Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Greaves, Ronda / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter

IMPACT FACTOR 2018: 3.638

CiteScore 2018: 2.44

SCImago Journal Rank (SJR) 2018: 1.191
Source Normalized Impact per Paper (SNIP) 2018: 1.205

See all formats and pricing
More options …
Volume 38, Issue 3


Quality Control of Coated Antibodies: New, Rapid Determination of Binding Affinity

Rémy Ricoux / Bénédicte Chazaud / Jean-Pierre Tresca / Michel Pontet
Published Online: 2005-06-01 | DOI: https://doi.org/10.1515/CCLM.2000.035


A procedure is described for the determination of the affinity constant between a fluid-phase biotinylated antigen and a solid-phase monoclonal antibody. This procedure allows evaluation of the efficiency of an antibody as a coated tool for an immunoassay. For this purpose, the biotinylation of the antigen and its further quantitative measurement by streptavidin-peroxidase led to a single reversible interaction, the binding affinity of which greatly determines the quality of the assay. The free and bound fractions of the biotinylated antigen were obtained in wells coated with a low level of immobilized antibodies. At the equilibrium state, the free antigen present in the supernatant of these wells was further transferred to high level antibody coated wells which captured all the free antigen molecules. These molecules were quantified using a standard curve established with known concentrations of biotinylated antigen, also incubated in wells coated with the high level of antibody.

About the article

Published Online: 2005-06-01

Published in Print: 2000-03-25

Citation Information: Clinical Chemistry and Laboratory Medicine, Volume 38, Issue 3, Pages 239–243, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2000.035.

Export Citation

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

Azza M. Abdel-Aty, Mohamed Belal Hamed, Abdul Aziz M. Gad, Amr E. El-Hakim, and Saleh A. Mohamed
Veterinary World, 2018, Volume 11, Number 10, Page 1364
Nahla A. Hussein, Yasser E. Shahein, Amr E. El-Hakim, Amira M. Abouelella, Rasha A. Guneidy, and Ragaa R. Hamed
International Journal of Biological Macromolecules, 2014, Volume 67, Page 7
Yasser E. Shahein, Mohamed T. Abd El-Rahim, Nahla A. Hussein, Ragaa R. Hamed, Amr E. El-Hakim, and Maged M. Barakat
International Journal of Biological Macromolecules, 2010, Volume 47, Number 5, Page 614

Comments (0)

Please log in or register to comment.
Log in