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Botanica Marina

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Volume 59, Issue 4 (Aug 2016)


First evidence of the deep-sea fungus Oceanitis scuticella Kohlmeyer (Halosphaeriaceae, Ascomycota) from the Northern Hemisphere

Joëlle Dupont
  • Institut de Systématique, Evolution et Biodiversité, ISYEB – UMR 7205 – CNRS, MNHN, UPMC, EPHE, Muséum national d’histoire naturelle, Sorbonne Universités, 57 rue Cuvier, CP39, 75005 Paris, France
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Enrico SchwabeORCID iD: http://orcid.org/0000-0003-1937-7564
Published Online: 2016-07-22 | DOI: https://doi.org/10.1515/bot-2016-0030


We report on a collection of the deep-sea fungus Oceanitis scuticella from recently sunken wood at abyssal depth in the Northwest Pacific Ocean. The fungus was originally described as wood-associated from the Angola Basin. Subsequently, it was also found on sunken wood in the Southwest Pacific Ocean. Ascomata and ascospores of O. scuticella were examined by scanning electron microscopy and light microscopy. The phylogenetic affiliation of the fungus within the Halosphaeriaceae was demonstrated using partial nuclear internal transcribed spacer (ITS) regions and large subunit (LSU) rDNA sequencing. Slight morphological differences between this collection and previously described material were observed concerning ascomata shape, namely the drop-shaped cavity, the form of the hypostroma and the thickness of the peridium. In addition, the ascospores were smaller than those reported in previous collections. LSU rDNA phylogeny provided a good support for the placement of the NW Pacific Ocean sample within O. scuticella. The ITS rDNA sequence of the present collection differed from those of the earlier collections by 2.5–3%, a value accepted for intraspecific variation in fungi. Based on the present material, some factors interpreted as indicators for true deep-sea fungi, such as the absence of bark or co-existing xylophagic bivalves in sunken wood, are discussed and their importance is considered as limited.

Keywords: deep sea; distribution; genetics; Halosphaeriaceae; morphology


  • Amon, D.J., A.G. Glover, H. Wiklund, L. Marsh, K. Linse, A.D. Rogers and J.T. Copley. 2013. The discovery of a natural whale fall in the Antarctic deep sea. Deep Sea Res. II 92: 87–96.Web of ScienceGoogle Scholar

  • Bienhold, C., P. Pop Ristova, F. Wenzhofer, T. Dittmar and A. Boetius. 2013. How deep-sea wood falls sustain chemosynthetic life. PLoS One 8: e53590.Google Scholar

  • Brandt, A. and M. Malyutina. 2012. The German-Russian deep-sea expedition KuramBio (Kurile Kamchatka Biodiversity Study): to the Kurile Kamchatka Trench and abyssal plain on board of the R/V Sonne, 223rd Expedition, July 21th-September 7th 2012. Biocenter Grindel and Zoological Museum, University of Hamburg, Hamburg (Online-Ressource (100 pp., 5.46 MB). Förderkennzeichen BMBF 03G0223A.Google Scholar

  • Bruun, A.F. 1958. General introduction to the reports and list of deep-sea stations. Galathea Rep. 1: 7–48.Google Scholar

  • Cunha, M.R., F.L. Matos, L. Genio, A. Hilario, C.J. Moura, A. Ravara and C.F. Rodrigues. 2013. Are organic falls bridging reduced environments in the deep sea? Results from colonization experiments in the gulf of Cadiz. PLoS One 8: e76688.Web of ScienceGoogle Scholar

  • Das, S., P.S. Lyla and S.A. Khan. 2006. Marine microbial diversity and ecology: importance and future perspectives. Curr. Sci. 90: 1325–1335.Google Scholar

  • Distel, D.L., A.R. Baco, E. Chuang, W. Morrill, C. Cavanaugh and C.R. Smith. 2000. Do mussels take wooden steps to deep-sea vents? Nature 403: 725–726.Google Scholar

  • Dupont, J., S. Magnin, F. Rousseau, M. Zbinden, G. Frebourg, S. Samadi, B.R. de Forges and E.B. Jones. 2009. Molecular and ultrastructural characterization of two ascomycetes found on sunken wood off Vanuatu islands in the deep Pacific ocean. Mycol. Res. 113: 1351–1364.Web of ScienceGoogle Scholar

  • Fagervold, S.K., P.E. Galand, M. Zbinden, F. Gaill, P. Lebaron and C. Palacios. 2012. Sunken woods on the ocean floor provide diverse specialized habitats for microorganisms. FEMS Microbiol. Ecol. 82: 616–628.Web of ScienceGoogle Scholar

  • Feldman, R.A., T.M. Shank, M.B. Black, A.R. Baco, C.R. Smith and R.C. Vrijenhoek. 1998. Vestimentiferan on a whale fall. Biol. Bull. 194: 116–119.Google Scholar

