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Bulletin of the Veterinary Institute in Pulawy

The Journal of National Veterinary Research Institute in Pulawy

4 Issues per year


IMPACT FACTOR 2016: 0.462

SCImago Journal Rank (SJR) 2016: 0.235
Source Normalized Impact per Paper (SNIP) 2016: 0.364

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Online
ISSN
2300-3235
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Comparative identification of Candida species isolated from animals using phenotypic and PCR-RFLP methods

George Cosmin Nadăş / Zsuzsa Kalmár / Marian Aurel Taulescu
  • Department of Pathology, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 400372 Cluj-Napoca, Romania
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Flore Chirilă / Cosmina Maria Bouari
  • Department of Pathology, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 400372 Cluj-Napoca, Romania
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Sorin Răpuntean / Pompei Bolfa
  • Department of Pathology, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 400372 Cluj-Napoca, Romania
  • Department of Biomedical Sciences, Ross University School of Veterinary Medicine Basseterre, P.O. Box 334, Basseterre, St. Kitts, West Indies
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/ Nicodim Iosif Fiţ
Published Online: 2014-06-17 | DOI: https://doi.org/10.2478/bvip-2014-0032

Abstract

The aim of this study was to identify 58 Candida sp. strains isolated from animals using the Chromatic Candida test, the API 20 C AUX system, and polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP). The Chromatic Candida test was able to identify only C. albicans and C. krusei. The API 20 C AUX system and PCR-RFLP had similar specificity for the identification of Candida strains. In case of both methods, Candida albicans was the most frequently isolated species - 22 (37.93%) strains, followed by Candida krusei - 17 (29.31%) strains, Candida famata - 10 (17.24%) strains, Candida parapsilosis - five (8.62%) strains, and Candida kefyr - four (6.89%) strains. PCR-RFLP represents a reliable, quick and relatively inexpensive genotyping method, recommended for rapid identification of Candida spp.

Keywords: Candida; identification; PCR-RFLP; Chromatic Candida; test API 20 C AUX system; Romania.

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About the article

Received: 2013-06-17

Accepted: 2014-06-02

Published Online: 2014-06-17

Published in Print: 2014-06-01


Citation Information: Bulletin of the Veterinary Institute in Pulawy, ISSN (Online) 2300-3235, DOI: https://doi.org/10.2478/bvip-2014-0032.

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© National Veterinary Research Institute in Pulawy. This article is distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. BY-NC-ND 3.0

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