Jump to ContentJump to Main Navigation
Show Summary Details
In This Section

Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

12 Issues per year

IMPACT FACTOR 2016: 3.432

CiteScore 2016: 2.21

SCImago Journal Rank (SJR) 2015: 0.873
Source Normalized Impact per Paper (SNIP) 2015: 0.982

See all formats and pricing
In This Section
Volume 42, Issue 3 (Mar 2004)


Intra-laboratory analytical variability of biochemical markers of fibrosis (Fibrotest) and activity (Actitest) and reference ranges in healthy blood donors

Françoise Imbert-Bismut / Djamila Messous / Vincent Thibaut / Rob B. Myers / Annie Piton / Dominique Thabut / Laure Devers / Bernard Hainque / Anne Mercadier / Thierry Poynard
Published Online: 2005-06-01 | DOI: https://doi.org/10.1515/CCLM.2004.058


Combinations of tests comprising α2-macroglobulin, haptoglobin, apolipoprotein A1, γ-glutamyltransferase, total bilirubin (Fibrotest) and alanine aminotransferase (Actitest) are being developed as alternatives to liver biopsy in patients with chronic hepatitis C. The aim of this study was to assess in the same laboratory the impact of parameter assay variations on Fibrotest and Actitest results and intra-patient reproducibility of the two tests. The stability of the samples for each test was studied after storage at −80° and −20°. Within-run, between-run and total imprecision for each parameter assay, and for Fibrotest and Actitest results, were determined. Transferability of assay results between different analyzers was studied. Intrapatient reproducibility was assessed in 55 hospitalized patients. Fibrotest and Actitest reference ranges were determined in 300 blood donors (reference group). The stability of the parameters was affected by serum storage at −20° only. The impact of parameter analytical variability on Fibrotest and Actitest results was less than 10% and intra-patient reproducibility was acceptable (p>0.05). The transferability between different analyzers of results of assays performed under the same standardized and calibration conditions was excellent. Fibrotest and Actitest reference ranges in blood donors were (mean±SE) 0.075±0.004 and 0.068±0.004, respectively. The low intra-laboratory and intra-patient variability in Fibrotest and Actitest results confirm Fibrotest and Actitest reliability.

About the article

Published Online: 2005-06-01

Published in Print: 2004-03-09

Citation Information: Clinical Chemistry and Laboratory Medicine, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2004.058. Export Citation

