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Bulletin of the Veterinary Institute in Pulawy

The Journal of National Veterinary Research Institute in Pulawy

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Polymorphisms of Growth Hormone Gene in a Native Chicken Population: Association with Egg Production

Samaneh Gorji Makhsous
  • Corresponding author
  • Department of Animal Science, University of Guilan, 41635-1314 Rasht, Iran
  • Institute of Biotechnology, Shiraz University, 71441-65186 Shiraz, Iran
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Seyed Ziaeddin Mirhoseini / Mohammad Javad Zamiri
  • Department of Animal Science, College of Agriculture, Shiraz University, 71441-65186 Shiraz, Iran
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Ali Niazi
Published Online: 2013-03-15 | DOI: https://doi.org/10.2478/bvip-2013-0014


A total of 142 chicken blood samples were collected and a specific primer set was used to amplify a fragment of growth hormone locus using PCR. PCR products were digested with SacI and MspI restriction endonucleases. The amplified fragment digested with SacI enzyme revealed two “+” (wild type) and “-” (normal type) alleles with the frequency of 0.898 and 0.102, respectively. The amplified fragment digested with MspI enzyme revealed three A, B and C alleles with the frequency of 0.599, 0.102, and 0.299, respectively. Frequencies of +/+, +/- and -/- were 0.817, 0.162, and 0.021, respectively, and those of AA, AB, AC, BB, BC, and CC were 0.338, 0.113, 0.409, 0.007, 0.070, and 0.063, respectively, in the studied population. The results of 2 and likelihood ratio tests showed that this population was at Hardy-Weinberg equilibrium with respect to the marker locus. Marker-trait association analysis revealed statistically significant differences between “SacI-RFLP” genotypes for egg production and rate of laying eggs. The relationship between the molecular marker and these traits can be useful to improve the chicken breeding programmes.

Keywords: chicken; growth hormone; genes; polymorphism

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About the article

Published Online: 2013-03-15

Published in Print: 2013-03-01

Citation Information: Bulletin of the Veterinary Institute in Pulawy, Volume 57, Issue 1, Pages 73–77, ISSN (Online) 2300-3235, DOI: https://doi.org/10.2478/bvip-2013-0014.

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