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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

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Analysis of Clonality in T-Lymphoproliferative Diseases by Multiplex PCR

Renata Zadro / Mirna Sučić / Igor Aurer / Jasminka Metelko-Kovačević / Boris Labar / Ana Stavljenić Rukavina
Published Online: 2005-06-01 | DOI: https://doi.org/10.1515/CCLM.1998.112


Distinction between benign and malignant T-cell lymphoproliferative diseases can be difficult using morphological criteria. Using multiplex polymerase chain reaction system we have tested a series of patients with various lymphoproliferative disorders to detect clonal T-lymphocyte populations.

Results show that clonal amplification products were obtained from all 10 patients with T-cell lymphoproliferative disorders while the amplification of DNA samples from B-cell neoplasms and normal individuals revealed polyclonal amplification products.

By splitting the multiplex primer mix, the patient specific T-cell receptor γ rearrangement was determined: five out of ten patients showed the exclusive presence of a single T-cell receptor γ gene rearrangement. Three patients exhibited two rearranged T-cell receptor γ genes, while in two patients positive reactions were obtained with three pairs of primers for variable and joining segments.

Molecular analysis of rearranged T-cell receptor genes by multiplex polymerase chain reaction represents a useful and rapid tool for confirming diagnosis, to determine the extent of disease and to monitor the response to therapy.

Published Online: 2005-06-01

Published in Print: 1998-08-01

Citation Information: Clinical Chemistry and Laboratory Medicine. Volume 36, Issue 8, Pages 637–639, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.1998.112, June 2005

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