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

Hind I. Fallatah
Advances in Hepatology, 2014, Volume 2014, Page 1
Vincenzo Arienti, Leonardo Aluigi, Stefano Pretolani, Esterita Accogli, Licia Polimeni, Andrea Domanico, and Francesco Violi
Internal and Emergency Medicine, 2012, Volume 7, Number S3, Page 283
Nadia Ovchinsky, Roger K. Moreira, Jay H. Lefkowitch, and Joel E. Lavine
Advances In Anatomic Pathology, 2012, Volume 19, Number 4, Page 250
Sylvie Naveau, Guillaume Gaudé, Amani Asnacios, Hélène Agostini, Annie Abella, Nadège Barri-Ova, Barbara Dauvois, Sophie Prévot, Yen Ngo, Mona Munteanu, Axel Balian, Micheline Njiké-Nakseu, Gabriel Perlemuter, and Thierry Poynard
Hepatology, 2009, Volume 49, Number 1, Page 97
Laurent Castera
Gastroenterology, 2012, Volume 142, Number 6, Page 1293
Dominique Thabut, Sylvie Naveau, Frederic Charlotte, Julien Massard, Vlad Ratziu, Françoise Imbert-Bismut, Dominique Cazals-Hatem, Annie Abella, Djamila Messous, Fabienne Beuzen, Mona Munteanu, Julien Taieb, Richard Moreau, Didier Lebrec, and Thierry Poynard
Journal of Hepatology, 2006, Volume 44, Number 6, Page 1175
Seth D. Crockett, Tonya Kaltenbach, and Emmet B. Keeffe
Clinics in Liver Disease, 2006, Volume 10, Number 3, Page 513
T. Poynard, M. Munteanu, Y. Ngo, L. Castera, P. Halfon, V. Ratziu, F. Imbert-Bismut, D. Thabut, M. Bourliere, P. Cacoub, D. Messous, and V. de Ledinghen
Gastroentérologie Clinique et Biologique, 2010, Volume 34, Number 6-7, Page 388
B. Hermeziu, D. Messous, M. Fabre, M. Munteanu, C. Baussan, O. Bernard, T. Poynard, and E. Jacquemin
Gastroentérologie Clinique et Biologique, 2010, Volume 34, Number 1, Page 16
Shira Zelber-Sagi, Vlad Ratziu, Izabel Zvibel, Ilana Goldiner, Laurie Blendis, Gilles Morali, Zamir Halpern, and Ran Oren
European Journal of Gastroenterology & Hepatology, 2012, Volume 24, Number 3, Page 262
Maria Alessandra Rosenthal-Allieri, Marie-Line Peritore, Albert Tran, Philippe Halfon, Sylvia Benzaken, and Alain Bernard
Clinical Biochemistry, 2005, Volume 38, Number 5, Page 473
Mona Munteanu, Françoise Imbert-Bismut, Djamila Messous, Rachel Morra, Dominique Thabut, Pascal Lebray, Yves Benhamou, Vlad Ratziu, and Thierry Poynard
Clinical Biochemistry, 2008, Volume 41, Number 13, Page 1113
P. Halfon, M. Bourlière, G. Pénaranda, and P. Cacoub
La Revue de Médecine Interne, 2006, Volume 27, Number 10, Page 751
F. Corradi, F. Piscaglia, S. Flori, A. D’Errico-Grigioni, F. Vasuri, M.R. Tamé, P. Andreone, P. Boni, A. Gianstefani, and L. Bolondi
Digestive and Liver Disease, 2009, Volume 41, Number 3, Page 217
Ancha Baranova, Priyanka Lal, Aybike Birerdinc, and Zobair M Younossi
BMC Gastroenterology, 2011, Volume 11, Number 1, Page 91
Olav A. Gressner, Nina Beer, Arkadius Jodlowski, and Axel M. Gressner
Clinica Chimica Acta, 2009, Volume 409, Number 1-2, Page 90
Jean M. Petit, Boris Guiu, David Masson, Laurence Duvillard, Valerie Jooste, Perrine Buffier, Benjamin Bouillet, Marie-Claude Brindisi, Isabelle Robin, Philippe Gambert, Bruno Verges, Jean-Pierre Cercueil, and Patrick Hillon
Liver International, 2011, Volume 31, Number 9, Page 1332
Mireen Friedrich-Rust, Constanze Koch, Axel Rentzsch, Christoph Sarrazin, Peter Schwarz, Eva Herrmann, Angelika Lindinger, Ulrike Sarrazin, Thierry Poynard, Hans-Joachim Schäfers, Stefan Zeuzem, and Hashim Abdul-Khaliq
The Journal of Thoracic and Cardiovascular Surgery, 2008, Volume 135, Number 3, Page 560
Thierry Poynard, Mona Munteanu, Olivier Deckmyn, Yen Ngo, Fabienne Drane, Djamila Messous, Jean Castille, Chantal Housset, Vlad Ratziu, and Françoise Imbert-Bismut
BMC Gastroenterology, 2011, Volume 11, Number 1, Page 39
Laurent Castera
Hepatology International, 2011, Volume 5, Number 2, Page 625
Sylvie Naveau, Bruno Raynard, Vlad Ratziu, Annie Abella, Françoise Imbert–bismut, Djamila Messous, Fabienne Beuzen, FrédÉrique Capron, Dominique Thabut, Mona Munteanu, Jean Claude Chaput, and Thierry Poynard
Clinical Gastroenterology and Hepatology, 2005, Volume 3, Number 2, Page 167
