Jump to ContentJump to Main Navigation
Show Summary Details

Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

12 Issues per year

IMPACT FACTOR increased in 2015: 3.017
Rank 5 out of 30 in category Medical Laboratory Technology in the 2014 Thomson Reuters Journal Citation Report/Science Edition

SCImago Journal Rank (SJR) 2015: 0.873
Source Normalized Impact per Paper (SNIP) 2015: 0.982
Impact per Publication (IPP) 2015: 2.238

See all formats and pricing
Volume 41, Issue 12 (Dec 2003)


Quantitative Analysis of Bile Acids in Human Plasma by Liquid Chromatography-Electrospray Tandem Mass Spectrometry: A Simple and Rapid One-Step Method

Debora Tagliacozzi / Alessia F. Mozzi / Bruno Casetta / Pierfrancesco Bertucci / Sergio Bernardini / Carmine Di Ilio / Andrea Urbani / Giorgio Federici
Published Online: 2005-06-01 | DOI: https://doi.org/10.1515/CCLM.2003.247


Bile acids play a pivotal role in the metabolism of cholesterol and lipids. Their blood concentrations are important prognostic and diagnostic indicators of hepatobiliary and intestinal dysfunction. This class of molecules comprises a heterogeneous group of compounds with a common cholesterol scaffold. Recently, the introduction of liquid chromatography coupled to tandem mass spectrometry methods has revealed an innovative path in the quantisation of specific bile acids in biological specimens. A robust and sensitive method has been developed based on high performance liquid chromatography separation coupled to an electrospray triple-quadrupole mass spectrometer. Human plasma samples were analysed on a C18 reverse-phase column. The elution profiles were monitored in multiple reaction-monitoring mode, quantifying and identifying each analyte by its own unique precursor to product patterns. A linear correlation over a broad range of bile acid concentrations (0.1–100 μM) was observed. The average recovery period for all of the analysed bile acids was 98±3%. Intra-day and inter-day precision averages were 2% and 5.4%, respectively. The determination was achieved within a single chromatographic run for all unconjugated, glycine-and taurine-conjugated isomeric forms of bile acids. As a proof of principle this method has been validated on a small subset of cholestatic patients (n = 7) and compared to appropriate clinical controls (n = 10).

Based upon our encouraging experimental results, the described HPLC separation coupled to tandem mass spectrometry method for the analysis of bile acids in biological samples is deemed a robust and accurate procedure. Consequently, we propose this technique as a suitable candidate method for the identification and quantitation of bile acids in routine analysis.

About the article

Published Online: 2005-06-01

Published in Print: 2003-12-04

Citation Information: Clinical Chemistry and Laboratory Medicine, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2003.247. Export Citation

