Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Greaves, Ronda / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter

IMPACT FACTOR 2018: 3.638

CiteScore 2018: 2.44

SCImago Journal Rank (SJR) 2018: 1.191
Source Normalized Impact per Paper (SNIP) 2018: 1.205

See all formats and pricing
More options …
Volume 38, Issue 4


Influence of in vivo Hemoglobin Carbamylation on HbA1c Measurements by Various Methods

Abdelhalim Chachou / Christine Randoux / Hervé Millart / Jacques Chanard / Philippe Gillery
Published Online: 2005-06-01 | DOI: https://doi.org/10.1515/CCLM.2000.046


Increased carbamylated hemoglobin formed in erythrocytes during uremia may interfere with Hba1c assays, but few studies compared directly both parameters. We measured carbamylated hemoglobin by HPLC in 45 non-diabetic uremic patients (16 with acute and two with chronic renal failure, 27 with transplant recipients) as 57.8 ± 22.3 μg carbamylvaline/g Hb (mean ± standard deviation) vs. 31.6 ± 5.1 in 15 controls (+83%, p < 0.001). In these samples, HbA1c was evaluated by three ion-exchange HPLC methods, 1: Diamat (BioRad), 2: A1c2.2 (Tosoh) and 3: HA8140 (Menarini), and one immunoassay method (Tinaquant II Roche). Whichever the method, mean HbA1c values obtained increased in patients with high (> 60 μg carbamylvaline/g Hb) vs. low (< 45) carbamylated hemoglobin values (+0.08 to 0.25% of total Hb), but differences were not significant. Minor peaks on the chromatograms were however increased in parallel to carbamylated hemoglobin. HbA1c values over 6% were found in 4, 1, 2 and 0 samples, with HPLC 1, 2, 3 and immunoassay, respectively. Fructosamine values were not significantly altered. Our results show that Hb adducts, whether due to carbamylation or to other chemical reactions, interfere to a variable extent with different HbA1c assay methods, and confirm that HbA1c values should be interpreted with caution in uremic patients.

About the article

Published Online: 2005-06-01

Published in Print: 2000-04-30

Citation Information: Clinical Chemistry and Laboratory Medicine, Volume 38, Issue 4, Pages 321–326, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2000.046.