Laurent Castéra, Julien Vergniol, Juliette Foucher, Brigitte Le Bail, Elise Chanteloup, Maud Haaser, Monique Darriet, Patrice Couzigou, and Victor de Lédinghen
Gastroenterology, 2005, Volume 128, Number 2, Page 343
Jean-Marc Costa, Dmytro Telehin, Mona Munteanu, Tetiana Kobryn, Yen Ngo, Vincent Thibault, Moussalli Joseph, Vlad Ratziu, Yves Benhamou, Volodymyr Koz’ko, Galyna Dubins’ka, Jean-Dominique Poveda, and Thierry Poynard
Clinics and Research in Hepatology and Gastroenterology, 2011, Volume 35, Number 3, Page 204
Giada Sebastiani, Konstantinos Gkouvatsos, and Mario Plebani
Clinical Chemistry and Laboratory Medicine, 2011, Volume 49, Number 1
Ralf Lichtinghagen and Matthias J Bahr
Expert Review of Molecular Diagnostics, 2004, Volume 4, Number 5, Page 715
Alimentary Pharmacology & Therapeutics, 2007, Volume 26, Number 6, Page 847
Mirella Fraquelli, Elena Cassinerio, Alberto Roghi, Cristina Rigamonti, Giovanni Casazza, Massimo Colombo, Sara Massironi, Dario Conte, and Maria Domenica Cappellini
American Journal of Hematology, 2010, Volume 85, Number 8, Page 564
Claus Niederau, Stefan Lange, Martin Frühauf, and Andreas Thiel
Liver International, 2008, Volume 28, Number 5, Page 659
Mireen Friedrich-Rust, Cindy Müller, Anne Winckler, Susanne Kriener, Eva Herrmann, Julia Holtmeier, Thierry Poynard, Thomas J. Vogl, Stefan Zeuzem, Renate Hammerstingl, and Christoph Sarrazin
Journal of Clinical Gastroenterology, 2010, Volume 44, Number 1, Page 58
T. Poynard, Y. Ngo, P. Marcellin, S. Hadziyannis, V. Ratziu, and Y. Benhamou
Journal of Viral Hepatitis, 2009, Volume 16, Number 3, Page 203
Françoise Imbert-Bismut, Sylvie Naveau, Rachel Morra, Mona Munteanu, Vlad Ratziu, Annie Abella, Djamila Messous, Dominique Thabut, Yves Benhamou, and Thierry Poynard
European Journal of Gastroenterology & Hepatology, 2009, Volume 21, Number 1, Page 18
Alimentary Pharmacology & Therapeutics, 2008, Volume 28, Number 4, Page 458
Massimo Pinzani, Francesco Vizzutti, Umberto Arena, and Fabio Marra
Nature Clinical Practice Gastroenterology & Hepatology, 2008, Volume 5, Number 2, Page 95
Liesbeth Desmyter, Ye-Dong Fan, Marleen Praet, Tomasz Jaworski, Wouter Vervecken, Bernard De Hemptinne, Roland Contreras, and Cuiying Chen
Journal of Gastroenterology and Hepatology, 2007, Volume 22, Number 7, Page 1148
Pierre Nahon, Gabriel Thabut, Marianne Ziol, Myint Tin-Tin Htar, Fabien Cesaro, Nathalie Barget, Véronique Grando-Lemaire, Nathalie Ganne-Carrie, Jean-Claude Trinchet, and Michel Beaugrand
The American Journal of Gastroenterology, 2006, Volume 101, Number 12, Page 2744
Paul Mooney, Peter Hayes, and Kevin Smith
Biomedical Chromatography, 2006, Volume 20, Number 12, Page 1351
Dominique Thabut, Jean-Baptiste Trabut, Julien Massard, Marika Rudler, Mona Muntenau, Djamila Messous, and Thierry Poynard
Liver International, 2006, Volume 26, Number 3, Page 271
Philippe Halfon, Marc Bourliere, Romaric Deydier, Danielle Botta-Fridlund, Christophe Renou, Albert Tran, Isabelle Portal, Isabelle Allemand, Jean Jacques Bertrand, Alessandra Rosenthal-Allieri, Michel Rotily, Christophe Sattonet, Thierry Benderitter, Marie Christine Saint Paul, Henry Pierre Bonnot, Guillaume Penaranda, Claude Degott, Marie France Masseyeff, and Denis Ouzan
The American Journal of Gastroenterology, 2006, Volume 101, Number 3, Page 547
Min-De Zeng, Lun-Gen Lu, Yi-Min Mao, De-Kai Qiu, Ji-Qiang Li, Mo-Bin Wan, Cheng-Wei Chen, Ji-Yao Wang, Xiong Cai, Chun-Fang Gao, and Xia-Qiu Zhou
Hepatology, 2005, Volume 42, Number 6, Page 1437
Thierry Poynard, Fabien Zoulim, Vlad Ratziu, Francoise Degos, Francoise Imbert-Bismut, Paul Deny, Paul Landais, Abdelkader El Hasnaoui, Alain Slama, Patrick Blin, Vincent Thibault, Parviz Parvaz, Mona Munteanu, and Christian Trepo
The American Journal of Gastroenterology, 2005, Volume 100, Number 9, Page 1970
Mark Danta, Eleanor Barnes, and Geoffrey Dusheiko
European Journal of Gastroenterology & Hepatology, 2005, Volume 17, Number 5, Page 491
Journal of Gastroenterology and Hepatology, 2004, Volume 19, Number s7, Page S236

Comments (0)

Please log in or register to comment.
Log in