Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

12 Issues per year

IMPACT FACTOR 2016: 3.432

CiteScore 2016: 2.21

SCImago Journal Rank (SJR) 2016: 1.000
Source Normalized Impact per Paper (SNIP) 2016: 1.112

See all formats and pricing
More options …
Volume 39, Issue 3 (Apr 2001)


Simultaneous Detection of Multiple Proteins with an Array-Based Enzyme-Linked Immunosorbent Assay (ELISA) and Enhanced Chemiluminescence (ECL)

Ruo-Pan Huang
Published Online: 2005-06-01 | DOI: https://doi.org/10.1515/CCLM.2001.032


Protein arrays hold a promise in basic and clinical applications. As the first step to develop such array system, I used an array-based enzyme-linked immunosorbent assay (ELISA) and enhanced chemiluminescence (ECL) to demonstrate the feasibility of simultaneous detection of multiple proteins. In the direct ELISA system, different known immunoglobulin Gs (IgGs) were immobilized onto polyvinylidine difloride (PDVF) membrane through 96-well format Bio-Dot unit. The antigens were then individually and collectively detected by incubation of membranes with different antibodies coupled with ECL. In the sandwich ELISA system, the cytokine capture antibodies were immobilized onto PDVF membranes. The membranes were then incubated with single cytokine or a combination of different cytokines. The captured cytokines were detected by biotin-conjugated antibodies coupled with ECL system. Experiments demonstrated that multiple IgGs and cytokines could be simultaneously detected using this approach with high specificity and sensitivity. More importantly, cytokines from biological samples were detected using this approach, which can be used in any general laboratory setting without any sophisticated equipment. This concept could be extended to develop a protein-based array system.

About the article

Published Online: 2005-06-01

Published in Print: 2001-04-09

Citation Information: Clinical Chemistry and Laboratory Medicine, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2001.032.

Export Citation

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

Dieter Stoll, Jutta Bachmann, Markus F. Templin, and Thomas O. Joos
Drug Discovery Today: TARGETS, 2004, Volume 3, Number 1, Page 24
Akiyoshi Hoshino, Kouki Fujioka, Noriyoshi Manabe, Shun-ichi Yamaya, Yoji Goto, Masato Yasuhara, and Kenji Yamamoto
Microbiology and Immunology, 2005, Volume 49, Number 5, Page 461
Brian T. Cunningham and Richard C. Zangar
Journal of Biophotonics, 2012, Volume 5, Number 8-9, Page 617
Aimee S. Browne, Jie Yu, Ruo-Pan Huang, Antônio M.C. Francisco, Neil Sidell, and Robert N. Taylor
Fertility and Sterility, 2012, Volume 98, Number 3, Page 713
Stephen D. Mikolajczyk, Yong Song, James R. Wong, Robert S. Matson, and Harry G. Rittenhouse
Clinical Biochemistry, 2004, Volume 37, Number 7, Page 519
Ying Lin, Ruochun Huang, Nalini Santanam, Ya-Guang Liu, Sampath Parthasarathy, and Ruo-Pan Huang
Cancer Letters, 2002, Volume 187, Number 1-2, Page 17
Markus F Templin, Dieter Stoll, Monika Schrenk, Petra C Traub, Christian F Vöhringer, and Thomas O Joos
Trends in Biotechnology, 2002, Volume 20, Number 4, Page 160
Markus F. Templin, Dieter Stoll, Monika Schrenk, Petra C. Traub, Christian F. Vöhringer, and Thomas O. Joos
Drug Discovery Today, 2002, Volume 7, Number 15, Page 815
Thomas O Joos, Dieter Stoll, and Markus F Templin
Current Opinion in Chemical Biology, 2002, Volume 6, Number 1, Page 76
Ruo-Pan Huang
Expert Review of Proteomics, 2007, Volume 4, Number 2, Page 299
Richard C Zangar, Don S Daly, and Amanda M White
Expert Review of Proteomics, 2006, Volume 3, Number 1, Page 37
James F. Rusling, Challa V. Kumar, J. Silvio Gutkind, and Vyomesh Patel
The Analyst, 2010, Volume 135, Number 10, Page 2496
Bum Hwan Lee and Teruyuki Nagamune
Biotechnology and Bioprocess Engineering, 2004, Volume 9, Number 2, Page 69
Ruo-Pan Huang, Weimin Yang, Dongzi Yang, Lisa Flowers, Ira R Horowitz, Xuan Cao, and Ruochun Huang
Expert Opinion on Therapeutic Targets, 2005, Volume 9, Number 3, Page 601
Pieranna Chiarella and Vito Michele Fazio
Biotechnology Letters, 2008, Volume 30, Number 8, Page 1303
Tsunenori Matsunaga, Kajiro Yanagiguchi, Shizuka Yamada, Naoko Ohara, Takeshi Ikeda, and Yoshihiko Hayashi
Journal of Biomedical Materials Research Part A, 2006, Volume 76A, Number 4, Page 711

Comments (0)

Please log in or register to comment.
Log in