Jump to ContentJump to Main Navigation
Show Summary Details

Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

IMPACT FACTOR increased in 2015: 3.017
Rank 5 out of 30 in category Medical Laboratory Technology in the 2014 Thomson Reuters Journal Citation Report/Science Edition

SCImago Journal Rank (SJR) 2015: 0.873
Source Normalized Impact per Paper (SNIP) 2015: 0.982
Impact per Publication (IPP) 2015: 2.238

See all formats and pricing


Select Volume and Issue


30,00 € / $42.00 / £23.00

Get Access to Full Text

Matrix effects in clinical immunoassays and the effect of preheating and cooling analytical samples

Hiroshi Yoshida / Yuji Imafuku / Toshihiko Nagai

Citation Information: Clinical Chemistry and Laboratory Medicine. Volume 42, Issue 1, Pages 51–56, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2004.010, June 2005

Publication History

Published Online:


Immunological reactions are influenced by various factors including antigens, antibodies and other variables. We focused on two items: i) matrix effects, especially of detergents and ii) temperature effects: preheating sera, especially effects on rheumatoid factor (RF) measurement and false-positive reactions in ELISAs, and cold storage of sera, especially effects on complement. Among various additives, detergents affected the agglutination reaction for fecal hemoglobin and hepatitis B surface (HBs) antigen. Some of the detergents examined abolished these antigenicities, however, polyethylenglycols enhanced the reactions. Heat-inactivation of sera at 56°C for 30 min was employed in serological testing. However, in RF measurement, 10 min of preheating was sufficient to abolish C1q (subcomponent of C1), which could participate in the agglutination reaction. In ELISA for antibodies, false-positive reactions were caused by preheating sera. By the analyses of assays for antibodies to hepatitis C virus (HCV) and cardiolipin, it was found that they were induced by immunoglobulin G (IgG) modified by preheating. Cold storage induced activation of complement (cold activation) in anti-HCV antibody positive sera. CH50 titers in the sera were lowered by one cycle of freezing at −20°C and thawing, and the decrease was affected by the containers.

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

Roland F Staack, Gregor Jordan, Uwe Dahl, and Julia Heinrich
Bioanalysis, 2014, Volume 6, Number 4, Page 485
Yong Huang, Robert Shi, Winnie Gee, and Richard Bonderud
Bioanalysis, 2012, Volume 4, Number 3, Page 271
Tine Dalby, Maria Seier-Petersen, Max Per Kristiansen, Zitta Barrella Harboe, and Karen Angeliki Krogfelt
Diagnostic Microbiology and Infectious Disease, 2009, Volume 63, Number 4, Page 354
Resul Karakus, Bugra A. Buyrukcu, and Cemalettin Aybay
Journal of Immunoassay and Immunochemistry, 2005, Volume 26, Number 2, Page 109
Kevin Ashley, Raymond E. Biagini, Jerry P. Smith, Deborah L. Sammons, Barbara A. MacKenzie, Cynthia A.F. Striley, Shirley K. Robertson, and John E. Snawder
Journal of Occupational and Environmental Hygiene, 2008, Volume 5, Number 3, Page D37
L. Strandberg, O. Winqvist, S.-E. Sonesson, S. Mohseni, S. Salomonsson, K. Bremme, J. P. Buyon, H. Julkunen, and M. Wahren-Herlenius
Clinical & Experimental Immunology, 2008, Volume 154, Number 1, Page 30

Comments (0)

Please log in or register to comment.