Export Citation

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

David C Wheeler
The British Journal of Diabetes & Vascular Disease, 2002, Volume 2, Number 2, Page 77
Michael L. Granda, Pooja Amarapurkar, and Alessia Fornoni
Clinical Science, 2018, Volume 132, Number 11, Page 1085
Andrea Tesija Kuna, Kristina Dukic, Nora Nikolac Gabaj, Marijana Miler, Ines Vukasovic, Sanja Langer, Ana-Maria Simundic, and Nada Vrkic
Laboratory Medicine, 2018
Rajesh Rajput, Binayak Sinha, Sujoy Majumdar, M. Shunmugavelu, and Sarita Bajaj
Diabetes Research and Clinical Practice, 2017, Volume 127, Page 10
Jay S. Skyler, George L. Bakris, Ezio Bonifacio, Tamara Darsow, Robert H. Eckel, Leif Groop, Per-Henrik Groop, Yehuda Handelsman, Richard A. Insel, Chantal Mathieu, Allison T. McElvaine, Jerry P. Palmer, Alberto Pugliese, Desmond A. Schatz, Jay M. Sosenko, John P.H. Wilding, and Robert E. Ratner
Diabetes, 2017, Volume 66, Number 2, Page 241
Vlado Perkovic, Rajiv Agarwal, Paola Fioretto, Brenda R. Hemmelgarn, Adeera Levin, Merlin C. Thomas, Christoph Wanner, Bertram L. Kasiske, David C. Wheeler, Per-Henrik Groop, George L. Bakris, Mark E. Cooper, Dong-Wan Chae, Michael H. Davidson, Ian H. de Boer, Dick de Zeeuw, Alessia Fornoni, Luigi Gnudi, Charles A. Herzog, Adriana M. Hung, Tazeen Hasan Jafar, Meg Jardine, Vivekanand Jha, Linong Ji, Steven E. Kahn, Robyn G. Langham, Edgar V. Lerma, Ronald C.W. Ma, Hirofumi Makino, Michel Marre, Michael Mauer, Kaj Metsärinne, Robert G. Nelson, Roberto Pecoits-Filho, Carol A. Pollock, Mohan Rajapurkar, Peter Rossing, Ivan Rychlík, Kumar Sharma, Robert C. Stanton, Vladimír Tesař, Ilkka Tikkanen, Charlie R.V. Tomson, Robert D. Toto, Yusuke Tsukamoto, Katherine R. Tuttle, Takashi Wada, Winfred W. Williams, Hong Zhang, and Sophia Zoungas
Kidney International, 2016, Volume 90, Number 6, Page 1175
E. Oei, N. Samad, A. Visser, T. A. Chowdhury, and S. L. Fan
Diabetic Medicine, 2016, Volume 33, Number 9, Page e17
Anna Pieniazek, Joanna Brzeszczynska, Ilona Kruszynska, and Krzysztof Gwozdzinski
Free Radical Research, 2009, Volume 43, Number 10, Page 1008
Gabriela Cavagnolli, Ana Laura Pimentel, Priscila Aparecida Correa Freitas, Jorge Luiz Gross, and Joíza Lins Camargo
Clinica Chimica Acta, 2015, Volume 445, Page 107
P. Gillery
Annales Pharmaceutiques Françaises, 2014, Volume 72, Number 5, Page 330
M. Speeckaert, W. Van Biesen, J. Delanghe, R. Slingerland, A. Wiecek, J. Heaf, C. Drechsler, R. Lacatus, R. Vanholder, I. Nistor, H. Bilo, D. Bolignano, C. Couchoud, A. Covic, L. Coentrao, J. D. Sutter, C. Drechsler, L. Gnudi, D. Goldsmith, J. Heaf, O. Heimburger, K. Jager, H. Nacak, I. Nistor, M. Soler, C. Tomson, L. Vanhuffel, W. V. Biesen, S. V. Laecke, L. Weekers, and A. Wiecek
Nephrology Dialysis Transplantation, 2014, Volume 29, Number 12, Page 2167
Qing Li, Yi Ju, Ting Jin, Biqiong Pang, Jiade Deng, Tongxin Du, and Hong Wang
Clinical Biochemistry, 2014, Volume 47, Number 6, Page 481
P. Gillery
EMC - Biologie médicale, 2009, Volume 4, Number 3, Page 1
Randie R. Little, Curt L. Rohlfing, Alethea L. Tennill, Steven E. Hanson, Shawn Connolly, Trefor Higgins, Charles E. Wiedmeyer, Cas W. Weykamp, Richard Krause, and William Roberts
Clinica Chimica Acta, 2013, Volume 418, Page 73
Sema Genc, Beyhan Omer, Esra Aycan-Ustyol, Nurhan Ince, Fatih Bal, and Figen Gurdol
Journal of Clinical Laboratory Analysis, 2012, Volume 26, Number 6, Page 481
Dilbahar S. Mohar, Ailin Barseghian, Nezam Haider, Michael Domanski, and Jagat Narula
Medical Clinics of North America, 2012, Volume 96, Number 1, Page 57
Nathalie Mario and Elisabeth Lasnier
Revue Francophone des Laboratoires, 2006, Volume 2006, Number 382, Page 39
James K. Fleming
Clinical Biochemistry, 2007, Volume 40, Number 11, Page 822
Alessandro Terreni, Renata Paleari, Anna Caldini, Agostino Ognibene, Andrea Mosca, and Gianni Messeri
Clinical Biochemistry, 2003, Volume 36, Number 8, Page 607
Sabin Shurraw, Sumit R. Majumdar, Ravi Thadhani, Natasha Wiebe, and Marcello Tonelli
American Journal of Kidney Diseases, 2010, Volume 55, Number 5, Page 875
M. Martin, N. Leroy, V. Sulmont, and P. Gillery
Diabetes & Metabolism, 2010, Volume 36, Number 2, Page 158
Jean Szymezak, Nathalie Leroy, Emmanuelle Lavalard, and Philippe Gillery
Clinical Chemistry and Laboratory Medicine, 2008, Volume 46, Number 8
Jean Szymezak, Emmanuelle Lavalard, Michèle Martin, Nathalie Leroy, and Philippe Gillery
Clinical Chemistry and Laboratory Medicine, 2009, Volume 47, Number 5
Sudip Nanda, Surya Prakash Bhatt, John Pamula, Mohammad I. Arastu, and Shree Gopal Sharma
The American Journal of the Medical Sciences, 2008, Volume 335, Number 5, Page 382
S. Hadjadj, D. Coisne, G. Mauco, S. Ragot, F. Duengler, P. Sosner, F. Torremocha, D. Herpin, and R. Marechaud
Diabetic Medicine, 2004, Volume 21, Number 4, Page 305

Comments (0)

Please log in or register to comment.
Log in