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Bulletin of the Veterinary Institute in Pulawy

The Journal of National Veterinary Research Institute in Pulawy

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IMPACT FACTOR 2016: 0.462

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Adriamycin activity's durational governance of different cell death types and zonality in rat liver acinus. Immunohistochemical studies

Agnieszka Pedrycz
  • Corresponding author
  • Department of Histology and Embryology with Laboratory of Experimental Cytology, Medical University of Lublin, 20-080 Lublin, Poland
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/ Zbigniew Boratyński
  • Department of Animal Anatomy and Histology, Faculty of Veterinary Medicine, University of Life Sciences Lublin, 20-950 Lublin, Poland
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/ Piotr Siermontowski
  • Department of Maritime and Hyperbaric Medicine, Military Institute of Medicine, 81-103 Gdynia, Poland
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/ Jacek Mendocha / Marcin Orłowski / Katarzyna Van Damme-Ostapowicz
  • Department of Integrated Medical Care Medical University of Bialystok, 15-096 Bialystok, Poland
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Published Online: 2014-03-25 | DOI: https://doi.org/10.2478/bvip-2014-0020


The aim of this study was to develop and examine a model of apoptosis and necrosis of hepatocytes induced by a damaging factor - adriamycin, correlating time after its administration with cell death type, and to investigate the localisation within the liver acinus of hepatocytes dying in these two ways. The results obtained in the present and previous studies were compared in order to make a map of cell death localisation in the liver acinus, showing the effect of time in action and dose of adriamycin. The experiment was performed on 32 female Wistar rats, divided into four groups: I and II - experimental, and III and IV - control. Adriamycin (3 mg/kg b.w.) was administered intraperitoneally to rats in groups I and II, and the rats were decapitated after four (group I) and eight (group II) weeks. Animals in control groups III and IV were given 0.5 mL of 0.9% NaCl solution, and decapitated after four and eight weeks respectively. Sections of the liver were examined with a three-stage immunohistochemical method. This method allowed to examine hepatocytes qualitatively and quantitatively for the presence of proteins involved in three types of apoptosis: induced by the mitochondrial pathway (caspase 3, 9), the intrinsic pathway related to endoplasmic reticulum stress (caspase 3, 12), and the extrinsic pathway (caspase 3, 8). One of the inflammatory markers, caspase 1, was also examined. The zonal localisation of all three types of apoptosis was assessed in the liver tissue. More oxidated hepatocytes indicated only signs of the internal mitochondrial pathway, whereas less oxidated hepatocytes induced the internal reticular pathway and the external apoptotic pathway. The period between adriamycin administration and hepatic cell investigation was a main factor of the process. A longer period post insult resulted in a more pronounced effect of the activation of apoptosis. Sections explored eight weeks after treatment with different doses of the drug (3 and 5 mg/kg in the previous study) showed a similar intensity of apoptosis.

Keywords: rats; liver acinus; adriamycin; apoptosis; necrosis.


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About the article

Published Online: 2014-03-25

Published in Print: 2014-03-01

Citation Information: Bulletin of the Veterinary Institute in Pulawy, Volume 58, Issue 1, Pages 125–133, ISSN (Online) 2300-3235, DOI: https://doi.org/10.2478/bvip-2014-0020.

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