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Bulletin of the Veterinary Institute in Pulawy

The Journal of National Veterinary Research Institute in Pulawy

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Isolation and identification of Mycoplasma mycoides cluster strains from goats in Chongqing, China

Haoju Wang / Li Ni / Hongjun Yang
  • Department of Agriculture Resources Utilization, College of Resources and Environments, Southwest University, 400715 Chongqing, People's Republic of China
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Limin Xu / Ning Ma / Honglei Ding
Published Online: 2014-03-25 | DOI: https://doi.org/10.2478/bvip-2014-0002


In order to evaluate the prevalence of the Mycoplasma mycoides cluster in goats in Chongqing, China, an epidemiological survey in this area was carried out. A total of 68 samples were subjected to bacteria isolation on Hartley’s medium. Four isolates (three from lung tissue and one from nasal discharges) were recovered from the samples and identified as the Mycoplasma species by their morphological and biochemical characteristics. They were further confirmed by PCR using 16S rRNA specific primer pairs and by restriction enzyme analysis. In vitro antimicrobial susceptibility of the isolates indicated that some strains had developed resistance to the antibiotics tested. This is the first report on the isolation, identification, and molecular characterisation of Mycoplasma species isolated from goats in Chongqing. This study also revealed a prevalence of Mycoplasma species infection in goats in this area.

Keywords: goat; Mycoplasma mycoides cluster; isolation; biochemical characterisation; antimicrobial resistance.


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About the article

Published Online: 2014-03-25

Published in Print: 2014-03-01

Citation Information: Bulletin of the Veterinary Institute in Pulawy, Volume 58, Issue 1, Pages 11–15, ISSN (Online) 2300-3235, DOI: https://doi.org/10.2478/bvip-2014-0002.

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