Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

12 Issues per year

IMPACT FACTOR 2016: 3.432

CiteScore 2016: 2.21

SCImago Journal Rank (SJR) 2016: 1.000
Source Normalized Impact per Paper (SNIP) 2016: 1.112

See all formats and pricing
More options …
Volume 41, Issue 5 (May 2003)


Automated Serum Protein Electrophoresis by Capillarys®

Xavier Bossuyt / Bénédicte Lissoir / Godelieve Mariën / Diane Maisin / Jozef Vunckx / Norbert Blanckaert / Pierre Wallemacq
Published Online: 2005-06-01 | DOI: https://doi.org/10.1515/CCLM.2003.107


Capillary zone electrophoresis (CZE) of serum proteins is increasingly gaining impact in clinical laboratories. In this report, we evaluate automated capillary zone electrophoresis by Capillarys® (Sebia, France). Withinrun and between-run imprecision for the five electrophoretic fractions was <2% and <6%, respectively. Data obtained with Capillarys correlated with results obtained with agarose gel electrophoresis and Paragon CZE® 2000 (Beckman Coulter, USA). Analysis of serum obtained from patients with inflammation, nephrotic syndrome, bisalbuminemia, and α1-antitrypsin deficiency revealed that Capillarys was able to detect these abnormalities. Two hundred thirty eight samples were analyzed by agarose gel electrophoresis, Capillarys, capillary electrophoresis using Paragon CZE 2000 system, and immunofixation. Sample selection was based on the presence of a disturbed morphology (e.g., spike) of the protein profile or hypogammaglobulinemia on agarose gel electrophoresis and/or Capillarys. Immunofixation revealed the presence of a monoclonal protein, oligoclonal bands, polyclonal pattern, and a normal profile in, respectively, 89, 66, 19, and 64 samples. With Capillarys, Paragon, and agarose gel electrophoresis, a spike and/or disturbed morphology of the profile was found in 222, 182, and 180 samples, respectively. In these samples, immunofixation was negative in 73 (33%), 46 (25%), and 39 (22%) samples, respectively. These data indicate that Capillarys has a lower specificity than agarose gel electrophoresis and Paragon 2000. Of the 89 samples with a monoclonal protein, Capillarys, Paragon, and agarose gel electrophoresis failed to detect, respectively, three, three, and one monoclonal protein(s). Interferences by radio-opaque agents, complement degradation products, fibrinogen, and triglycerides are described. In conclusion, automated capillary zone electrophoresis with Capillarys provides for reproducible, rapid, and reliable serum electrophoresis.

About the article

Published Online: 2005-06-01

Published in Print: 2003-05-15

Citation Information: Clinical Chemistry and Laboratory Medicine, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2003.107.

Export Citation

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

Yannick Roman, Marie-Claude Bomsel-Demontoy, Julie Levrier, Daniel Chaste-Duvernoy, and Michel Saint Jalme
Journal of Avian Medicine and Surgery, 2013, Volume 27, Number 2, Page 99
Laurence Guis, Anelise Chaumier, Virginie Le Gall, and Sabine Havrez
Revue Francophone des Laboratoires, 2013, Volume 2013, Number 449, Page 47
C. Brousse
EMC - Tratado de Medicina, 2008, Volume 12, Number 3, Page 1
Florence Masaisa, Jean Bosco Gahutu, Joshua Mukiibi, Joris Delanghe, and Jan Philippé
Acta Haematologica, 2012, Volume 128, Number 2, Page 100
Evy De Witte, Marijn M. Speeckaert, Lot Van De Moortel, Elke Lecocq, and Joris R. Delanghe
ELECTROPHORESIS, 2012, Volume 33, Number 3, Page 440
Fabienne Parente, Nicholas Ah Mew, Jaak Jaeken, and Brian M. Gilfix
Clinica Chimica Acta, 2010, Volume 411, Number 1-2, Page 64
Xavier Bossuyt
Clinical Chemistry and Laboratory Medicine, 2003, Volume 41, Number 6
Alessandro Terreni, Anna Caldini, Agostino Ognibene, Benedetta Salvadori, and Gianni Messeri
Laboratory Medicine, 2006, Volume 37, Number 4, Page 233
Lixin Zhang, Stéphanie Albarède, Gilles Dumont, Christel Van Campenhout, Jean-Claude Libeer, and Adelin Albert
Accreditation and Quality Assurance, 2010, Volume 15, Number 6, Page 351
Dieter Vanderschaeghe, Evi Debruyne, Hans Van Vlierberghe, Nico Callewaert, and Joris Delanghe
ELECTROPHORESIS, 2009, Volume 30, Number 15, Page 2617
Lixin Zhang, Christel Van Campenhout, Nicole Devleeschouwer, Jean-Claude Libeer, and Adelin Albert
Accreditation and Quality Assurance, 2008, Volume 13, Number 3, Page 149
Rukhsana Jabeen, Deborah Payne, John Wiktorowicz, Amin Mohammad, and John Petersen
ELECTROPHORESIS, 2006, Volume 27, Number 12, Page 2413
Vladislav Dolník
ELECTROPHORESIS, 2006, Volume 27, Number 1, Page 126

Comments (0)

Please log in or register to comment.
Log in