy, Fujian Medical University, China. Her research interest focuses on bioactive compounds from medicinal plants.

    and Zhu-Hua Luo

    Zhu-Hua Luo is currently a professor at Third Institute of Oceanography, State Oceanic Administration, China. His research interest includes fungal diversity in marine environments and biodegradation of organic pollutants by marine fungi.

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From the journal Botanica Marina

Abstract

As an important feedstock for biodiesel production, microbial oil has attracted considerable interest in recent years. Here, we investigated the effect of medium composition and culture conditions on cell growth and lipid accumulation of an oleaginous fungus Fusarium sp. 3A00501 on synthetic nitrogen-limited media. The optimal nitrogen source, carbon source, and C/N molar ratio in the medium for lipid accumulation were peptone, glucose, and 76, respectively. Additionally, the optimal initial pH of the medium and culture temperature for lipid production of the fungus were 6.0 and 30°C, respectively. Under the optimized fermentation conditions, the cell biomass decreased, but the lipid content and yield increased (by about 45% and 27%, respectively) compared with the unoptimized conditions. The microbial oil produced by the fungus mainly contained palmitic, elaidic, oleic, linolenic, heneicosanoic, and eicosadienoic acids. The unsaturated fatty acids accounted for about 65% of the total fatty acids. This high percentage of unsaturated fatty acids in the oil was similar to the plant oils commonly used in biodiesel production. These results suggest that Fusarium sp. 3A00501 might be a promising strain to provide lipids for biodiesel production.

About the authors

Tian-Hua Zhong

Tian-Hua Zhong is an assistant professor at Third Institute of Oceanography, State Oceanic Administration, China. His current research focuses on marine oleaginous microorganisms.

Jia-Wei Zhang

Jia-Wei Zhang received his Master’s degree from Fujian Medical University, China. His research is on microbial oil production from marine fungi.

Wei Xu

Wei Xu is currently an assistant professor inKey Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, China. Dr Xu’s research focuses on fungal diversity and nitrogen related microorganisms in deep-sea environments by deep sequencing and metagenomic analysis.

Liying Huang

Liying Huang is currently a professor in School of Pharmacy, Fujian Medical University, China. Her research interest focuses on bioactive compounds from medicinal plants.

Zhu-Hua Luo

Zhu-Hua Luo is currently a professor at Third Institute of Oceanography, State Oceanic Administration, China. His research interest includes fungal diversity in marine environments and biodegradation of organic pollutants by marine fungi.

Acknowledgements

This research work was financially supported by the National Key Basic Research Program of China (973 Program, 2015CB755903), the International Science and Technology Cooperation Program of China (2015DFA20500), and the National Natural Science Foundation of China (41376171).

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Received: 2016-9-26
Accepted: 2017-6-16
Published Online: 2017-7-19
Published in Print: 2017-7-26

©2017 Walter de Gruyter GmbH, Berlin/Boston

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