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Bulletin of the Veterinary Institute in Pulawy

The Journal of National Veterinary Research Institute in Pulawy

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Chlorinated hydrocarbons residues in milk fat of selected farm animals from the north-eastern part of Poland

Renata Pietrzak-Fiećko
  • Corresponding author
  • Department of Commodities and Food Analysis, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland
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  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Michalina Gałgowska
  • Department of Commodities and Food Analysis, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Sylwia Bakuła
  • Department of Commodities and Food Analysis, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Barbara Felkner-Poźniakowska
  • Department of Commodities and Food Analysis, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2014-03-25 | DOI: https://doi.org/10.2478/bvip-2014-0011


The aim of the study was to determine and compare the concentrations of chlorinated hydrocarbons residues (DDT, DDE, DDD, γ-HCH) in the milk fat of selected species of farm animals. The experiment was carried out on cow’s, sheep’s, goat’s, and mare’s milk samples originating from different parts of north-eastern Poland. The samples were prepared using Röse-Gottlieb’s and Ludwicki’s methods. The determination of the compounds was conducted with gas chromatography. All tested samples contained the residues of chlorinated hydrocarbons. The results varied depending on the animal species as well as the places of sample collections. The highest content of γ-HCH and ΣDDT was determined in cow’s milk (22.75; 53.12 μg/kg of fat, respectively). The lowest level of γ-HCH and ΣDDT was observed in sheep’s milk (0.25; 5.94 μg/kg of fat, respectively). The content of chlorinated hydrocarbons did not exceed the maximum acceptable levels of these compounds.

Keywords: cow; sheep; goat; mare; milk; organochlorine compounds


  • 1. Banaszkiewicz T., Szarek J., Wysocki K.: Biological evaluation of soil contamination around a non-operating pesticide tomb. Pol J Environ Stud 2011, 20, 485-488.Google Scholar

  • 2. Barber J.L., Sweetman A., Van Wijk D., Jones K.C.: Hexachlorobenzene in the global environment: emission, levels, distribution, trends, and processes. Sci Total Environ 2005, 349, 1-44.Google Scholar

  • 3. Beard J.: Australian rural health research collaboration: DDT and human health. Sci Total Environ 2006, 355, 78-89.Google Scholar

  • 4. Borchers A., Teuber S.S., Keen C.L., Gershwin M.E.: Food safety. Clinical Clin Rev Allergy Immunol 2010, 39, 95-141.Google Scholar

  • 5. Businco L., Giampietro P.G., Lucenti P., Lucaroni F., Pini C., Di Felice G., Iacovacci P., Curadi C., Orlandi M.: Allergenicity of mare’s milk in children with cow’s milk allergy. J Allergy Clin Immunol 2000, 105, 1031-1034.Google Scholar

  • 6. Cerkvenik V., Doganoc D.Z., Jan J.: Evidence of some trace elements, organochlorine pesticides and PCBs in Slovenian cow’s milk. Food Technol Biotechnol 2000, 38, 155-160.Google Scholar

  • 7. Cohn B.A.: Developmental and environmental origins of breast cancer: DDT as a case study. Reprod Toxicol 2011, 31, 302-311.Web of ScienceGoogle Scholar

  • 8. Commission Regulation (EC) No 149/2008 of 29 January 2008 amending Regulation (EC) No 396/2005 of the European Parliament and of the Council by establishing Annexes II, III and IV setting maximum residue levels for products covered by Annex I thereto. http://ec.europa.eu/food/plant/protection/pesticides/legislation_en.htm.Google Scholar

  • 9. Dalvie M.A., Myers J.E., Thompson M.L., Dyer S., Robins T.G., Omar S., Riebow J., Molekwa J., Kruger P., Millar R.: The hormonal effects of long-term DDT exposure on malaria vector- control workers in Limpopo Province, South Africa. Environ Res 2004, 96, 9-19.Google Scholar

  • 10. Faruga A., Pietrzak-Fiećko R., Smoczyński S.S.: Chlorinated hydrocarbons in feed and tissues of turkey hens from a breeding flock and their content in egg yolks and blood of poults. Pol J Nat Sci 2010, 25, 259-266.Google Scholar

  • 11. Flores G.P., Gonzalez G.D., Tolentino R.G., Leon S.V., Perez M.N., Garcia E.C.: Organochlorine pesticide residues in goat milk from Queretaro, Queretaro, Mexico. Vet México 2007, 38, 291-301.Google Scholar

