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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Greaves, Ronda / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter

IMPACT FACTOR 2017: 3.556

CiteScore 2017: 2.34

SCImago Journal Rank (SJR) 2017: 1.114
Source Normalized Impact per Paper (SNIP) 2017: 1.188

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Volume 38, Issue 8


Comparison of Two Different Methods for Measurement of Phenylalanine in Dried Blood Spots

Adriana Rivero / Juan Antonio Allué / Ana Grijalba / Mercedes Palacios / Sergio García Merlo
Published Online: 2005-06-01 | DOI: https://doi.org/10.1515/CCLM.2000.110


Phenylketonuria is an inherited metabolic disorder caused by a defect in the hydroxylation of phenylalanine. Newborn screening is crucial for the diagnosis and treatment of this disease. A phenylalanine dehydrogenase-coupled enzymatic assay (Quantase) in microtiter plates for the screening of phenylketonuria was evaluated and compared with our routine method based on the modified fluorometric McCaman method. The test exhibited a linear calibration curve with a good slope as well as sufficient imprecision (< 10%), recovery (99.23 ± 4.86%) and limit of detection (54.5 μmol/l). One hundred and ninety dried blood spots were analysed by this enzymatic method and compared with McCaman's. Although Quantase (Teknovas, Bilbao, Spain) showed a phenylalanine mean level in dried blood spot 18.2 μmol/l higher than that obtained with our routine method, the agreement between both techniques was considered acceptable.

About the article

Published Online: 2005-06-01

Published in Print: 2000-08-21

Citation Information: Clinical Chemistry and Laboratory Medicine, Volume 38, Issue 8, Pages 773–776, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2000.110.

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