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Bulletin of the Veterinary Institute in Pulawy

The Journal of National Veterinary Research Institute in Pulawy

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2300-3235
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Cytological evaluation of inflammation of the uterus and influence of endometritis on selected reproductive parameters in dairy cows

Piotr Brodzki
  • Department and Clinic of Reproduction, University of Life Sciences in Lublin, 20-612 Lublin, Poland
  • Email:
/ Krzysztof Niemczuk
  • National Veterinary Research Institute, 24-100 Pulawy, Poland
/ Krzysztof Kostro
  • Department of Epizootiology and Clinic of Infectious Diseases
/ Adam Brodzki
  • Department and Clinic of Animal Surgery
/ Łukasz Kurek
  • Department and Clinic of Internal Medicine, University of Life Sciences in Lublin, 20-612 Lublin, Poland
/ Jan Marczuk
  • Department and Clinic of Internal Medicine, University of Life Sciences in Lublin, 20-612 Lublin, Poland
Published Online: 2014-06-17 | DOI: https://doi.org/10.2478/bvip-2014-0035

Abstract

There were two aims of this study. One was to evaluate the postpartum state of the reproductive system in cows based on ultrasonography, bacterial culture, and cytological examination of the uterus. The other was to determine whether postpartum endometritis affects the subsequent state of the endometrium, and, in consequence, selected reproductive parameters in cows. The study was conducted on 60 cows: the experimental group of 30 cows with endometritis, and 30 cows free of uterine inflammation (control). The percentage of leukocytes in both groups was similar only on day 5 of postpartum. In all subsequent tests (26, 40, 61 d postpartum), the percentage of leukocytes in the experimental group was statistically significantly higher than in the control (P < 0.001), both in samples collected with a brush and in lavage samples. Involution of the uterus in the experimental group was also slower (P < 0.001). The analysed reproductive parameters were markedly less favourable in the experimental group than in the control. The study showed that postpartum inflammation of the uterus can persist for a long time in the form of endometritis, causing substantial deterioration of reproductive parameters in cows. The authors suggest that cytological evaluation of the uterus, preferably using a brush, should be performed as soon as possible after parturition, even before day 21, up to which time puerperal metritis may still persist. Evaluation of the inflammatory process based on the number of leukocytes and the quality of endometrial cells is important.

Keywords: cows; metritis; endometritis; endometrial cytology; reproductive parameters.

References

  • 1. Barański W., Podhalicz-Dzięgielewska M., Zduńczyk S., Janowski T.: The diagnosis and prevalence of subclinical endometritis in cows evaluated by different cytologic thresholds. Theriogeneology 2012, 78, 1939-1947.

  • 2. Barlund C.S., Carruthers T.D., Waldner C.L., Palmer C.W.: A comparison of diagnostic techniques for postpartum endometritis in dairy cattle. Theriogenology 2008, 69, 714-723. [Web of Science]

  • 3. Bisinotto R.S., Greco L.F., Ribeiro E.S., Martinez N., Lima F.S., Staples C.R., Thather W.W., Santos J.E.P.: Influences of nutrition and metabolism on fertility of dairy cows. Anim Reprod Sci. 2012, 9, 260-272.

  • 4. Čengić B., Varatanović N., Mutevelić T., Katica A., Mlačo N., Ćutuk A.: Normal and abnormal uterine involution in cows monitored by ultrasound. Biotechnology in Animal Husbandry 2012, 28, 205-217.

  • 5. Chapwanya A., Meade K.G., Narciandi F., Stanley P., Mee J.F., Doherty M.L., Callanan J.J., Farrelly C.O.: Endometrial biopsy: a valuable clinical and research tool in bovine reproduction. Theriogenology 2010, 73, 988-994. [Web of Science]

  • 6. Dubuc J., Duffield T.F., Leslie K.E., Walton J.S., LeBlanc S.J.: Definitions and diagnosis of postpartum endometritis in dairy cows. J Dairy Sci 2010, 93, 5225-5233. [Web of Science]

  • 7. Földi J., Kulcsár M., Pécsia A., Huyghe B., de Sa C., Lohuis J.A., Cox P., Huszenica G.: Bacterial complications of postpartum uterine involution in catle. Anim Reprod Sci 2006, 96, 265-281.

  • 8. Galvão K.N.: Postpartum uterine diseases in dairy cows. Anim Reprod Sci 2012, 9, 290-296.

  • 9. Garoussi M.T., Sasani F., Hovareshti P.: The histopatological survey of tissue in Holstein dairy cows with or without recorded reproductive disorders. Iranian J Vet Sci and Tech 2010, 2, 101-108.

  • 10. Gautam G., Nako T., Koike K., Long S.T., Yusuf M., Ranasinghe R.M., Hayashi A.: (2010) Spontaneous recovery or persistence of postpartum endometritis and risk factors for its persistence in Holstein cows. Theriogenology 2010, 152, 411-416. [Web of Science]

  • 11. Gilbert R.O.: The effects of endometritis on the establishment of pregnancy in cattle reproduction. Fertility and Development 2011, 24, 252-257. [Web of Science]

  • 12. Hansen P.J.: Regulation of uterine immune function by progesterone - lessons from the sheep. J Reprod Immun 1998, 40, 63-70.