  • 12. John P.J., Bakore N., Bhatnagar P.: Assessment of organochlorine pesticide residue levels in dairy milk and buffalo milk from Jaipur City, Rajasthan, India, Environ Intern 2001, 26, 231-236.CrossrefGoogle Scholar

  • 13. Kaushik C.P., Sharma H.R., Gulati D., Kaushik A.: Changing patterns of organochlorine pesticide residues in raw bovine milk from Haryana, India. Environ Monit Assess 2011, 182, 467-475.Web of ScienceGoogle Scholar

  • 14. Ludwicki J.K., Góralczyk K., Czaja K., Strusiński P.: Determination of the residues of organochlorine insecticides and polychlorinated biphenyls in food products by gas chromatography method. PZH, Warszawa 1996.Google Scholar

  • 15. Niewiadowska A., Semeniuk S., Żmudzki J.: Residues of pesticides in animal origin food in 1997-2006 in Poland. Med Weter 2008, 64, 1221-1224.Google Scholar

  • 16. Pardio V.T., Waliszewski K.N., Landin L.A., Bautista R.G. Organochlorine pesticide residues in cow’s milk from a tropical region of Mexico. Food Add Contam 2003, 20, 259-269.CrossrefGoogle Scholar

  • 17. Pietrzak-Fiećko R., Smoczyński S., Tomczyński R.: Residues of chlorinated carbohydrates in animal and human milk. Med Weter 2000, 56, 715-717.Google Scholar

  • 18. Pietrzak-Fiećko R., Borejszo Z., Załuska O., Smoczyńska K., Tomczyński R., Smoczyński S.S.: Influence of location and lactation period on organochlorine insecticide content in mares’ milk from Poland. Bull Vet Inst Pulawy 2009, 53, 83-87.Google Scholar

  • 19. PN-EN ISO 1211:2002 Milk - determination of fat content. Gravimetric method (Method of appeal).Google Scholar

  • 20. PN-EN ISO 707:2009 Milk and dairy products. Guidelines for sampling.Google Scholar

  • 21. Polder A., Savinova T.N., Tkachev A., Løken K.B., Odland J.O., Skaare J.U.: Levels and patterns of persistent organic pollutants (POPS) in selected food items from Northwest Russia (1998-2002) and implications for dietary exposure. Sci Total Environ 2010, 408, 5352-5361.Google Scholar

  • 22. Purnomo A.S., Mori T., Kamei I., Kondo R.: Basic studies and applications on bioremediation of DDT: a review. Int Biodeterior Biodegrad 2011, 65, 921-930.Web of ScienceGoogle Scholar

  • 23. Rogan W.J., Chen A.: Health risk and benefits of bis (4-chlorophenyl)-1,1,1-trichloroethane (DDT). Lancet 2005, 366, 763-771.Google Scholar

  • 24. Snedeker S.M.: Pesticides and breast cancer risk: a review of DDT, DDE and dieldrin. Environ Health Persp 2001, 109, 35-47.Google Scholar

  • 25. Struciński P., Góralczyk K., Ludwicki J., Czaja K., Hernik A., Korcz W.: Levels of selected organochlorine insecticides, polychlorinated biphenyls, phthalates and perfluorinated aliphatic substances in blood - Polish WWF study. Roczniki PZH 2006, 57, 99-112.Google Scholar

  • 26. Tebourbi O., Hallcgue D., Yacoubi M.T., Sakly M., Rhouma K.B.: Subacute toxicity of p,p-DDT on rat thyroid: Hormonal and histopathological changes. Environ Toxicol Pharmacol 2010, 29, 271-279.Web of ScienceGoogle Scholar

  • 27. Van Dyk J.C., Bouwman H., Barnhoorn I.E.J., Bormann M.S.: DDT contamination from indoor residual spraying for malaria control. Sci Total Environ 2010, 408, 2745-2752.Web of ScienceGoogle Scholar

  • 28. Vidaeff A.C., Sever L.E.: In utero exposure to environmental estrogens and male reproductive health: a systematic review of biological and epidemiologic evidence. Reprod Toxicol 2005, 20, 5-20. CrossrefGoogle Scholar

About the article

Published Online: 2014-03-25

Published in Print: 2014-03-01

Citation Information: Bulletin of the Veterinary Institute in Pulawy, Volume 58, Issue 1, Pages 71–75, ISSN (Online) 2300-3235, DOI: https://doi.org/10.2478/bvip-2014-0011.

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© 2013. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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