Jump to ContentJump to Main Navigation
Show Summary Details
In This Section

Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

12 Issues per year

IMPACT FACTOR 2016: 3.432

CiteScore 2016: 2.21

SCImago Journal Rank (SJR) 2015: 0.873
Source Normalized Impact per Paper (SNIP) 2015: 0.982

See all formats and pricing
In This Section
Volume 38, Issue 2 (Feb 2000)


Unravelling Genetic Data by Arrayed Primer Extension

Neeme Tõnisson / Ants Kurg / Krista Kaasik / Elin Lõhmussaar / Andres Metspalu
Published Online: 2005-06-01 | DOI: https://doi.org/10.1515/CCLM.2000.025


We have developed a method for arrayed primer extension (APEX) on an oligonucleotide microchip together with the 4-color fluoresence imaging equipment and supporting software, that allows analysis of the DNA sequence and changes in it. Mutation analysis of BRCA1 gene and single nucleotide polymorphism (SNP) chip for genotyping were used as a model system. Chip surface chemistry, template preparation and APEX reaction conditions were optimised and the assay is ready to be implemented in variety of DNA analysis from SNP testing to DNA resequencing.

About the article

Published Online: 2005-06-01

Published in Print: 2000-02-05

Citation Information: Clinical Chemistry and Laboratory Medicine, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2000.025. Export Citation

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

Galuh D N Astuti, Mette Bertelsen, Markus N Preising, Muhammad Ajmal, Birgit Lorenz, Sultana M H Faradz, Raheel Qamar, Rob W J Collin, Thomas Rosenberg, and Frans P M Cremers
European Journal of Human Genetics, 2015
Sergei A. Aksyonov, Michael Bittner, Linda B. Bloom, Linda J. Reha-Krantz, Ian R. Gould, Mark A. Hayes, Urban A. Kiernan, Eric E. Niederkofler, Vincent Pizziconi, Raul S. Rivera, Daniel J.B. Williams, and Peter Williams
Analytical Biochemistry, 2006, Volume 348, Number 1, Page 127
Li Gao and Zuhong Lu
Biochemical and Biophysical Research Communications, 2009, Volume 387, Number 3, Page 421
Iris Schrijver, Maigi Külm, Phyllis I. Gardner, Eugene P. Pergament, and Morris B. Fiddler
The Journal of Molecular Diagnostics, 2007, Volume 9, Number 2, Page 228
Niels G.F. Klitø, Qihua Tan, Mette Nyegaard, Klaus Brusgaard, Mads Thomassen, Charlotte Skouboe, Jesper Dahlgaard, and Torben A. Kruse
Genetic Testing, 2007, Volume 11, Number 2, Page 160
Eugene Pergament, Christina Alamillo, Katrin Sak, and Morris Fiddler
Prenatal Diagnosis, 2011, Volume 31, Number 3, Page 307
Pui-Yan Kwok
Annual Review of Genomics and Human Genetics, 2001, Volume 2, Number 1, Page 235
Christos Shammas, Thessalia Papasavva, Xenia Felekis, Christos Christophorou, Hanno Roomere, Jan Traeger Synodinos, Emmanuel Kanavakis, Mohammed El-Khateeb, Hanan Hamamy, Tamara Mahmoud, Mohammad Shboul, Amal El Beshlawy, Dvora Filon, Ibtessam R. Hussein, Renzo Galanello, Giovanni Romeo, and Marina Kleanthous
Clinical Chemistry and Laboratory Medicine, 2010, Volume 48, Number 12
Lars Allan Larsen, Michael Christiansen, Jens Vuust, and Paal Skytt Andersen
Pharmacogenomics, 2001, Volume 2, Number 4, Page 387
L Gojová, E Jansová, M Külm, S Pouchlá, and L Kozák
Clinical Genetics, 2008, Volume 73, Number 5, Page 441
Barbara Franke, Sita H.H.M. Vermeulen, Regine P.M. Steegers-Theunissen, Marieke J. Coenen, Mascha M.V.A.P. Schijvenaars, Hans Scheffer, Martin den Heijer, and Henk J. Blom
Birth Defects Research Part A: Clinical and Molecular Teratology, 2009, Volume 85, Number 3, Page 216
Pagona Lagiou, Christina Georgila, Ploumitsa Minaki, Wolfgang Ahrens, Hermann Pohlabeln, Simone Benhamou, Christine Bouchardy, Alena Slamova, Miriam Schejbalova, Franco Merletti, Lorenzo Richiardi, Kristina Kjaerheim, Antonio Agudo, Xavier Castellsague, Tatiana V. Macfarlane, Gary J. Macfarlane, Renato Talamini, Luigi Barzan, Cristina Canova, Lorenzo Simonato, Ray Lowry, David I. Conway, Patricia A. McKinney, Ariana Znaor, Bernard E. McCartan, Claire Healy, Mari Nelis, Andres Metspalu, Manuela Marron, Mia Hashibe, and Paul J. Brennan
European Journal of Cancer Prevention, 2009, Volume 18, Number 1, Page 76
T. Majtán, G. Bukovská, and J. Timko
Folia Microbiologica, 2004, Volume 49, Number 6, Page 635

Comments (0)

Please log in or register to comment.
Log in