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Bulletin of the Veterinary Institute in Pulawy

The Journal of National Veterinary Research Institute in Pulawy

4 Issues per year


IMPACT FACTOR 2016: 0.462

SCImago Journal Rank (SJR) 2016: 0.235
Source Normalized Impact per Paper (SNIP) 2016: 0.364

Open Access
Online
ISSN
2300-3235
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Transcriptomic profiling of peripheral blood nucleated cells in dogs with and without clinical signs of chronic mitral valve disease

Magdalena Garncarz
  • Corresponding author
  • Department of Veterinary Diagnostics and Pathology, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, 02-787 Warsaw, Poland
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  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Magdalena Hulanicka
  • Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, 02-787 Warsaw, Poland
  • Other articles by this author:
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/ Marta Parzeniecka-Jaworska
  • Corresponding author
  • Department of Veterinary Diagnostics and Pathology, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, 02-787 Warsaw, Poland
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  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Jacek Garncarz / Michał Jank
  • Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, 02-787 Warsaw, Poland
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Published Online: 2014-03-25 | DOI: https://doi.org/10.2478/bvip-2014-0021

Abstract

The aim of the study was to demonstrate differences in the gene expression of signalling pathways between healthy dogs and dogs with chronic mitral valve disease in different heart failure groups. Blood samples were collected from 49 dogs of various breeds between 1.4 and 15.2 years of age. Isolated RNA samples were analysed for quality and integrity and the gene expression profile was determined. The study demonstrated that nucleated cells from peripheral blood can be used to assess the status of heart failure in dogs. Furthermore, significant differences in the expression of the genes were noticed between healthy dogs and dogs with clinical signs of chronic mitral valve disease. This is a preliminary non-invasive study showing the feasibility of genetic testing from peripheral blood nucleated cells, which at the same time has made it possible to set the future directions of genetic studies in clinical cases of canine chronic mitral valve disease.

Keywords: dog; transcriptomic profiling; gene expression; valve disease.

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About the article

Published Online: 2014-03-25

Published in Print: 2014-03-01


Citation Information: Bulletin of the Veterinary Institute in Pulawy, ISSN (Online) 2300-3235, DOI: https://doi.org/10.2478/bvip-2014-0021.

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© 2013. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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