Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

12 Issues per year

IMPACT FACTOR 2017: 3.556

CiteScore 2017: 2.34

SCImago Journal Rank (SJR) 2017: 1.114
Source Normalized Impact per Paper (SNIP) 2017: 1.188

See all formats and pricing
More options …
Volume 41, Issue 8


Effects of Vitamin B12 and Folate Deficiencies on DNA Methylation and Carcinogenesis in Rat Liver

Laurent Brunaud / Jean-Marc Alberto / Ahmet Ayav / Philippe Gérard / Farès Namour / Laurent Antunes / Marc Braun / Jean-Pierre Bronowicki / Laurent Bresler / Jean-Louis Guéant
Published Online: 2005-06-01 | DOI: https://doi.org/10.1515/CCLM.2003.155


Deficiencies of the major dietary sources of methyl groups, methionine and choline, lead to the formation of liver cancer in rodents. The most widely investigated hypothesis has been that dietary methyl insufficiency results in abnormal DNA methylation. Vitamin B12 and folate also play important roles in DNA methylation since these two coenzymes are required for the synthesis of methionine and S-adenosyl methionine, the common methyl donor required for the maintenance of methylation patterns in DNA. The aim of this study was to review the effects of methyl-deficient diets on DNA methylation and liver carcinogenesis in rats, and to evaluate the role of vitamin B12 status in defining carcinogenicity of a methyl-deficient diet. Several studies have shown that a methyl-deficient diet influences global DNA methylation. Evidence from in vivo studies has not clearly established a link between vitamin B12 and DNA methylation. We reported that vitamin B12 and low methionine synthase activity were the two determinants of DNA hypomethylation. Choline- or choline/methionine-deficient diets have been shown to cause hepatocellular carcinoma in 20–50% of animals after 12–24 months. In contrast, the effect of vitamin B12 withdrawal, in addition to choline, methionine and folate, induced hepatocellular carcinoma in less than 5% of

About the article

Published Online: 2005-06-01

Published in Print: 2003-08-07

Citation Information: Clinical Chemistry and Laboratory Medicine, Volume 41, Issue 8, Pages 1012–1019, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2003.155.

