Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

12 Issues per year

IMPACT FACTOR 2016: 3.432

CiteScore 2016: 2.21

SCImago Journal Rank (SJR) 2016: 1.000
Source Normalized Impact per Paper (SNIP) 2016: 1.112

See all formats and pricing
More options …
Volume 38, Issue 6


Effect of Short- and Long-Term Storage on Human Serum and Recombinant Apolipoprotein E Concentration

Françoise Schiele / Monique Vincent-Viry / Bernard Herbeth / Athanase Visvikis / Gérard Siest
Published Online: 2005-06-01 | DOI: https://doi.org/10.1515/CCLM.2000.077


In order to assess the short- and long-term stability of apolipoprotein (apo) E concentration in serum, we compared the apo E concentrations measured in fresh human serum samples with those determined after storage at +4°C, −20°C or −80°C. The serum apo E concentration was measured by immunoturbidimetry using an anti-human apo E polyclonal antibody from goats. One week storage at +4°C did not significantly affect the serum apo E concentration. At −20°C or −80°C no significant change in apo E concentration occurred during up to three months of storage. Moreover, the concentration of apo E was not modified after long-term storage of serum samples kept at −196°C in liquid nitrogen for up to four years. In addition, 15 freeze-thaw cycles, over a 3-week period, did not affect the apo E concentration in serum. A similar freezethaw procedure applied to purified human recombinant apo E showed that apo E2 isoform was the most stable in comparison with the apo E3 and apo E4 isoforms.

About the article

Published Online: 2005-06-01

Published in Print: 2000-06-11

Citation Information: Clinical Chemistry and Laboratory Medicine, Volume 38, Issue 6, Pages 525–528, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2000.077.

Export Citation

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

Allison Hubel, Ralf Spindler, and Amy P.N. Skubitz
Biopreservation and Biobanking, 2014, Volume 12, Number 3, Page 165
Gunter Kenis, Charlotte Teunissen, Raf De Jongh, Eugène Bosmans, Harry Steinbusch, and Michael Maes
Cytokine, 2002, Volume 19, Number 5, Page 228
E. O. Nardid, E. D. Rozanova, L. V. Tsymbal, A. V. Zinchenko, O. A. Nardid, and V. I. Grishchenko
Biophysics, 2009, Volume 54, Number 5, Page 621

Comments (0)

Please log in or register to comment.
Log in