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Bulletin of the Veterinary Institute in Pulawy

The Journal of National Veterinary Research Institute in Pulawy

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IMPACT FACTOR 2016: 0.462

SCImago Journal Rank (SJR) 2016: 0.235
Source Normalized Impact per Paper (SNIP) 2016: 0.364

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Online
ISSN
2300-3235
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Polymorphisms of Growth Hormone Gene in a Native Chicken Population: Association with Egg Production

Samaneh Gorji Makhsous
  • Corresponding author
  • Department of Animal Science, University of Guilan, 41635-1314 Rasht, Iran
  • Institute of Biotechnology, Shiraz University, 71441-65186 Shiraz, Iran
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Seyed Ziaeddin Mirhoseini / Mohammad Javad Zamiri
  • Department of Animal Science, College of Agriculture, Shiraz University, 71441-65186 Shiraz, Iran
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Ali Niazi
Published Online: 2013-03-15 | DOI: https://doi.org/10.2478/bvip-2013-0014

Abstract

A total of 142 chicken blood samples were collected and a specific primer set was used to amplify a fragment of growth hormone locus using PCR. PCR products were digested with SacI and MspI restriction endonucleases. The amplified fragment digested with SacI enzyme revealed two “+” (wild type) and “-” (normal type) alleles with the frequency of 0.898 and 0.102, respectively. The amplified fragment digested with MspI enzyme revealed three A, B and C alleles with the frequency of 0.599, 0.102, and 0.299, respectively. Frequencies of +/+, +/- and -/- were 0.817, 0.162, and 0.021, respectively, and those of AA, AB, AC, BB, BC, and CC were 0.338, 0.113, 0.409, 0.007, 0.070, and 0.063, respectively, in the studied population. The results of 2 and likelihood ratio tests showed that this population was at Hardy-Weinberg equilibrium with respect to the marker locus. Marker-trait association analysis revealed statistically significant differences between “SacI-RFLP” genotypes for egg production and rate of laying eggs. The relationship between the molecular marker and these traits can be useful to improve the chicken breeding programmes.

Keywords: chicken; growth hormone; genes; polymorphism

  • 1. Astrid A., Yoshie O., Yoshihito S., Keiich S., Koichi C., Yoshiaki O., Kazuo K.: Effects of GH gene polymorphism and sex on carcass traits and fatty acid compositions in Japanese Black cattle. Anim Sci J 2009, 80, 62-69.Google Scholar

  • 2. Barta A., Richards R.I., Baxter JD., Shine J.: Primary structure and evolution of rat growth hormone gene. Proc Natl Acad Sci USA 1981, 78, 4867-4871.Google Scholar

  • 3. Bernardini S., Argenton F., Vianello S., Colombo L., Bortolussi M.: Regulatory regions in the promoter and third intron of the growth hormone gene in rain- bow trout, “Oncorhynchus mykiss Walbaum”. Gen Comp Endocrinol 1999, 116, 261-271.Google Scholar

  • 4. Byrne C.R., Wilson B.W., Ward K.A.: The isolation and characterization of the ovine growth hormone gene. Aust J Biol Sci 1987, 40, 459-68.Google Scholar

  • 5. Das P., Meyer L., Seyfert H.M., Brockmann G., Schwerin M.: Structure of the growth hormone-encoding gene and its promoter in mice. Gene 1996, 169, 209-213.Google Scholar

  • 6. DeNoto F.M., Moore D.D., Goodman H.M.: Human growth hormone DNA sequence and mRNA structure: Possible alternative splicing. Nucleic Acids Res 1981, 9, 3719-3730.Google Scholar

  • 7. Feng X.P., Kuhnlein U., Aggrey S.E., Gavora J.S., Zadworny D.: Trait association of genetic markers in the growth hormone and growth hormone receptor gene in a White Leghorn strain. Poult Sci 1997, 76, 1770-1775.Google Scholar

  • 8. Fiddes J.C., Seeburg P.H., DeNoto F.M., Hallewell R.A., Baxter J.D., Goodman H.M.: Structure of genes for human growth hormone and chorionic somatomammotropin. Proc Natl Acad Sci USA 1979, 76, 4294-4298.Google Scholar

  • 9. Kansaku N., Nakada A., Okabayashi H., Guemene D., Kuhnlein U.: DNA polymorphism in the chicken growth hormone gene: Association with egg production. Anim Sci J 2003, 74, 243-244.Google Scholar

  • 10. Kansaku N., Hiyama G., Sasanami T., Zadworny D.: Prolactin and growth hormone in birds: Protein structure, gene structure and genetic variation. J Poult Sci 2008, 45, 1-6.Web of ScienceGoogle Scholar

  • 11. Kioka N., Manabe E., Abe M., Hashi H., Yato M., Okuno M., Yamano Y., Sakai H., Komano T., Utsumi K., Iritani A.: Cloning and sequencing of goat growth hormone gene. Agric Biol Chem 1989, 53, 1583-1587.Google Scholar

