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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

12 Issues per year


IMPACT FACTOR increased in 2015: 3.017
Rank 5 out of 30 in category Medical Laboratory Technology in the 2014 Thomson Reuters Journal Citation Report/Science Edition

SCImago Journal Rank (SJR) 2015: 0.873
Source Normalized Impact per Paper (SNIP) 2015: 0.982
Impact per Publication (IPP) 2015: 2.238

Online
ISSN
1437-4331
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Volume 38, Issue 8 (Aug 2000)

Issues

Automated Enzymatic Mitochondrial Antibody Assay for the Diagnosis of Primary Biliary Cirrhosis

Wayne A. Jensen / Jennifer A. Jois / Peter Murphy / Joseph De Giorgio / Belinda Brown / Merrill J. Rowley / Ian R. Mackay
Published Online: 2005-06-01 | DOI: https://doi.org/10.1515/CCLM.2000.107

Abstract

Primary biliary cirrhosis is a progressive autoimmune disease that affects middle aged women, resulting in liver cirrhosis. We describe here an automated enzymatic mitochondrial antibody assay adapted for performance on laboratory analysers for the serological diagnosis of primary biliary cirrhosis. This assay detects the characteristic autoantibody directed against the 74kDa E2 subunit of the pyruvate dehydrogenase complex.

Analysis of receiver operator characteristic curve data indicated that the automated enzymatic mitochondrial assay procedure discriminated clinically identified patients with primary biliary cirrhosis from normal subjects with a sensitivity of 83% and a specificity of 100%. This method compared favourably against a commercial ELISA method which had a sensitivity of 73% and a specificity of 100%. The automated enzymatic mitochondrial antibody assay is a high throughput assay of use for the routine diagnosis of patients with primary biliary cirrhosis with autoantibodies to the E2 subunit of the pyruvate dehydrogenase complex. The method is of potential value for economical and rapid screening to detect asymptomatic primary biliary cirrhosis in the at-risk segment of the population, namely middle aged women.

About the article

Published Online: 2005-06-01

Published in Print: 2000-08-21


Citation Information: Clinical Chemistry and Laboratory Medicine, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2000.107. Export Citation

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[1]
Danilo Villalta, Maria G. Mytilinaiou, Martina Elsner, Christian Hentschel, Juliane Cuccato, Valentina Somma, Peter Schierack, Dirk Roggenbuck, and Dimitrios P. Bogdanos
Clinica Chimica Acta, 2015
[2]
Ian R. Mackay
Best Practice & Research Clinical Gastroenterology, 2000, Volume 14, Number 4, Page 519
[3]
HIROAKI HAZAMA, KATSUHISA OMAGARI, JUN-ICHI MASUDA, KAZUO OHBA, HIDEKI KINOSHITA, ISAO MATSUO, HAJIME ISOMOTO, YOHEI MIZUTA, KUNIHIKO MURASE, IKUO MURATA, and SHIGERU KOHNO
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