Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

12 Issues per year


IMPACT FACTOR 2016: 3.432

CiteScore 2016: 2.21

SCImago Journal Rank (SJR) 2016: 1.000
Source Normalized Impact per Paper (SNIP) 2016: 1.112

Online
ISSN
1437-4331
See all formats and pricing
More options …
Volume 42, Issue 8 (Aug 2004)

Issues

Long-term stability of endogenous B-type natriuretic peptide (BNP) and amino terminal proBNP (NT-proBNP) in frozen plasma samples

Thomas Mueller / Alfons Gegenhuber / Benjamin Dieplinger / Werner Poelz / Meinhard Haltmayer
Published Online: 2011-09-21 | DOI: https://doi.org/10.1515/CCLM.2004.153

Abstract

The aim of the present study was to assess the long-term stability of endogenous B-type natriuretic peptide (BNP) and amino terminal proBNP (NT-proBNP) in plasma samples stored at –20°C without addition of protease inhibitors (e.g., aprotinin). Stability of BNP and NT-proBNP was tested in 60 EDTA plasma samples with BNP values between 30 and 420 pg/ml. Initial BNP and NT-proBNP plasma concentrations were determined within four hours after blood collection using the AxSYM BNP and the Elecsys NT-proBNP assays. Subsequently, all samples were stored at –20°C and were thawed for the second BNP and NT-proBNP determination on the two instruments after one day, 30 days, 60 days, 90 days and 120 days, respectively. Mean recovery (i.e., residual immunoreactivity) of BNP and NT-proBNP expressed in percent of the initial value for the given time interval of storage was calculated. Mean recovery of BNP was less than 70% after one day of storage at –20°C and decreased to less than 50% after two to four months of storage (e.g., recovery of endogenous BNP after three months of storage at –20°C ranging from 0% to 71%). In contrast, mean recovery of NT-proBNP was generally greater than 90%, irrespective of the duration of storage at –20°C (e.g., recovery of endogenous NT-proBNP after three months of storage at –20°C ranging from 91% to 112%). In conclusion, the determination of endogenous BNP with the AxSYM assay using frozen plasma samples may not be valid under the conditions tested. In contrast, NT-proBNP as measured by the Elecsys assay may be stored at –20°C for at least four months without a relevant loss of the immunoreactive analyte.

Keywords: evaluation; heart failure; natriuretic peptides; plasma; stability

References

  • 1

    McCullough PA, Omland T, Maisel AS. B-type natriuretic peptides: a diagnostic breakthrough for clinicians. Rev Cardiovasc Med 2003; 4:72–80.Google Scholar

  • 2

    Troughton RW, Frampton CM, Yandle TG, Espiner EA, Nicholls MG, Richards AM. Treatment of heart failure guided by plasma amino terminal brain natriuretic peptide (N-BNP) concentrations. Lancet 2000; 355:1126–30.Google Scholar

  • 3

    Murdoch DR, McDonagh TA, Byrne J, Blue L, Farmer R, Morton JJ, Dargie HJ. Titration of vasodilator therapy in chronic heart failure according to plasma brain natriuretic peptide concentration: randomized comparison of the hemodynamic and neuroendocrine effects of tailored versus empirical therapy. Am Heart J 1999; 138:1126–32.Google Scholar

  • 4

    Gegenhuber A, Mueller T, Firlinger F, Lenz K, Poelz W, Haltmayer M. Time course of B-type natriuretic peptide (BNP) and N-terminal proBNP changes in patients with decompensated heart failure. Clin Chem 2004; 50:454–6.Google Scholar

  • 5

    Buckley MG, Marcus NJ, Yacoub MH, Singer DR. Prolonged stability of brain natriuretic peptide: importance for non-invasive assessment of cardiac function in clinical practice. Clin Sci 1998; 95:235–9.Google Scholar

  • 6

    Murdoch DR, Byrne J, Farmer R, Morton JJ. Disparity between studies of the stability of BNP in blood: comparison of endogenous and exogenous peptide. Heart 1999; 81:212–3.Google Scholar

  • 7

    Shimizu H, Aono K, Masuta K, Asada H, Misaki A, Teraoka H. Stability of brain natriuretic peptide (BNP) in human blood samples. Clin Chim Acta 1999; 285:169–72.Google Scholar

  • 8

    Downie PF, Talwar S, Squire IB, Davies JE, Barnett DB, Ng LL. Assessment of the stability of N-terminal pro-brain natriuretic peptide in vitro: implications for assessment of left ventricular dysfunction. Clin Sci 1999; 97:255–8.Google Scholar