  • 12. Kuhnlein U., Liu N., Weigend S., Gavora J.S., Fairfull W., Zadworny D.: DNA polymorphisms in the chicken growth hormone gene: response to selection for disease resistance and association with egg production. Anim Genet 1997, 28, 116-123.Google Scholar

  • 13. Lagziel A., Lipkin E., Ezra E., Soller M., Weller J.I.: An MspI polymorphism at the bovine growth hormone (bGH) gene is linked to a locus affecting milk protein percentage. Anim Genet 1999, 30, 296-299.Google Scholar

  • 14. Lei M., Luo C., Peng X., Fang M., Nie Q., Zhang D., Yang G., Zhang X.: Polymorphism of growth-correlated genes associated with fatness and muscle fiber traits in chickens. Poult Sci 2007, 86, 835-842.Web of ScienceGoogle Scholar

  • 15. Liu H.C., Kung H.J., Fulton J.E., Morgan R.W., Cheng H.H.: Growth hormone interacts with the Marek’s disease virus SORF2 protein and is associated with disease resistance in chicken. Proc Natl Acad Sci USA 2001, 98, 9203-9208.Google Scholar

  • 16. Moore D.D., Marks A.R., Buckley D.I., Kapler G., Payvar F., Goodman H.M.: The first intron of the human growth hormone gene contains a binding site for glucocorticoid receptor. Proc Natl Acad Sci USA 1985, 82, 699-702.Google Scholar

  • 17. Mou L., Liu N., Zadworny D., Chalifour L., Kuhnlein U.: Presence of an additional PstI fragment in intron 1 of the intron 1 of the chicken growth hormone- encoding gene. Gene 1995, 160, 313-314.Google Scholar

  • 18. Nie Q., Stephen C.Y., Zhang X., Leung F.C., Yang G.: New variations in intron 4 of growth hormone gene in Chinese native chickens. J Hered 2002, 93, 277-279.Google Scholar

  • 19. Nie Q., Sun B., Zhang D., Luo C., Ishag N.A., Lei M., Yang G., Zhang X.: High diversity of the chicken growth hormone gene and effects on growth and carcass traits. J Hered 2005, 96, 698-703.Google Scholar

  • 20. Raymond M., Rousset F.: GENEPOP Version 1.2: Population genetics software for exact tests and ecumenicism. J Hered 1995, 86, 248-249.Google Scholar

  • 21. SAS: SAS user’s guide. Statistical version, 2004, SAS Institute Inc., Cary, North Carolina, USA.Google Scholar

  • 22. Slater E.P., Rabenau O., Karin M., Baxter J.D., Beato M.: Glucocorticoid receptor binding and activation of a heterologous promoter by dexamethasone by the first intron of the human growth hormone gene. Mol Cell Biol 1985, 5, 2984-2992.Google Scholar

  • 23. Stephen C.Y., Zhang X., Leung F.C.: Genomic growth hormone gene polymorphisms in native Chinese chickens. Exp Biol Med 2001, 226, 458-462.Google Scholar

  • 24. Tanaka M., Hosokawa Y., Watahiki M., Nakashima K.: Structure of the chicken growth hormone-encoding gene and its promoter region. Gene 1992, 112, 235-239.Google Scholar

  • 25. Vasilatos-Younken R., Zhou Y., Wang X., McMurtry J.P., Rosebrough W., Decuypere E., Buys N., Darras M., Geyten V., Tomas F.: Altered chicken thyroid hormone metabolism with chronic GH enhancement in vivo: consequences for skeletal muscle growth. J Endocrinol 2000, 166, 609-620.Google Scholar

  • 26. Vize P.D., Wells J.R.E.: Isolation and characterization of the porcine growth hormone gene. Gene 1987, 55, 339-344.Google Scholar

  • 27. Woychik R.P., Camper S.A., Lyons R.H., Horowitz S., Goodwin E.C., Rottoman F.M.: Cloning and nucleotide sequencing of the bovine growth hormone gene. Nucleic Acids Res 1982, 10, 7192-7210.Google Scholar

  • 28. Yan B., Deng X., Fei Q., Hu X., Wu C., Li N.: Association between single nucleotide polymorphisms of the chicken growth hormone gene and chicken growth and carcass traits. Chin Sci Bull 2003, 48, 1304-1307.Google Scholar

  • 29. Zhang X.L., Jiang X., Liu Y.P., Du H.R., Zhu Q.: Identification of AvaI polymorphisms in the third intron of GH gene and their associations with abdominal fat in chickens. Poult Sci 2007, 86, 1079-1083.Web of ScienceGoogle Scholar

About the article

Published Online: 2013-03-15

Published in Print: 2013-03-01


Citation Information: Bulletin of the Veterinary Institute in Pulawy, ISSN (Online) 2300-3235, DOI: https://doi.org/10.2478/bvip-2013-0014.

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