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Clinical Chemistry and Laboratory Medicine (CCLM)

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Volume 42, Issue 8 (Aug 2004)


Measurement of serum and plasma osmolality in healthy young humans – influence of time and storage conditions

Christian C. Seifarth
  • Medizinische Klinik 1, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany
/ Johannes Miertschischk
  • Medizinische Klinik 1, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany
/ Eckhardt G. Hahn
  • Medizinische Klinik 1, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany
/ Johannes Hensen
  • Medizinische Klinik, Klinikum Hannover Nordstadt, Hannover, Germany
Published Online: 2005-06-01 | DOI: https://doi.org/10.1515/CCLM.2004.150


Background: The precise measurement of osmolality is crucial in the differential diagnosis of disorders of water balance. Storage conditions, and freezing and thawing of serum or plasma samples before osmometry may influence the accuracy of measured values. Methods: A series of serum and plasma samples of 25 healthy young individuals were stored under different conditions at different temperatures (room temperature (22°C), 7°C, –21°C, –78°C) for up to 56 days. Before freezing a protein-stabilizing agent (bacitracin) was added to one part of the samples. Osmolality was examined using the freezing point method. Results: At room temperature osmolality was stable for up to 3 days but showed a tendency toward an increase that was significant on day 14. In contrast, at 7°C an initial significant decrease in serum osmolality occurred (day 1), which was followed by a slow increase. Serum samples stored at –21°C showed a significantly lower osmolality on the 14th day compared to baseline. Adding bacitracin before freezing reduced this decrease by more than half, but the deviation was still significant. In samples stored at –78°C no significant alteration of osmolality from baseline was observed over the observation period of 56 days if samples were thawed in a 37°C water bath. Conclusion: Immediate measurement of osmolality is most reliable in order to obtain accurate values, although storing at room temperature does not influence osmolality significantly during the first 3 days. If storage is necessary for longer, samples should be stored at –78°C and must be thawed quickly (at 37°C). Under these conditions reliable values can be obtained from frozen serum or plasma. Storage at 7°C is not recommended. If samples are stored at –21°C the addition of a protein-stabilizing agent may be useful.

Keywords: diabetes insipidus; osmolality; syndrome of inadequate secretion of antidiuretic hormone (SIADH); storage


  • 1

    Arieff AI, Guisado R. Effects on the central nervous system of hypernatremic and hyponatremic states. Kidney Int 1965; 33:104–16.

  • 2

    Bohnen N, Terwel D, Markerink M, Ten Haaf JA, Jolles J. Pitfalls in the measurement of plasma osmolality pertinent to research in vasopressin and water metabolism. Clin Chem 1992; 38:2278–80.

  • 3

    Robertson GL. Diagnosis of diabetes insipidus. In: Czernichow AP, Robinson A, editors. Diabetes insipidus in man. Front Horm Res 1985;13:176–89. [Crossref]

  • 4

    Elorza MA, Garcia A, Fuster A, Muller A. Evaluation of the ‘‘Auto-Stat 6010’’ automatic osmometer and its comparison with the ‘‘digimatic-advanced 3DII’’ manual osmometer. Eur J Clin Chem Clin Biochem 1993; 31:245–9.

  • 5

    Robertson GL. Thirst and vasopressin function in normal and disordered states of water metabolism. J Lab Clin Med 1983; 101:351–71.

  • 6

    Savoy-Moore RT, Landefeld TD, Marshall JC. Hormonal measurement in rat anterior pituitary cell cultures: loss of immunoreactive LH counteracted by fetal calf serum and bacitracin. Mol Cell Endocrinol 1980; 18:11–20. [Crossref]

  • 7

    Celeste BJ, Guimaraes MC, de Souza JM. Evaluation of Leishmania antigens preparation and storage for use in enzyme immunoassays. Rev Soc Bras Med Trop 1997; 30:303–8. [Crossref]

  • 8

    Okagawa T, Fujita T, Murakami M, Yamamoto A, Shimura T, Tabata S, et al. Susceptibility of ebiratide to proteolysis in rat intestinal fluid and homogenates and its protection by various protease inhibitors. Life Sci 1994; 55:677–83. [Crossref]

  • 9

    Hocher B, Durr JA, Hensen J. Effect of bacitracin on the degradation of a vasopressin receptor ligand with high affinity for V1 and V2 vasopressin isoreceptors. Eur J Clin Chem Clin Biochem 1994; 32:61–4.

  • 10

    Khosla SS, DuBois A. Correction for loss of CO2 from blood during measurement of osmotic pressure. J Appl Physiol 1978; 44:474–8.

  • 11

    Bunting PS, Reid SR, Ngan M. Comparison of serum and plasma osmolalities in a surgical ICU. Crit Care Med 1986, 14:650. [Crossref]

  • 12

    Bichet DG. The posterior pituitary. In: Melmed S, editor. The pituitary, 1st ed. Los Angeles: Blackwell Publishing, 1995:297.

  • 13

    Redetzki HM, Hughes JR, Redetzki JE. Differences between serum and plasma osmolalities and their relationship to lactic acid values. Proc Soc Exp Biol Med 1972; 139:315–8. [Crossref]

  • 14

    Morton J, Kelly JM, Teare JP. Effect of sodium heparin on measurements of osmolality and sodium in blood. Clin Chem 1988; 34:1522–3.

  • 15

    Thomas L. Labor und Diagnose, 4th ed. Marburg: Medizinische Verlagsgesellschaft, 1992:122.

  • 16

    Esteban MJ, Canton E, Rius F. Influence of temperature on degradation kinetics of ceftriaxone in diluted and undiluted human serum. Antimicrob Agents Chemother 1990; 34:1268–70.

  • 17

    Sweeney TE, Beuchat CA. Limitations of methods of osmometry: measuring the osmolality of biological fluids. Am J Physiol Regul Integr Comp Physiol 1993; 264:469–80.

About the article

Corresponding author: Dr. med. Christian Seifarth, Medizinische Klinik 1, Friedrich-Alexander-Universität Erlangen Nürnberg, Ulmenweg 18, 91054 Erlangen, Germany. Phone: +49-9131-8535000, Fax: +49-9131-8535224, E-mail:

Received: 2004-02-23

Accepted: 2004-06-15

Published Online: 2005-06-01

Published in Print: 2004-08-01

Citation Information: Clinical Chemistry and Laboratory Medicine (CCLM), ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2004.150. Export Citation

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