  • Inderbitzin, P., S. Landvik, M.A. Abdel-Wahab and M.L. Berbee. 2001. Aliquandostipitaceae, a new family for two new tropical ascomycetes with unusually wide hyphae and dimorphic ascomata. Am. J. Bot. 88: 52–61.Google Scholar

  • Jones, E.B.G. 2000. Marine fungi: some factors influencing biodiversity. Fungal Divers. 4: 53–73.Google Scholar

  • Jones, E.B.G. and R. Campion-Alsumard. 1970. Marine fungi on polyurethane covered plates submerged in the sea. Nova Hedwigia 19: 567–582.Google Scholar

  • Jones, E.B.G., J. Sakayaroj, S. Suetrong, S. Somrithipol and K.L. Pang. 2009. Classification of marine Ascomycota, anamorphic taxa and Basidiomycota. Fungal Divers. 35: 1–187.Google Scholar

  • Khan, S.S. and P. Manimohan. 2011. Diversity and abundance of marine fungi on driftwood collected from Kerala State and Lakshadweep Islands, India. Mycosphere 2: 223–229.Google Scholar

  • Kohlmeyer, J. 1968. The first Ascomycete from the deep sea. J. Elisha Mitchell Sci. Soc. 84: 239–241.Google Scholar

  • Kohlmeyer, J. 1977. New genera and species of higher fungi from the deep sea (1615–5315 m). Rev. Mycol. 41: 189–206.Google Scholar

  • Kohlmeyer, J. and E. Kohlmeyer. 1979. Marine mycology. The higher fungi. Academic Press, New York, NY. pp. 690.Google Scholar

  • Kohlmeyer, J., B. Bebout and B. Volkmann-Kohlmeyer. 1995. Decomposition of Mangrove wood by marine fungi and teredinids in Belize. Mar. Ecol. 16: 27–39.Google Scholar

  • Lorion, J., B. Buge, C. Cruaud and S. Samadi. 2010. New insights into diversity and evolution of deep-sea Mytilidae (Mollusca: Bivalvia). Mol. Phylogenet. Evol. 57: 71–83.Web of ScienceGoogle Scholar

  • Palacios, C., M. Zbinden, M. Pailleret, F. Gaill and P. Lebaron. 2009. Highly similar prokaryotic communities of sunken wood at shallow and deep-sea sites across the oceans. Microb. Ecol. 58: 737–752.Web of ScienceGoogle Scholar

  • Pante, E., L. Corbari, J. Thubaut, T.-Y. Chan, R. Mana, M.-C. Boisselier, P. Bouchet and S. Samadi. 2012. Exploration of the deep-sea fauna of Papua New Guinea. Oceanography 25: 214–225.Google Scholar

  • Raghukumar, S. 2004. The role of fungi in marine detrital processes. In: (N. Ramaiah, ed) Marine Microbiology: Facets and Opportunities. National Institute of Oceanography, Goa, India. pp. 91–101.Google Scholar

  • Raghukumar, C., S.R. Damare and P. Singh. 2010. A review on deep-sea fungi: occurrence, diversity and adaptations. Bot. Mar. 53: 479–492.Web of ScienceGoogle Scholar

  • Ramirez-Llodra, E., A. Brandt, R. Danovaro, B. De Mol, E. Escobar, C.R. German, L.A. Levin, P. Martinez Arbizu, L. Menot, P. Buhl-Mortensen, B.E. Narayanaswamy, C.R. Smith, D.P. Tittensor, P.A. Tyler, A. Vanreusel and M. Vecchione. 2010. Deep, diverse and definitely different: unique attributes of the world’s largest ecosystem. Biogeosciences 7: 2851–2899.Web of ScienceGoogle Scholar

  • Romano, C., J.R. Voight, J.B. Company, M. Plyuscheva and D. Martin. 2013. Submarine canyons as the preferred habitat for wood-boring species of Xylophaga (Mollusca, Bivalvia). Prog. Oceanogr. 118: 175–187.Web of ScienceGoogle Scholar

  • Sakayaroj J., K.-L. Pang and E.B.G. Jones. 2011. Multi-gene phylogeny of the Halosphaeriaceae: its ordinal status, relationships between genera and morphological character evolution. Fungal Divers. 46: 87–109.Web of ScienceGoogle Scholar

  • Samadi, S., L. Corbari, J. Lorion, S. Hourdez, T. Haga, J. Dupont, M.C. Boisselier and B. Richer de Forges. 2010. Biodiversity of deep-sea organisms associated with sunken-wood or other organic remains sampled in the tropical Indo-Pacific. Cah. Biol. Mar. 51: 459–466.Google Scholar

