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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Greaves, Ronda / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter

IMPACT FACTOR 2018: 3.638

CiteScore 2018: 2.44

SCImago Journal Rank (SJR) 2018: 1.191
Source Normalized Impact per Paper (SNIP) 2018: 1.205

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Volume 38, Issue 3


On Accuracy and Precision of a HPLC Method for Measurement of Urine Porphyrin Concentrations

Floris M.J. Zuijderhoudt / Stephan G.A. Koehorst / Willem E. Kluitenberg / Jenneke Dorresteijn-de Bok
Published Online: 2005-06-01 | DOI: https://doi.org/10.1515/CCLM.2000.033


We studied the accuracy and precision of a HPLC method for determination of porphyrins in urine. A commercial standard solution appeared to contain less porphyrins than indicated by the manufacturer, since calibration resulted in lower concentrations of uroporphyrin and coproporphyrin: 16% and 8%, respectively. Coefficients of variation for the measurement of uro-, hepta-, copro I and copro III porphyrins in samples of patients with and without porphyria were often much less than 15%. Comparison of measurements with and without calibrated standards revealed differences for uroporphyrin and coproporphyrin of 27% and 5%, respectively. Recovery of added uroporphyrin and coproporphyrin was 99%. The main cause of the variability in test results was apparently the improperly calibrated standard solutions. The precision of porphyrin measurements was not influenced by the type of porphyria.

About the article

Published Online: 2005-06-01

Published in Print: 2000-03-25

Citation Information: Clinical Chemistry and Laboratory Medicine, Volume 38, Issue 3, Pages 227–230, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2000.033.

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