Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Greaves, Ronda / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter

IMPACT FACTOR 2018: 3.638

CiteScore 2018: 2.44

SCImago Journal Rank (SJR) 2018: 1.191
Source Normalized Impact per Paper (SNIP) 2018: 1.205

See all formats and pricing
More options …
Volume 42, Issue 1


Evaluation of a new efficient procedure for single-nucleotide polymorphism genotyping: tetra-primer amplification refractory mutation system-polymerase chain reaction

Naoko Okayama / Kozue Fujimura / Junji Nakamura / Yutaka Suehiro / Yuichiro Hamanaka / Yuji Hinoda
Published Online: 2005-06-01 | DOI: https://doi.org/10.1515/CCLM.2004.004


Tetra-primer amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) is a new efficient method for single-nucleotide polymorphism (SNP) genotyping. To determine the optimal conditions for ARMS-PCR we attempted to genotype ten SNPs. DNA was extracted from the peripheral blood of 168 unrelated healthy Japanese volunteers. Two problems inhibited uniform efficiency of the amplification of three bands. The first problem was the lower amplification efficiency of the shorter and allele-specific products compared with the largest product. This phenomenon was overcome by increasing the relative concentration of the inner primers. The second problem was non-specific amplification of the shorter products. To reduce the amplification of these nonspecific bands, adjusting any one of the following PCR conditions was effective: i) reducing the ratio of the inner primer concentration relative to that of the outer primers; ii) increasing the annealing temperature for the initial 5–10 cycles; iii) hot start PCR. With these procedures all ten of the SNPs were successfully genotyped. Our present data may be useful in the further application of tetra-primer ARMS-PCR to SNP genotyping.

About the article

Published Online: 2005-06-01

Published in Print: 2004-02-16

Citation Information: Clinical Chemistry and Laboratory Medicine (CCLM), Volume 42, Issue 1, Pages 13–16, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2004.004.

Export Citation

Copyright © 2004 by Walter de Gruyter GmbH & Co. KG.Get Permission

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

Hai-Bo Wang, Li-Hong Ma, Ting Zhang, Kai-Chen Huang, Yuan-Di Zhao, and Tian-Cai Liu
Analytica Chimica Acta, 2019
Dan Zeng, Xiao Li, Xiao-qing Wang, and Gang Xiong
Conservation Genetics Resources, 2018
Kazuhiko Sakamoto, Shigeru Takeda, Shinsuke Kanekiyo, Mitsuo Nishiyama, Masahiro Kitahara, Tomio Ueno, Shigeru Yamamoto, Shigefumi Yoshino, Shoichi Hazama, Naoko Okayama, and Hiroaki Nagano
Molecular and Clinical Oncology, 2017, Volume 6, Number 1, Page 125
Sonika Ahlawat, Rekha Sharma, A. Maitra, Manoranjan Roy, and M.S. Tantia
Meta Gene, 2014, Volume 2, Page 439
Kazuhiko Sakamoto, Masaaki Oka, Shigehumi Yoshino, Shoichi Hazama, Shigeru Takeda, Kiyoshi Yoshimura, Naoko Okayama, and Yuji Hinoda
Journal of Gastrointestinal Surgery, 2014, Volume 18, Number 7, Page 1247
Shuai Zhang, Patrick Kwan, and Larry Baum
Neuroscience Letters, 2013, Volume 556, Page 146
R. Marquis-Nicholson, E. Glamuzina, D. Prosser, C. Wilson, and D.R. Love
Genetics and Molecular Research, 2010, Volume 9, Number 3, Page 1483
Quanyuan Wan, Jianguo Su, Xiaohui Chen, Chunrong Yang, Lijun Chen, Nana Yan, and Yixuan Zhang
Developmental & Comparative Immunology, 2013, Volume 39, Number 4, Page 333
Wei Li, Yanli Zeng, Junjie Wang, Bin Zhou, Jian Zhang, Hao Zhang, Jingtao Li, Yingsong Wu, Rifat Hamoudi, and Yuanping Zhou
Antiviral Research, 2012, Volume 94, Number 1, Page 54
José Ignacio Ruiz-Sanz, Igor Aurrekoetxea, Ainhoa Ruiz del Agua, and María Begoña Ruiz-Larrea
Molecular and Cellular Probes, 2007, Volume 21, Number 3, Page 202
Ozdal Etlik, Vedat Koksal, S. Tugba Arican-Baris, and Ibrahim Baris
Molecular and Cellular Probes, 2011, Volume 25, Number 4, Page 177
N. Okayama, Y. Suehiro, Y. Hamanaka, J. Nakamura, and Y. Hinoda
The Journals of Gerontology Series A: Biological Sciences and Medical Sciences, 2007, Volume 62, Number 5, Page 507
Maja Živković, Aleksandar Raković, and Aleksandra Stanković
Clinical Biochemistry, 2006, Volume 39, Number 6, Page 630
N. Okayama, Y. Hamanaka, Y. Suehiro, Y. Hasui, J. Nakamura, and Y. Hinoda
The Journals of Gerontology Series A: Biological Sciences and Medical Sciences, 2005, Volume 60, Number 12, Page 1525
Paolo Donini, Manuel Rubio, Carole Caranta, and Alain Palloix
Genome, 2008, Volume 51, Number 9, Page 767
Hai Thi Hong Truong, Ki-Taek Kim, Su Kim, Myoung-Cheol Cho, Hyun-Ran Kim, and Jong-Gyu Woo
Horticulture, Environment, and Biotechnology, 2011, Volume 52, Number 1, Page 65
Tomoko Hashimoto, Kenichiro Uchida, Naoko Okayama, Yuji Imate, Yutaka Suehiro, Yuichiro Hamanaka, Yoshiya Ueyama, Hiroshi Yamashita, and Yuji Hinoda
Molecular Carcinogenesis, 2006, Volume 45, Number 5, Page 344
Yoshinori Hasui, Yuichiro Hamanaka, Naoko Okayama, Yutaka Suehiro, Fumihiko Shinozaki, Yoshiya Ueyama, and Yuji Hinoda
Journal of Clinical Laboratory Analysis, 2006, Volume 20, Number 2, Page 47
Yuji Hinoda
SEIBUTSU BUTSURI KAGAKU, 2004, Volume 48, Number 4, Page 147

Comments (0)

Please log in or register to comment.
Log in