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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

12 Issues per year


IMPACT FACTOR 2016: 3.432

CiteScore 2016: 2.21

SCImago Journal Rank (SJR) 2016: 1.000
Source Normalized Impact per Paper (SNIP) 2016: 1.112

Online
ISSN
1437-4331
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Volume 42, Issue 8 (Aug 2004)

Issues

Guidelines for blood smear preparation and staining procedure for setting up an external quality assessment scheme for blood smear interpretation. Part I: control material

Juan-Lluis Vives Corrons
  • Red Cell Pathology Unit, ICMHO-IDIPAPS, Hospital Clinic I Provincial, Barcelona, Spain
/ Stephanie Albarède
  • Unité Contrôle de Qualité, DEDIM-AFFSAPS, Paris, France
/ George Flandrin
  • Laboratoire Central d’Hématologie, Hôpital Necker-Enfants Malades, Paris, France
/ Silke Heller
  • INSTAND, Berlin, Germany
/ Katalin Horvath
  • QUALICONT, Szeged, Hungary
/ Berend Houwen
  • ICSH, International Council for Standardization in Haematology
/ Gunnar Nordin
  • EQUALIS, Uppsala, Sweden
/ Erika Sarkani
  • QUALICONT, Szeged, Hungary
/ Milan Skitek
  • Clinical Institute for Clinical Chemistry and Biochemistry, University Medical Centre, Ljubljana, Slovenia
/ Marjan Van Blerk
  • Department of Clinical Biology, Scientific Institute of Public Health, Brussels, Belgium
/ Jean-Claude Libeer
  • Department of Clinical Biology, Scientific Institute of Public Health, Brussels, Belgium
Published Online: 2005-06-01 | DOI: https://doi.org/10.1515/CCLM.2004.149

Abstract

Blood smear analysis is a well known technique in medical laboratories. Clinical relevance of this analysis and its interpretation are very important. Consequently, monitoring of laboratory performance by an external quality assessment scheme is strongly recommended. Most starting external quality organizers set up a scheme for clinical chemistry. Due to a lack of guidance documents, many organizers are reluctant to offer a hematology scheme. This article aims to be a very practical guidance document for external quality assessment organizers for the set up of blood smear schemes.

Keywords: blood smears; control samples; external quality assessment; guidelines

References

  • 1

    National Committee for Clinical Laboratory Standards. Reference leukocyte differential count (proportional) and evaluation of instrumental methods. Villanova, PA: NCCLS approved standard document H20-A, 1992.Google Scholar

  • 2

    Lewis SM. Blood film evaluations as a quality control activity. Clin Lab Haematol 1990; 12(Suppl 1):119–27.Google Scholar

  • 3

    Bentley SA. Quality control and the differential leukocyte count. Clin Lab Haematol 1990; 12(Suppl 1):101–9.Google Scholar

  • 4

    Houwen B. Blood film preparation and staining procedures. In: Pierre RV, editor. Clinics in laboratory medicine. Philadelphia: Saunders, 2002;22:1–14.Google Scholar

  • 5

    National Committee for Clinical Laboratory Standards. Evacuated tubes and additives for blood specimen collection. Villanova, PA: NCCLS approved standard document H1–A4, 1996.Google Scholar

  • 6

    National Committee for Clinical Laboratory Standards. Devices for collection of skin puncture blood specimens. Villanova, PA: NCCLS approved guideline document H14–A2, 1990.Google Scholar

  • 7

    National Committee for Clinical Laboratory Standards. Procedures for the collection of diagnostic blood specimens by venipuncture. Villanova, PA: NCCLS approved standard document H3–A4, 1998.Google Scholar

  • 8

    National Committee for Clinical Laboratory Standards. Procedures for the collection of diagnostic blood specimens by skin puncture. Villanova, PA: NCCLS approved standard document H4–A3, 1991.Google Scholar

  • 9

    Bonte HA, Hoorn RKJ. Recommendations for the procedure and terminology of the microscopic examination of peripheral blood. Society for Hematological Laboratory Diagnostics, 1990Google Scholar

  • 10

    International Council for Standardization in Hematology (ICSH). Recommendations of the ICSH for EDTA anticoagulation of blood for blood cell counting and sizing. Am J Clin Pathol 1993; 100:371.CrossrefGoogle Scholar

  • 11

    Dacie JV, Lewis SM. Practical haematology, 9th ed. Edinburgh, Scotland: Churchill Livingstone, 2001.Google Scholar

  • 12

    Hull BP, Bakhiet N, Ryu J, Houwen B. Biohazard evaluation of laboratory hematology tests. Presented at the 7th International Symposium on Technological Innovations in Laboratory Hematology, Lake Tahoe, NV, 1994.Google Scholar

  • 13

    Stiene-Martin EA. Causes for poor leukocyte distribution in manual spreader-slide blood films. Am J Med Technol 1980; 46:624–32.Google Scholar

  • 14

    Born JW, Jelema G, Noordervliet R, Reymer F, Baelde HA, Paauwe J, et al. Improved performance of the automated slide preparation unit, Sysmex SP-100. Sysmex J Int 2000; 10:71–6.Google Scholar

  • 15

    International Council for Standardization in Haematology (ICSH): ICSH reference method for staining of blood and bone marrow by azure B and eosin Y (Romanowsky stain). Br J Haematol 1984; 57:707–10.CrossrefGoogle Scholar

  • 16

    Bennatar L, Flandrin G. Analyse morphométrique, coloration panoptique de May-Grundwald Giemsa et controle de qualité. Annales du Contrôle National de Qualite (AFSSAPS/France) 1999; 16:157–69.Google Scholar

About the article

Corresponding author: Jean-Claude Libeer, Clinical Biology Department, Scientific Institute of Public Health, Juliette Wytsmanstraat 14, 1050 Brussels, Belgium. Phone: +32 2 642 55 27, Fax: +32 2 642 56 45, E-mail:


Received: 2003-10-28

Accepted: 2004-04-19

Published Online: 2005-06-01

Published in Print: 2004-08-01


Citation Information: Clinical Chemistry and Laboratory Medicine (CCLM), ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2004.149.

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