Jump to ContentJump to Main Navigation
Show Summary Details

Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.


IMPACT FACTOR increased in 2015: 3.017
Rank 5 out of 30 in category Medical Laboratory Technology in the 2014 Thomson Reuters Journal Citation Report/Science Edition

SCImago Journal Rank (SJR) 2015: 0.873
Source Normalized Impact per Paper (SNIP) 2015: 0.982
Impact per Publication (IPP) 2015: 2.238

Online
ISSN
1437-4331
See all formats and pricing

 


Select Volume and Issue

Issues

30,00 € / $42.00 / £23.00

Get Access to Full Text

Quantitative Determination of Faecal Fatty Acids and Triglycerides by Fourier Transform Infrared Analysis with a Sodium Chloride Transmission Flow Cell

Gerrit Voortman / Jan Gerrits / Maurizio Altavilla / Maurice Henning / Leo van Bergeijk / Jan Hessels

Citation Information: Clinical Chemistry and Laboratory Medicine. Volume 40, Issue 8, Pages 795–798, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2002.137, June 2005

Publication History

Published Online:
2005-06-01

Abstract

We studied the applicability of Fourier transform infrared (FTIR) spectroscopy to determine the amount of faecal fatty acids and triglycerides in faeces. For this, we have optimised a simple hexane extraction procedure of stool. After extraction, an aliquot of the hexane layer is directly injected into the measurement cell of the spectrophotometer and absorbance at 1714 cm−1 and 1751 cm−1 is read for quantitation of fatty acids and triglycerides, respectively. Comparison of the FTIR spectrophotometric method with the traditional titrimetric Van de Kamer method for faecal fat determination showed a good correlation. The advantage of our method over the previously described FTIR and nearinfrared spectroscopy (NIR) methods is that it allows a combined measurement of both fatty acids and triglycerides in one run, using a simple and rapid hexane extraction procedure. Clinical validation of this method was performed by reviewing the medical records of 24 patients with steatorrhoea: patients with pancreatic steatorrhoea showed highly increased faecal triglyceride excretion, but a normal faecal fatty acid and faecal weight when compared with patients with nonpancreatic intestinal steatorrhoea.

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Fatma Kucuk Baloglu, Sebnem Garip, Sebastian Heise, Gudrun Brockmann, and Feride Severcan
The Analyst, 2015, Volume 140, Number 7, Page 2205
[3]
[4]
Ali Jebali and Bahram Kazemi
Toxicology in Vitro, 2013, Volume 27, Number 6, Page 1847
[5]
S. Sivakumar, J. Sivasubramanian, Chandra Prasad Khatiwada, J. Manivannan, and B. Raja
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2013, Volume 110, Page 241
[6]
Yusuf Baran, Cagatay Ceylan, and Aylin Camgoz
Biomedicine & Pharmacotherapy, 2013, Volume 67, Number 3, Page 221
[7]
S. Sivakumar, J. Sivasubramanian, and B. Raja
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2012, Volume 99, Page 252
[8]
Murugesan Saravanakumar, Jeganathan Manivannan, Jeganathan Sivasubramanian, Thangarasu Silambarasan, Elumalai Balamurugan, and Boobalan Raja
Molecular and Cellular Biochemistry, 2012, Volume 362, Number 1-2, Page 203
[9]
Xiaonan Lu, Molly A.H. Webb, Mariah J. Talbott, Joel P. Van Eenennaam, Serge I. Doroshov, and Barbara A. Rasco
Aquaculture, 2011, Volume 315, Number 3-4, Page 298

Comments (0)

Please log in or register to comment.