Clinical Chemistry and Laboratory Medicine (CCLM)
Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)
Editor-in-Chief: Plebani, Mario
Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.
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Evaluation of a Direct α-Amylase Assay Using 2-Chloro-4-nitrophenyl-α-D-maltotrioside
Citation Information: Clinical Chemistry and Laboratory Medicine. Volume 37, Issue 11-12, Pages 1053–1062, ISSN (Print) 1434-6621, DOI: 10.1515/CCLM.1999.154, June 2005
- Published Online:
We present the adaptation of an IFCC method for α-amylase using 2-chloro-4-nitro-phenyl-α-D-maltotrioside as substrate (1) suited for routine work at 37℃. In the assay, a constant proportion of substrate, i. e. 92%, is directly converted to 2-chloro-4-nitrophenol and maltotriose. The method is based on multi- and univariate optimization leading to following measurement conditions: substrate, 2.25 mmol/l; chloride, 310 mmol/l; calcium 5.0 mmol/l; 4-morpholinoethanesulphonic acid, 50 mmol/l; pH 6.28. The assay may be carried out manually or by mechanized procedures, with substrate or sample start, and it shows these analytical properties in measuring amylase activity of sera: no lag phase, detection limit 2.9 U/l, linear range ≤820 U/l (for 300 s) or ≤1450 U/l (for 120 s of measurement), and total manual imprecision 3.2% (CV) at 46 U/l. Bilirubin ≤630 μmol/l, haemoglobin ≤6 g/l, triacylglycerols ≤30 mmol/l, heparin ≤100 kU/l, and glucose ≤120 mmol/l do not interfere. For adults, we established a preliminary 0.95-reference interval of 30–90 U/l not dependent on sex or age. A close association with the IFCC method demonstrates the reliable transfer of its measurement conditions to a robust routine method with minimal changes.
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