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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Greaves, Ronda / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter


IMPACT FACTOR 2017: 3.556

CiteScore 2018: 2.44

SCImago Journal Rank (SJR) 2018: 1.191
Source Normalized Impact per Paper (SNIP) 2018: 1.205

Online
ISSN
1437-4331
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Volume 36, Issue 5

Issues

Quantitation and Enzymatic Activity of the Alkaline Protease from Pseudomonas aeruginosa in Culture Supernatants from Clinical Strains

Dominique Louis / Pierre Sorlier / Jean Wallach
Published Online: 2005-06-01 | DOI: https://doi.org/10.1515/CCLM.1998.050

Abstract

Alkaline protease from Pseudomonas aeruginosa (E.C. 3.4.24.40), also called pseudomonal serralysin, plays an important role in the infection bacterial process. The alkaline protease concentration and activity in the culture supernatants of 23 Pseudomonas aeruginosa strains from patients suffering from cystic fibrosis were determined by using an ELISA procedure and a specific enzymatic activity test. No correlation was demonstrated between the activity and the concentration of alkaline protease. This could be explained by the presence of an inactive precursor and a partial hydrolysis of the enzyme, confirmed by SDS-PAGE and Western blotting in the supernatants. The activity test is the only one allowing a quantification of the presence of an active form of alkaline protease.

About the article

Published Online: 2005-06-01

Published in Print: 1998-05-01


Citation Information: Clinical Chemistry and Laboratory Medicine, Volume 36, Issue 5, Pages 295–298, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.1998.050.

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