  • 13. Herath S., Lilly S.T., Fischer D.P., Williams E.J., Dobson H., Bryant C.E., Sheldon I.M.: Bacterial lipopolysaccharide induces an endocrine switch from prostaglandin F2 alpha to prostaglandin E2 in bovine endometrium. Endocrinology 2009, 150, 1912-1920. [Web of Science]

  • 14. Kasimanickam R., Duffield T.F., Foster R.A., Gartley C.J., Leslie K.E., Walton J.S., Johnson W.H.: Endometrial cytology and ultrasonografy for the detection of subclinical endometritis in postpartum dairy cows. Theriogenology 2004, 62, 9-23.

  • 15. Kasimanickam R., Duffield T.F., Foster R.A., Gartley C.J., Leslie K.E., Walton J.S., Johnson W.H.: A comparison of the cytobrush and uterine lavage techniques to evaluate endometrial cytology in clinically normal postpartum dairy cows. Can Vet J 2005, 46, 255-259.

  • 16. LeBlanc S.J., Duffield T.F., Leslie K.E., Bateman K.G., Keefe G.P., Walton J.S., Johnson W.H.: Defining and diagnosing postpartum clinical endometritis and its impact on reproductive performance in dairy cows. J Dairy Sci 2002, 85, 2223-2236.

  • 17. LeBlanc S.J.: Postpartum uterine disease and dairy herd reproductive performance: A review. Vet J 2008, 176, 102-114.

  • 18. LeBlanc S.J.: Interactions of metabolism, inflammation, and reproductive tract health in the postpartum period in dairy cattle. Reprod Dom Anim 2012, 47, 18-30. [Web of Science] [Crossref]

  • 19. Leutert C., Suthar V., Heuwieser W.: Evaluation of transrectal examination of cervical diameter by palpation in dairy cows. J Dairy Sci 2013, 96, 1063-1070. [Web of Science]

  • 20. Lewis G.S.: Steroidal regulation of uterine immune defenses. Anim Reprod Sci 2004, 82, 281-294.

  • 21. Lincke A., Drillich M., Heuwieser W.: Subclinical endometritis in dairy cattle and its effect on fertility-a review of recent publications. Berl Munch Tierarztl Wochenschr 2007, 120, 245-250.

  • 22. Lόpez-Gatius F., Labernia J., Santolaria P., Lόpez-Bejar M., Rutllant J.: Effect of reproductive disorders previous to conception on pregnancy attrition in dairy cows. Theriogenology 1996, 46, 643-648.

  • 23. Melendez P., McHale J., Bartolome J., Archbald L.F., Donovan G.A.: Uterine involution and fertility of holstein cows subsequent to early postpartum PGF2α treatment for acute puerperal metritis. J Dairy Sci 2004, 87, 3238-3246.

  • 24. Plöntzke J., Madoz L.V., De la Sota R.L., Drillich M., Heuwieser W.: Subclinical endometritis and its impact on reproductive performance in grazing dairy cattle in Argentina. Anim Reprod Sci 2010, 122, 52-57. [Web of Science]

  • 25. Potter T.J., Guitian J., Fishwick O.J.G., Sheldon M.: Risk factors for clinical endometritis in postpartum dairy cattle. Theriogenology 2010, 74, 127-134. [Web of Science]

  • 26. Prieto M., Barrio M., Quintela L.A., Perez-Martin C.C., Becerra J.J., Vigo M., Diaz C., Cainzos J., Prieto A., Fernandez F.I., Martinez D., Herradon P.G.: Validation of simple method for the interpretation of uterine cytology in cows. Veterinarni Medicina 2012, 57, 360-363.

  • 27. Sheldon I.M., Noakes D.E., Rycroff A.N., Pfeiffer D.U., Dobson H.: Influence of uterine bacterial contamination after parturition on ovarian dominant follicle selection and follicle growth and function in cattle. Vet Rec 2002, 123, 837-845.

  • 28. Sheldon I.M., Levis G.S., LeBlanc S., Gilbert R.O.: Defining postpartum uterine disease in cattle. Theriogenology 2006, 65, 1516-1530.

  • 29. Sheldon M., Cronin J., Goetze L., Donofrio G., Schuberth H.J.: Defining postpartum uterine disease and the mechanisms of infection and immunity in the female reproductive tract in cattle. Biol Reprod 2009, 81, 1026-1032. [Web of Science]

  • 30. Sheldon I.M., Price S.B., Cronin J., Gilbert R.O., Gadsby J.E.: Mechanisms of infertility associated with clinical and subclinical endometritis in high producing dairy cattle. Reprod Domest Anim 2009, 44, 1-9. [Web of Science]

  • 31. Wathes D.C.: Mechanisms linking metabolic status and disease with reproductive outcome in the dairy cow. Reprod Dom Anim 2012, 47, 304-312. [Web of Science]

About the article

Received: 2013-10-21

Accepted: 2014-06-03

Published Online: 2014-06-17

Published in Print: 2014-06-01



Citation Information: Bulletin of the Veterinary Institute in Pulawy, ISSN (Online) 2300-3235, DOI: https://doi.org/10.2478/bvip-2014-0035. Export Citation

© National Veterinary Research Institute in Pulawy. This article is distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY-NC-ND 3.0)

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