  • 9

    Buckley MG, Marcus NJ, Yacoub MH. Cardiac peptide stability, aprotinin and room temperature: importance for assessing cardiac function in clinical practice. Clin Sci 1999; 97:689–95.Google Scholar

  • 10

    Gobinet-Georges A, Valli N, Filliatre H, Dubernet MF, Dedeystere O, Bordenave L. Stability of brain natriuretic peptide (BNP) in human whole blood and plasma. Clin Chem Lab Med 2000; 38:519–23.Google Scholar

  • 11

    Evans MJ, Livesey JH, Ellis MJ, Yandle TG. Effect of anticoagulants and storage temperatures on stability of plasma and serum hormones. Clin Biochem 2001; 34:107–12.Google Scholar

  • 12

    Yeo KT, Wu AH, Apple FS, Kroll MH, Christenson RH, Lewandrowski KB, Sedor FA, Butch AW. Multicenter evaluation of the Roche NT-proBNP assay and comparison to the Biosite Triage BNP assay. Clin Chim Acta 2003; 338:107–15.Google Scholar

  • 13

    National Committee for Clinical Laboratory Standards. Evaluation of precision performance of clinical chemistry devices; approved guideline. NCCLS document EP5-A. Wayne, PA: National Committee for Clinical Laboratory Standards, 1999.Google Scholar

About the article

Corresponding author: Meinhard Haltmayer, Department of Laboratory Medicine, Konventhospital Barmherzige Brueder, Seilerstaette 2, 4021 Linz, Austria. Phone: +43-732-7897-2201, Fax: +43-732-7897-2299, E-mail:


Received: 2004-02-23

Accepted: 2004-04-27

Published Online: 2011-09-21

Published in Print: 2004-08-01


Citation Information: Clinical Chemistry and Laboratory Medicine (CCLM), ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: https://doi.org/10.1515/CCLM.2004.153.

