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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

12 Issues per year

IMPACT FACTOR 2017: 3.556

CiteScore 2017: 2.34

SCImago Journal Rank (SJR) 2017: 1.114
Source Normalized Impact per Paper (SNIP) 2017: 1.188

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Volume 41, Issue 1


Assessment of Intestinal Permeability: Enzymatic Determination of Urinary Mannitol, Raffinose, Sucrose and Lactose on Hitachi Analyzer

Jan Hessels / Ellen J.M. Snoeyink / Antonius J. Platenkamp / Gerrit Voortman / Jan Steggink / Harry H.M. Eidhof
Published Online: 2005-06-01 | DOI: https://doi.org/10.1515/CCLM.2003.007


The sugar absorption test is the usual test for measurement of intestinal permeability. After intestinal absorption of probe sugars the subsequently excreted sugars are measured in urine. We have developed four enzymatic methods for the measurement of the urinary concentration of the probe sugars mannitol, raffinose, lactose and sucrose. Mannitol, lactose and sucrose are directly measured on Hitachi 917 using mannitol dehydrogenase, β-galactosidase and invertase, respectively, as enzyme reagents. Raffinose measurement needs a three hours preincubation with α-galactosidase, after which the liberated sucrose is measured. The analytical performances such as within- and between-run precision, linearity, lowest detection limit, interference of other sugars and comparison with a gas chromatographic method are described for the four methods. These methods are accurate an can easily be performed in any clinical laboratory.

About the article

Published Online: 2005-06-01

Published in Print: 2003-01-27

Citation Information: Clinical Chemistry and Laboratory Medicine, Volume 41, Issue 1, Pages 33–38, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2003.007.

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