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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

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Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

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Quantitative Determination of Faecal Fatty Acids and Triglycerides by Fourier Transform Infrared Analysis with a Sodium Chloride Transmission Flow Cell

Gerrit Voortman / Jan Gerrits / Maurizio Altavilla / Maurice Henning / Leo van Bergeijk / Jan Hessels

Citation Information: Clinical Chemistry and Laboratory Medicine. Volume 40, Issue 8, Pages 795–798, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2002.137, June 2005

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We studied the applicability of Fourier transform infrared (FTIR) spectroscopy to determine the amount of faecal fatty acids and triglycerides in faeces. For this, we have optimised a simple hexane extraction procedure of stool. After extraction, an aliquot of the hexane layer is directly injected into the measurement cell of the spectrophotometer and absorbance at 1714 cm−1 and 1751 cm−1 is read for quantitation of fatty acids and triglycerides, respectively. Comparison of the FTIR spectrophotometric method with the traditional titrimetric Van de Kamer method for faecal fat determination showed a good correlation. The advantage of our method over the previously described FTIR and nearinfrared spectroscopy (NIR) methods is that it allows a combined measurement of both fatty acids and triglycerides in one run, using a simple and rapid hexane extraction procedure. Clinical validation of this method was performed by reviewing the medical records of 24 patients with steatorrhoea: patients with pancreatic steatorrhoea showed highly increased faecal triglyceride excretion, but a normal faecal fatty acid and faecal weight when compared with patients with nonpancreatic intestinal steatorrhoea.

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