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

H. Graffner, P.-G. Gillberg, L. Rikner, and H.-U. Marschall
Alimentary Pharmacology & Therapeutics, 2015, Page n/a
Jörg Jahnel, Evelyn Zöhrer, Hubert Scharnagl, Wolfgang Erwa, Günter Fauler, and Tatjana Stojakovic
Clinical Chemistry and Laboratory Medicine (CCLM), 2015, Volume 53, Number 11
Ke Li, Tyler J. Buchinger, Ugo Bussy, Skye D. Fissette, Nicholas S. Johnson, and Weiming Li
Journal of Chromatography B, 2015
R Dutia, M Embrey, S O'Brien, R A Haeusler, K K Agénor, P Homel, J McGinty, R P Vincent, J Alaghband-Zadeh, B Staels, C W le Roux, J Yu, and B Laferrère
International Journal of Obesity, 2015, Volume 39, Number 5, Page 806
Patricia Walker and Daniel Lee
Journal of Cosmetic Dermatology, 2015, Volume 14, Number 1, Page 33
Gideon M. Hirschfield, Andrew Mason, Velimir Luketic, Keith Lindor, Stuart C. Gordon, Marlyn Mayo, Kris V. Kowdley, Catherine Vincent, Henry C. Bodhenheimer, Albert Parés, Michael Trauner, Hanns-Ulrich Marschall, Luciano Adorini, Cathi Sciacca, Tessa Beecher-Jones, Erin Castelloe, Olaf Böhm, and David Shapiro
Gastroenterology, 2015, Volume 148, Number 4, Page 751
M. Camilleri, A. Acosta, I. Busciglio, A. Boldingh, R. B. Dyer, A. R. Zinsmeister, A. Lueke, A. Gray, and L. J. Donato
Alimentary Pharmacology & Therapeutics, 2015, Volume 41, Number 5, Page 438
Huiyong Wang, Chu-Yin Yeh, Ke Li, Yu-Wen Chung-Davidson, and Weiming Li
Journal of Chromatography B, 2015, Volume 980, Page 72
Jun Han, Yang Liu, Renxue Wang, Juncong Yang, Victor Ling, and Christoph H. Borchers
Analytical Chemistry, 2015, Volume 87, Number 2, Page 1127
M. Camilleri, A. Shin, I. Busciglio, P. Carlson, A. Acosta, A. E. Bharucha, D. Burton, J. Lamsam, A. Lueke, L. J. Donato, and A. R. Zinsmeister
Neurogastroenterology & Motility, 2014, Volume 26, Number 12, Page 1677
Michael Camilleri, Irene Busciglio, Andres Acosta, Andrea Shin, Paula Carlson, Duane Burton, Michael Ryks, Deborah Rhoten, Jesse Lamsam, Alan Lueke, Leslie J Donato, and Alan R Zinsmeister
The American Journal of Gastroenterology, 2014, Volume 109, Number 10, Page 1621
F. J. Reen, D. F. Woods, M. J. Mooij, M. N. Chróinín, D. Mullane, L. Zhou, J. Quille, D. Fitzpatrick, J. D. Glennon, G. P. McGlacken, C. Adams, and F. O’Gara
European Journal of Clinical Microbiology & Infectious Diseases, 2014, Volume 33, Number 10, Page 1763
Sai Praneeth R. Bathena, Sandeep Mukherjee, Marco Olivera, and Yazen Alnouti
Journal of Chromatography B, 2013, Volume 942-943, Page 53
Malin Werling, Royce P Vincent, Gemma F Cross, Hanns-Ulrich Marschall, Lars Fändriks, Hans Lönroth, David R Taylor, Jamshid Alaghband-Zadeh, Torsten Olbers, and Carel W Le Roux
Scandinavian Journal of Gastroenterology, 2013, Volume 48, Number 11, Page 1257
Robert E. Steinert, Ralph Peterli, Sylvia Keller, Anne C. Meyer-Gerspach, Jürgen Drewe, Thomas Peters, and Christoph Beglinger
Obesity, 2013, Volume 21, Number 12, Page E660
Sunder Mudaliar, Robert R. Henry, Arun J. Sanyal, Linda Morrow, Hanns–Ulrich Marschall, Mark Kipnes, Luciano Adorini, Cathi I. Sciacca, Paul Clopton, Erin Castelloe, Paul Dillon, Mark Pruzanski, and David Shapiro
Gastroenterology, 2013, Volume 145, Number 3, Page 574
Troy Voelker, Hongxia Wang, Mitzi Irish, Juan Wang, Stephanie Harrison, Scott Reuschel, Patrick Bennett, and Min Meng
Bioanalysis, 2013, Volume 5, Number 10, Page 1229
Lydie Humbert, Marie Anne Maubert, Claude Wolf, Henri Duboc, Myriam Mahé, Dominique Farabos, Philippe Seksik, Jean Maurice Mallet, Germain Trugnan, Joëlle Masliah, and Dominique Rainteau
Journal of Chromatography B, 2012, Volume 899, Page 135
Manuel Sergi, Camilla Montesano, Sabino Napoletano, Daniel Pizzoni, Cesare Manetti, Franco Colistro, Roberta Curini, and Dario Compagnone
Chromatographia, 2012, Volume 75, Number 9-10, Page 479
Jocelyn Trottier, Andrzej Białek, Patrick Caron, Robert J. Straka, Jenny Heathcote, Piotr Milkiewicz, and Olivier Barbier
Digestive and Liver Disease, 2012, Volume 44, Number 4, Page 303
Tracy L. Marion, Cassandra H. Perry, Robert L. St. Claire, and Kim L.R. Brouwer
Toxicology and Applied Pharmacology, 2012, Volume 261, Number 1, Page 1
Junhai Ou, James P. DeLany, Ming Zhang, Sumit Sharma, and Stephen J. D. O’Keefe
Nutrition and Cancer, 2012, Volume 64, Number 1, Page 34
Ivana Bobeldijk, Maarten Hekman, Jitske de Vries-van der Weij, Leon Coulier, Raymond Ramaker, Robert Kleemann, Teake Kooistra, Carina Rubingh, Andreas Freidig, and Elwin Verheij
Journal of Chromatography B, 2008, Volume 871, Number 2, Page 306
Gary N.W. Leung, Evonne W. Chung, Emmie N.M. Ho, W.H. Kwok, David K.K. Leung, Francis P.W. Tang, Terence S.M. Wan, and Nola H. Yu
Journal of Chromatography B, 2005, Volume 825, Number 1, Page 47
MaryPeace McRae, Naser L Rezk, Arlene S Bridges, Amanda H Corbett, Hsiao-Chuan Tien, Kim L. R Brouwer, and Angela D. M Kashuba
Pharmacotherapy, 2010, Volume 30, Number 1, Page 17
T. L. Marion, C. H. Perry, R. L. St. Claire, W. Yue, and K. L. R. Brouwer
Toxicological Sciences, 2011, Volume 120, Number 2, Page 371
R. E. Roberts, C. Glicksman, J. Alaghband-Zadeh, R. A. Sherwood, N. Akuji, and C. W. le Roux
Clinical Endocrinology, 2011, Volume 74, Number 1, Page 67
Yimin Wu, Xiaochun Wang, Qingzheng Wu, Xiaoping Wu, Xucong Lin, and Zenghong Xie
Analytical Methods, 2010, Volume 2, Number 12, Page 1927
B. Natalini, R. Sardella, E. Camaioni, S. Natalini, and R. Pellicciari
Chromatographia, 2006, Volume 64, Number 5-6, Page 343

Comments (0)

Please log in or register to comment.
Log in