Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

12 Issues per year


IMPACT FACTOR 2017: 3.556

CiteScore 2017: 2.34

SCImago Journal Rank (SJR) 2017: 1.114
Source Normalized Impact per Paper (SNIP) 2017: 1.188

Online
ISSN
1437-4331
See all formats and pricing
More options …
Volume 41, Issue 1

Issues

Assessment of Intestinal Permeability: Enzymatic Determination of Urinary Mannitol, Raffinose, Sucrose and Lactose on Hitachi Analyzer

Jan Hessels / Ellen J.M. Snoeyink / Antonius J. Platenkamp / Gerrit Voortman / Jan Steggink / Harry H.M. Eidhof
Published Online: 2005-06-01 | DOI: https://doi.org/10.1515/CCLM.2003.007

Abstract

The sugar absorption test is the usual test for measurement of intestinal permeability. After intestinal absorption of probe sugars the subsequently excreted sugars are measured in urine. We have developed four enzymatic methods for the measurement of the urinary concentration of the probe sugars mannitol, raffinose, lactose and sucrose. Mannitol, lactose and sucrose are directly measured on Hitachi 917 using mannitol dehydrogenase, β-galactosidase and invertase, respectively, as enzyme reagents. Raffinose measurement needs a three hours preincubation with α-galactosidase, after which the liberated sucrose is measured. The analytical performances such as within- and between-run precision, linearity, lowest detection limit, interference of other sugars and comparison with a gas chromatographic method are described for the four methods. These methods are accurate an can easily be performed in any clinical laboratory.

About the article

Published Online: 2005-06-01

Published in Print: 2003-01-27


Citation Information: Clinical Chemistry and Laboratory Medicine, Volume 41, Issue 1, Pages 33–38, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2003.007.

Export Citation

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Jacopo Gervasoni, Arcangelo Schiattarella, Valentina Giorgio, Aniello Primiano, Consuelo Russo, Valentina Tesori, Franco Scaldaferri, Andrea Urbani, Cecilia Zuppi, and Silvia Persichilli
Disease Markers, 2016, Volume 2016, Page 1
[3]
Simon Tännler, Nicola Zamboni, Csilla Kiraly, Stéphane Aymerich, and Uwe Sauer
Metabolic Engineering, 2008, Volume 10, Number 5, Page 216
[4]
Jan Hessels, Harry H. M. Eidhof, Jan Steggink, Wilfried W. H. Roeloffzen, Kalung Wu, Guy Tan, Jan van de Stadt, and Leo van Bergeijk
Clinical Chemistry and Laboratory Medicine, 2003, Volume 41, Number 8
[5]
Milan Dastych, Milan Dastych, Hana Novotná, and J. Číhalová
Digestive Diseases and Sciences, 2008, Volume 53, Number 10, Page 2789

Comments (0)

Please log in or register to comment.
Log in