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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Greaves, Ronda / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter


IMPACT FACTOR 2018: 3.638

CiteScore 2018: 2.44

SCImago Journal Rank (SJR) 2018: 1.191
Source Normalized Impact per Paper (SNIP) 2018: 1.205

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1437-4331
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Volume 42, Issue 8

Issues

Preventing in vitro lipoperoxidation in the malondialdehyde-thiobarbituric assay

Ricardo Gonzalo
  • Centre d’Investigacions en Bioquímica i Biologia Molecular, Hospital Vall d’Hebron, Barcelona, Spain
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Cristofol Vives-Bauza
  • Centre d’Investigacions en Bioquímica i Biologia Molecular, Hospital Vall d’Hebron, Barcelona, Spain
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Antonio L. Andreu
  • Centre d’Investigacions en Bioquímica i Biologia Molecular, Hospital Vall d’Hebron, Barcelona, Spain
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  • De Gruyter OnlineGoogle Scholar
/ Elena García-Arumí
  • Centre d’Investigacions en Bioquímica i Biologia Molecular, Hospital Vall d’Hebron, Barcelona, Spain
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2005-06-01 | DOI: https://doi.org/10.1515/CCLM.2004.146

Abstract

The malondialdehyde-thiobarbituric acid assay is widely used to study lipid peroxidation. Among the various methods used to perform the assay, the most widely accepted is the quantification of malondialdehyde using the thiobarbituric acid reaction, followed by reversed-phase chromatography. However, unacceptable results may be obtained as malondialdehyde can be produced in vitro. To study the conditions that inhibit in vitro lipid peroxidation, malondialdehyde levels were measured in cultured cells using different concentrations of butylated hydroxytoluene, EDTA or a combination of both. Butylated hydroxytoluene alone inhibits in vitro lipid peroxidation effectively. EDTA reduces artificially produced malondialdehyde, but not totally. Finally, the combination of EDTA and butylated hydroxytoluene does not improve the results obtained using butylated hydroxytoluene alone. The conclusion is that in the malondialdehyde-thiobarbituric acid assay it is necessary to add an inhibitor of the in vitro lipid peroxidation and assay the necessary concentration depending on the specimen used.

Keywords: butylated hydroxytoluene (BHT); high pressure liquid chromatography (HPLC); lipid peroxidation; malondialdehyde; oxidative stress

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About the article

Corresponding author: Elena García-Arumí, PhD, Centre d’Investigacions en Bioquímica i Biologia Molecular, Hospital Vall d’Hebron, Passeig Vall d’Hebron 119-129, 08035 Barcelona, Spain. Phone: (+34)934894054, Fax: (+34)934894040, E-mail:


Received: 2004-02-02

Accepted: 2004-07-16

Published Online: 2005-06-01

Published in Print: 2004-08-01


Citation Information: Clinical Chemistry and Laboratory Medicine (CCLM), Volume 42, Issue 8, Pages 903–906, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2004.146.

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