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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

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1437-4331
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Volume 42, Issue 10 (Oct 2004)

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Screening of antinuclear antibodies: comparison between enzyme immunoassay based on nuclear homogenates, purified or recombinant antigens and immunofluorescence assay

Sergio Bernardini
  • Department of Internal Medicine and Department of Laboratory Medicine-PTV, University of Rome “Tor Vergata”, Rome, Italy
/ Maria Infantino
  • Department of Internal Medicine and Department of Laboratory Medicine-PTV, University of Rome “Tor Vergata”, Rome, Italy
/ Lorenza Bellincampi
  • Department of Internal Medicine and Department of Laboratory Medicine-PTV, University of Rome “Tor Vergata”, Rome, Italy
/ Marzia Nuccetelli
  • Department of Internal Medicine and Department of Laboratory Medicine-PTV, University of Rome “Tor Vergata”, Rome, Italy
/ Antonella Afeltra
  • Campus-Biomedico, Rome, Italy
/ Roberta Iori
  • Department of Internal Medicine and Department of Laboratory Medicine-PTV, University of Rome “Tor Vergata”, Rome, Italy
/ Antonino Biroccio
  • Bambino Gesù-IRCCS Hospital, Rome, Italy
/ Andrea Urbani
  • Department of Biomedical Sciences, University “G. D’Annunzio” and Ce.S.I., University Foundation “G. D’Annunzio”, Chieti, Italy
/ Giorgio Federici
  • Department of Internal Medicine and Department of Laboratory Medicine-PTV, University of Rome and Bambino Gesù-IRCCS Hospital, Rome, Italy
Published Online: 2011-09-21 | DOI: https://doi.org/10.1515/CCLM.2004.235

Abstract

Current clinical practice considers antinuclear antibody (ANA) testing as a screening test; this has a major impact on laboratory work with a growing volume of analyses that need to be performed rapidly, to maintain good specificity and sensitivity. Ongoing discussions have been raised in order to identify the best technology to use in ANA screening, taking into account both clinical and economical implications. The aim of our study was to compare three different enzyme immunoassays (EIA) with immunofluorescence (IF) assay in order to identify which test is better for use as a screening test. The study was performed on 473 sera and the three different EIA tests were based on nuclear homogenates from HeLa cells, purified antigens from HEp-2 cells and recombinant antigens, respectively. The concordance between EIA-ANA and IF-ANA techniques, determined by the κ statistic, was acceptable, but not complete, and discrepancies between both EIA-positive/IF-negative samples and IF-positive/EIA-negative were found. Both methods show interesting diagnostic abilities, however, the IF-ANA assay seems to be the first choice test in a well-standardized immunofluorescence laboratory with experienced microscopists, whereas the EIA test might be useful especially in large-scale ANA screening.

Keywords: antinuclear antibody; immunofluorescence; enzyme immunoassay

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About the article

Received: 2004-07-09

Accepted: 2004-07-12

Published Online: 2011-09-21

Published in Print: 2004-10-01


Citation Information: Clinical Chemistry and Laboratory Medicine (CCLM), ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2004.235.

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