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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Greaves, Ronda / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter


IMPACT FACTOR 2018: 3.638

CiteScore 2018: 2.44

SCImago Journal Rank (SJR) 2018: 1.191
Source Normalized Impact per Paper (SNIP) 2018: 1.205

Online
ISSN
1437-4331
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Volume 41, Issue 4

Issues

Electrochemical DNA Sensor for Detection of Single Nucleotide Polymorphisms

Leonel P. J. Marques / Isa Cavaco / José P. Pinheiro / Vera Ribeiro / Guilherme N.M. Ferreira
Published Online: 2005-06-01 | DOI: https://doi.org/10.1515/CCLM.2003.071

Abstract

In recent years there has been an increased interest in using biosensors for the recognition and monitoring of molecule interactions. DNA sensors and gene chips are particularly relevant for directly applying the information gathered from the genome projects. In this work electrochemical techniques are used to develop methodologies to detect DNA polymorphisms in human genes using cytochrome P450 3A4 (CYP3A4) as a model gene. CYP3A4*1B oligonucleotides were immobilized on the surface of a gold electrode and hybridized with fully complementary oligonucleotide sequences as well as with mismatched sequences corresponding to the CYP3A4*1A reference sequence. The methodology developed is based on double-stranded DNA's ability to transport charge along nucleotide stacking. The perturbation of the double helix pi-stack introduced by a mismatched nucleotide reduces electron flow and can be detected by measuring the attenuation of the charge transfer. The methodology developed could identify CYP3A4*1A homozygotes by the 5 μC charge attenuation observed when compared with DNA samples containing at least one CYP3A4*1B allele.

About the article

Published Online: 2005-06-01

Published in Print: 2003-04-25


Citation Information: Clinical Chemistry and Laboratory Medicine, Volume 41, Issue 4, Pages 475–481, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2003.071.

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