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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

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Online
ISSN
1437-4331
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Volume 36, Issue 6 (Jun 1998)

Issues

Peptide Models of Immunological Recognition: Paratope Dissection by Multiple Peptide Synthesis

Daniel Laune / Bernard Pau / Claude Granier
Published Online: 2005-06-01 | DOI: https://doi.org/10.1515/CCLM.1998.062

Abstract

New approaches to obtain information about the residues of the antibody paratope involved in the interaction with antigen would be very useful to help perform rational mutagenesis of antibodies for improving sensitivity or selectivity in immunoassays. We have evaluated the possibility of dissecting the antibody paratope into large sets of short (12 residues) overlapping peptides to determine the contribution of each peptide to antigen binding. Our results show that the systematic analysis of the antigen-binding properties of heavy chain variable segment and light chain variable segment derived peptides of HyHEL-5, a model anti-lysozyme antibody, can be an experimental approach to the selection of paratope-derived peptides with antigen-binding activity. Detailed analysis of the contribution of each residue from each binding peptide permitted the identification of residues contributing to antigen binding. Of the 38 residues we identified, 22 corresponded to residues that had been shown by X-ray crystallography to be at the interface between HyHEL-5 and lysozyme. The peptide analysis we have developed is thus a way to map the subset of residues from the antibody paratope involved in antigen recognition. The same peptide approach was used to map the idiotope that an anti-idiotypic antibody recognized in the paratope of its cognate antibody.

About the article

Published Online: 2005-06-01

Published in Print: 1998-06-01


Citation Information: Clinical Chemistry and Laboratory Medicine, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.1998.062. Export Citation

Citing Articles

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[1]
Pascale Cohen, Daniel Laune, Isabelle Teulon, Thérèse Combes, Martine Pugnière, Gabriel Badouaille, Claude Granier, Jean Claude Mani, and Dominique Simon
Journal of Immunological Methods, 2001, Volume 254, Number 1-2, Page 147
[2]
Cédric Bès, Laurence Briant-Longuet, Martine Cerruti, Piergiuseppe DeBerardinis, Gérard Devauchelle, Christian Devaux, Claude Granier, and Thierry Chardès
FEBS Letters, 2001, Volume 508, Number 1, Page 67

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