Export Citation

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

Zhao-Yan Liu, Xu-Ying Tan, Qi-Jiong Li, Gong-Cheng Liao, Ai-Ping Fang, Dao-Ming Zhang, Pei-Yan Chen, Xiao-Yan Wang, Yun Luo, Jing-An Long, Rong-Huan Zhong, and Hui-Lian Zhu
Nutrition & Metabolism, 2018, Volume 15, Number 1
Manish K. Yadav, Nandini M. Manoli, Selvaraj Vimalraj, and SubbaRao V. Madhunapantula
International Journal of Biological Macromolecules, 2017
Istvan Seffer, Zoltan Nemeth, Gyula Hoffmann, Robert Matics, A. Gergely Seffer, and Akos Koller
Genetics & Epigenetics, 2013, Volume 5, Page GEG.S11752
Cindy D. Davis and Eric O. Uthus
Experimental Biology and Medicine, 2004, Volume 229, Number 10, Page 988
Manish K. Yadav, Nandini M. Manoli, SubbaRao V. Madhunapantula, and Klaus Roemer
PLOS ONE, 2016, Volume 11, Number 10, Page e0164559
Eoin P. Quinlivan, Krista S. Crider, Jiang-Hui Zhu, David R. Maneval, Ling Hao, Zhu Li, Sonja A. Rasmussen, R. J. Berry, Lynn B. Bailey, and Guoliang Xu
PLoS ONE, 2013, Volume 8, Number 6, Page e65241
Jennifer A. Rusiecki, Laura E. Beane Freeman, Matthew R. Bonner, Melannie Alexander, Ligong Chen, Gabriella Andreotti, Kathryn H. Barry, Lee E. Moore, Hyang-Min Byun, Freya Kamel, Michael Alavanja, Jane A. Hoppin, and Andrea Baccarelli
Environmental and Molecular Mutagenesis, 2017, Volume 58, Number 1, Page 19
Dan Zhou, Ryan A. Hlady, Marissa J. Schafer, Thomas A. White, Chen Liu, Jeong-Hyeon Choi, Jordan D. Miller, Lewis R. Roberts, Nathan K. LeBrasseur, and Keith D. Robertson
Epigenetics, 2017, Volume 12, Number 1, Page 55
Fernando Salvador Moreno, Renato Heidor, and Igor P. Pogribny
Nutrition and Cancer, 2016, Volume 68, Number 5, Page 719
Minjun Chen, Ayako Suzuki, Jürgen Borlak, Raúl J. Andrade, and M Isabel Lucena
Journal of Hepatology, 2015, Volume 63, Number 2, Page 503
Shen-Chih Chang, Binh Y. Goldstein, Lina Mu, Lin Cai, Nai-Chieh Y. You, Na He, Bao-Guo Ding, Jin-Kou Zhao, Shun-Zhang Yu, David Heber, Zuo-Feng Zhang, and Qing-Yi Lu
Nutrition and Cancer, 2015, Volume 67, Number 2, Page 212
Baitang Ning, Zhenqiang Su, Nan Mei, Huixiao Hong, Helen Deng, Leming Shi, James C. Fuscoe, and William H. Tolleson
Journal of Environmental Science and Health, Part C, 2014, Volume 32, Number 2, Page 121
Inna I. Kruman and Anna-Kate Fowler
Journal of Neurochemistry, 2014, Volume 129, Number 5, Page 770
Patadon Sukapan, Paramate Promnarate, Yingyos Avihingsanon, Apiwat Mutirangura, and Nattiya Hirankarn
Journal of Human Genetics, 2014, Volume 59, Number 4, Page 178
P. Cordero, A. M. Gomez-Uriz, J. Campion, F. I. Milagro, and J. A. Martinez
Genes & Nutrition, 2013, Volume 8, Number 1, Page 105
Erminio Giavini and Elena Menegola
Reproductive Toxicology, 2012, Volume 34, Number 2, Page 180
Dionne N. Shepherd, Darren P. Martin, Arvind Varsani, Jennifer A. Thomson, Edward P. Rybicki, and Horst H. Klump
Archives of Biochemistry and Biophysics, 2006, Volume 453, Number 1, Page 108
Michael D. Voigt
Clinics in Liver Disease, 2005, Volume 9, Number 1, Page 151
Igor P. Pogribny, S. Jill James, and Frederick A. Beland
Molecular Nutrition & Food Research, 2012, Volume 56, Number 1, Page 116
Tomas Honzik, Miriam Adamovicova, Vratislav Smolka, Martin Magner, Eva Hruba, and Jiri Zeman
European Journal of Paediatric Neurology, 2010, Volume 14, Number 6, Page 488
Flore Depeint, W. Robert Bruce, Nandita Shangari, Rhea Mehta, and Peter J. O’Brien
Chemico-Biological Interactions, 2006, Volume 163, Number 1-2, Page 113
Nakarin Kitkumthorn and Apiwat Mutirangura
Clinical Epigenetics, 2011, Volume 2, Number 2, Page 315
Moshe Szyf
Biochimica et Biophysica Acta (BBA) - General Subjects, 2009, Volume 1790, Number 9, Page 878
Igor P. Pogribny, Sharon A. Ross, Carolyn Wise, Marta Pogribna, Elisabeth A. Jones, Volodymyr P. Tryndyak, S. Jill James, Yvonne P. Dragan, and Lionel A. Poirier
Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 2006, Volume 593, Number 1-2, Page 80
Jennifer A. Rusiecki, Andrea Baccarelli, Valentina Bollati, Letizia Tarantini, Lee E. Moore, and Eva C. Bonefeld-Jorgensen
Environmental Health Perspectives, 2008, Volume 116, Number 11, Page 1547
Joseph R. Dobosy, Vivian X. Fu, Joshua A. Desotelle, Rajini Srinivasan, Michelle L. Kenowski, Nima Almassi, Richard Weindruch, John Svaren, and David F. Jarrard
The Prostate, 2008, Volume 68, Number 11, Page 1187
Giuliana Muzio, Marina Maggiora, Manuela Oraldi, Antonella Trombetta, and Rosa Angela Canuto
International Journal of Cancer, 2007, Volume 121, Number 11, Page 2395
Lynnette R. Ferguson, Nishi Karunasinghe, and Martin Philpott
Environmental and Molecular Mutagenesis, 2004, Volume 44, Number 1, Page 36

Comments (0)

Please log in or register to comment.
Log in