Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Greaves, Ronda / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter


IMPACT FACTOR 2018: 3.638

CiteScore 2018: 2.44

SCImago Journal Rank (SJR) 2018: 1.191
Source Normalized Impact per Paper (SNIP) 2018: 1.205

Online
ISSN
1437-4331
See all formats and pricing
More options …
Volume 38, Issue 9

Issues

Hypervariable Region 1 of Hepatitis C Virus Genome and Response to Interferon Therapy

Blaženka Grahovac / Jasna Bingulac-Popović / Boris Vucelić / Irena Hrstić / Rajko Ostojić / Vesna Dražić / Melita Balija / Damir Grgičević
Published Online: 2005-06-01 | DOI: https://doi.org/10.1515/CCLM.2000.132

Abstract

The relationship between the complexity of the hypervariable region 1 (HVR1) quasispecies of hepatitis C virus (HCV) and responsiveness to interferon-α (IFN) therapy was studied in patients with chronic hepatitis C. Twelve HCV-RNA-positive patients were treated daily with high dose IFN and ribavirin for 4 weeks, and then with IFN 3 MIU (Million International Units) TIW (three times per week) and ribavirin for 6 months. The HVR1 quasispecies complexity was analyzed by nested polymerase chain reaction-mediated single-strand conformation polymorphism (SSCP). The baseline HCV-RNA levels in the study group ranged from 106 to 107 copies/ml. All patients exhibited HCV genotype 1 b. Initial SSCP analysis revealed four (33.3%) patients with a low complexity pattern (SSCP bands ≤4) and eight (66.6%) patients with high complexity pattern (SSCP bands >4). After 4 weeks of IFN therapy, one patient became HCV negative, and among those remaining positive, the HCV-RNA levels decreased by 2 to 3 logs and the number of SSCP decreased by 2 to 3 bands per sample. After 6 months of IFN therapy, five (41.7%) patients became HCV-RNA-negative. Seven (58.3%) patients did not respond to IFN therapy with sustained viral load from 103 to 105 copies/ml, and high complexity SSCP patterns. Our data support the HVR quasispecies complexity to be an independent predictive factor for IFN responsiveness in patients infected with HCV.

About the article

Published Online: 2005-06-01

Published in Print: 2000-09-18


Citation Information: Clinical Chemistry and Laboratory Medicine, Volume 38, Issue 9, Pages 905–910, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2000.132.

Export Citation

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
E. Domingo, J. Sheldon, and C. Perales
Microbiology and Molecular Biology Reviews, 2012, Volume 76, Number 2, Page 159
[2]
Xin-Xin Zhang
World Journal of Gastroenterology, 2003, Volume 9, Number 5, Page 1003
[3]
Elizabeth Kenny-Walsh, Liam J. Fanning, Daniel Schmidt-Martin, and Orla Crosbie
Journal of General Virology, 2015, Volume 96, Number 8, Page 2145
[4]
Isabella Abbate, Oreste Lo Iacono, Rosa Di Stefano, Giuseppina Cappiello, Enrico Girardi, Roberta Longo, Donatella Ferraro, Giorgio Antonucci, Vito Di Marco, Mariacarmela Solmone, Antonio Craxı̀, Giuseppe Ippolito, and Maria R Capobianchi
Journal of Hepatology, 2004, Volume 40, Number 5, Page 831
[5]
Jason T. Blackard, Gang Ma, Jeffrey A. Welge, Christina M. Martin, Kenneth E. Sherman, Lynn E. Taylor, Kenneth H. Mayer, and Denise J. Jamieson
Journal of Medical Virology, 2012, Volume 84, Number 2, Page 242
[6]
Blazenka Grahovac, Jasna Bingulac-Popovic, Boris Vucelic, Irena Hrstic, Rajko Ostojic, Vesna Drazic, Melita Balija, and Damir Grgicevic
Journal of Clinical Virology, 2001, Volume 20, Number 1-2, Page 85
[7]
Kazuaki Chayama and C Nelson Hayes
Journal of Gastroenterology and Hepatology, 2011, Volume 26, Page 83
[8]
Javier Salmerón, Paloma Muñoz De Rueda, Ángela Ruiz-Extremera, Jorge Casado, Carlos Huertas, Maria Del Carmen Bernal, Luis Rodríguez, and Ángel Palacios
Digestive Diseases and Sciences, 2006, Volume 51, Number 5, Page 960

Comments (0)

Please log in or register to comment.
Log in