  • Schwabe, E., I. Bartsch, M. Błażewicz-Paszkowycz, N. Brenke, A.V. Chernyshev, N.O. Elsner, V. Fischer, A. Jażdżewska, M.V. Malyutina, D. Miljutin, M. Miljtuina, G.M. Kamenev, I. Karanovic, A. Maiorova and L. Würzberg. 2015. Wood-associated fauna collected during the KuramBio – expedition in the North West Pacific. Deep Sea Res. II 111: 376–388.Web of ScienceGoogle Scholar

  • Shearer, C.A., E. Descals, B. Kohlmeyer, J. Kohlmeyer, L. Marvanová, D. Padgett, D. Porter, H.A. Raja, J.P. Schmit, H.A. Thorton and H. Voglymayr. 2006. Fungal biodiversity in aquatic habitats. Biodivers. Conserv. 16: 49–67.Web of ScienceGoogle Scholar

  • Slepecky, R.A. and W.T. Starmer. 2009. Phenotypic plasticity in fungi: a review with observations on Aureobasidium pullulans. Mycologia 101: 823–832.Web of ScienceGoogle Scholar

  • Smith, C.R., H. Kukert, R.A. Wheatcroft, P.A. Jumars and J.W. Deming. 1989. Vent fauna on whale remains. Nature 341: 27–28.Google Scholar

  • Tamura, K., G. Stecher, D. Peterson, A. Filipski and S. Kumar. 2013. MEGA6: Molecular evolutionary genetics analysis version 6.0. Mol. Biol. Evol. 30: 2725–2729.Google Scholar

  • Tedersoo, L., T. Jairus, B.M. Hortons, K. Abarenkov, T. Suvi, I. Saari and U. Koljalg. 2008. Strong host preference of ectomycorrhizal fungi in a Tasmanian wet sclerophyll forest as revealed by DNA barcoding and taxon-specific primers. New Phytolog. 180: 479–490.Web of ScienceGoogle Scholar

  • Tsui, C.K.M. and K.D. Hyde. 2004. Biodiversity of fungi on submerged wood in a stream and its estuary in the Tai Ho Bay, Hong Kong. Fungal Divers. 15: 205–220.Google Scholar

  • Turner, R.D. and A.C. Johnson. 1971. Biology of marine wood-boring molluscs. In: (E.B.G. Jones and S.K. Eltringham, eds) Marine borers, fungi and fouling organisms of wood. Proceedings of the OECD workshop organised by the Committee investigating the preservation of wood in the marine environment, 27th March–3rd April, 1968. Organisation for Economic Co-operation and Development, Paris. pp. 259–301.Google Scholar

  • Vilgalys, R. and M. Hester. 1990. Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. J. Bacteriol. 172: 4238–4246.Google Scholar

  • Vilgalys, R. and B.L. Sun. 1994. Assessment of species distributions in Pleurotus based on trapping of airborne basidiospores. Mycologia 86: 270–274.Google Scholar

  • White, T.J., T. Bruns, S. Lee and J. Taylor. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: (M.A. Innis, D.H. Gelfand, J.J. Sninsky and T.J. White, eds.) PCR protocols: a guide to methods and applications. Academic Press, San Diego. pp. 315–322.Google Scholar

  • Wolff, T. 1979. Macrofaunal utilization of plant remains in the deep sea. Sarsia 64: 117–136.Google Scholar

  • Zbinden, M., M. Pailleret, J. Ravaux, S.M. Gaudron, C. Hoyoux, J. Lambourdiere, A. Waren, J. Lorion, S. Halary and S. Duperron. 2010. Bacterial communities associated with the wood-feeding gastropod Pectinodonta sp. (Patellogastropoda, Mollusca). FEMS Microbiol. Ecol. 74: 450–463.Google Scholar

About the article

Joëlle Dupont

Joëlle Dupont’s main research fields are fungal diversity and taxonomy. Her research uses molecular tools and multiple gene phylogenies for systematic and to describe the diversity of fungi from various habitats, particularly food such as cheeses, or fungi associated to plants such as endophytes from conifers or wheat and some plant pathogens, more rarely fungi from extreme environments such as rocks and the deep sea. Recently Joëlle and her group developed research about sexual reproduction of Penicillium and about their domestication to produce cheese. As an expert in microscopic fungi, Joëlle Dupont is involved in applied mycology in collaboration with the food industry.

Enrico Schwabe

For about 20 years Enrico Schwabe has specialized on the molluscan class Polyplacophora with a focus on the taxonomy and biogeography of this group. His more recent research includes biological interactions of marine animals, especially parasitism of Copepods with other invertebrates. Being involved in several deep-sea programs he also concentrates on the interaction among benthic organisms via isolated basins and the question of how far environmental parameters may influence biogeography, biodiversity and abundance of organisms.

Received: 2016-04-06

Accepted: 2016-06-28

Published Online: 2016-07-22

Published in Print: 2016-08-01

Citation Information: Botanica Marina, ISSN (Online) 1437-4323, ISSN (Print) 0006-8055, DOI: https://doi.org/10.1515/bot-2016-0030.

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