Export Citation

© Walter de Gruyter. Copyright Clearance Center

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Muhammad Zubair Israr, Liam M. Heaney, and Toru Suzuki
Heart Failure Clinics, 2017
[2]
Paul R. Degregory, Jansen Tapia, Tammy Wong, Jo Villa, Ian Richards, and Richard M. Crooks
IEEE Journal of Translational Engineering in Health and Medicine, 2017, Volume 5, Page 1
[3]
Matthew R. Gingo, Yingze Zhang, Kidane B. Ghebrehawariat, Jong-Hyeon Jeong, Yanxia Chu, Quanwei Yang, Lorrie Lucht, David B. Hanna, Jason M. Lazar, Mark T. Gladwin, Alison Morris, and Claudio Passino
PLOS ONE, 2015, Volume 10, Number 3, Page e0123389
[4]
Jorge Andrés Carvajal, Fernando Andrés Ferrer, Francisca Isabel Araya, and Ana María Delpiano
Clinical Biochemistry, 2017, Volume 50, Number 1-2, Page 23
[5]
Daniel Chan and Leong L Ng
BMC Medicine, 2010, Volume 8, Number 1
[7]
Christoph Sinning, Francisco Ojeda, Tanja Zeller, Elvin Zengin, Hans-J. Rupprecht, Karl-J. Lackner, Christoph Bickel, Stefan Blankenberg, Renate B. Schnabel, and Dirk Westermann
Canadian Journal of Cardiology, 2016, Volume 32, Number 12, Page 1470
[8]
Alan H.B. Wu
American Heart Journal, 2015, Volume 169, Number 5, Page 674
[9]
K. Sjöstrand, G. Wess, I. Ljungvall, J. Häggström, A-C. Merveille, M. Wiberg, V. Gouni, J. Lundgren Willesen, S. Hanås, A-S. Lequarré, L. Mejer Sørensen, J. Wolf, L. Tiret, M. Kierczak, S. Forsberg, K. McEntee, G. Battaille, E. Seppälä, K. Lindblad-Toh, M. Georges, Hannes Lohi, V. Chetboul, M. Fredholm, and K. Höglund
Journal of Veterinary Internal Medicine, 2014, Volume 28, Number 2, Page 451
[10]
Radka Hazuková, Miloslav Pleskot, and Hana Střítecká
European Journal of Heart Failure, 2007, Volume 9, Number 2, Page 216
[11]
Antonio Muscari, Annalisa Berzigotti, Giampaolo Bianchi, Claudia Giannoni, Amedeo Ligabue, Donatella Magalotti, Dario Sbano, Annalisa Zacchini, and Marco Zoli
European Journal of Heart Failure, 2006, Volume 8, Number 5, Page 468
[12]
Irina A. Sergeeva and Vincent M. Christoffels
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 2013, Volume 1832, Number 12, Page 2403
[13]
Jordi Ordonez-Llanos, Paul O. Collinson, and Robert H. Christenson
The American Journal of Cardiology, 2008, Volume 101, Number 3, Page S9
[14]
David J. Connolly, Melanie J. Hezzell, Virginia Luis Fuentes, Yu-Mei Chang, Rebecca Swan, and Harriet M. Syme
Journal of Veterinary Cardiology, 2011, Volume 13, Number 1, Page 13
[15]
David J. Connolly, Ricardo J. Soares Magalhaes, Virginia Luis Fuentes, Adrian Boswood, Georgina Cole, Alisdair Boag, and Harriet M. Syme
Journal of Veterinary Cardiology, 2009, Volume 11, Page S41
[16]
Stephen A. Collins, Mark W. Patteson, David J. Connolly, Dave C. Brodbelt, Andrew G. Torrance, and Joanne D. Harris
Journal of Veterinary Cardiology, 2010, Volume 12, Number 1, Page 41
[17]
E. Siân Pincott and M. Burch
Progress in Pediatric Cardiology, 2011, Volume 31, Number 1, Page 49
[18]
Angelika Hammerer-Lercher, Bernd Puschendorf, and Johannes Mair
Jugoslovenska medicinska biohemija, 2006, Volume 25, Number 4, Page 287
[19]
Marta Pereira, Ana Azevedo, Milton Severo, and Henrique Barros
Clinical Biochemistry, 2007, Volume 40, Number 15, Page 1104
[20]
Larry H. Bernstein, Michael Y. Zions, Salman A. Haq, Stuart Zarich, James Rucinski, Bette Seamonds, Stanley Berger, Daniel Y. Lesley, William Fleischman, and John F. Heitner
Clinical Biochemistry, 2009, Volume 42, Number 10-11, Page 1091
[21]
Roberto Cemin and Massimo Daves
Clinical Biochemistry, 2008, Volume 41, Number 13, Page 1115
[22]
Sriram Rajagopalan, Bernard L. Croal, Paul Bachoo, Graham S. Hillis, Brian H. Cuthbertson, and Julie Brittenden
Journal of Vascular Surgery, 2008, Volume 48, Number 4, Page 912
[23]
Sohail Q. Khan, Russell J. O’Brien, Joachim Struck, Paulene Quinn, Nils Morgenthaler, Iain Squire, Joan Davies, Andreas Bergmann, and Leong L. Ng
Journal of the American College of Cardiology, 2007, Volume 49, Number 14, Page 1525
[24]
Oliver Bruder, Christoph Jensen, Markus Jochims, Mani Farazandeh, Joerg Barkhausen, Thomas Schlosser, Georg V. Sabin, and Peter Hunold
International Journal of Cardiology, 2010, Volume 144, Number 1, Page 53
[25]
Kiang-Teck J. Yeo, Kimberly E. Dumont, and Timothy Brough
Journal of Cardiac Failure, 2005, Volume 11, Number 5, Page S84
[26]
Sohail Q. Khan, Dominic Kelly, Paulene Quinn, Joan E. Davies, and Leong L. Ng
Journal of Cardiac Failure, 2006, Volume 12, Number 8, Page 635
[27]
Torbjørn Omland, Marc S. Sabatine, Kathleen A. Jablonski, Madeline Murguia Rice, Judith Hsia, Ragnhild Wergeland, Sverre Landaas, Jean L. Rouleau, Michael J. Domanski, Christian Hall, Marc A. Pfeffer, and Eugene Braunwald
Journal of the American College of Cardiology, 2007, Volume 50, Number 3, Page 205
[28]
Massimo Daves and Roberto Cemin
Clinical Chemical Laboratory Medicine, 2007, Volume 45, Number 9
[29]
Stefanie Albers, Thomas S. Mir, Munif Haddad, and Stephanie Läer
Clinical Chemistry and Laboratory Medicine (CCLM), 2006, Volume 44, Number 1
[30]
Marta Pereira, Ana Azevedo, Milton Severo, and Henrique Barros
Clinical Chemistry and Laboratory Medicine, 2008, Volume 46, Number 8
[31]
Giuseppe Banfi, Gian Luca Salvagno, and Giuseppe Lippi
Clinical Chemical Laboratory Medicine, 2007, Volume 45, Number 5
[32]
Wayne L Miller, Paul V Targonski, and Allan S Jaffe
Biomarkers in Medicine, 2007, Volume 1, Number 4, Page 503
[33]
K. Thygesen, J. Mair, C. Mueller, K. Huber, M. Weber, M. Plebani, Y. Hasin, L. M. Biasucci, E. Giannitsis, B. Lindahl, W. Koenig, M. Tubaro, P. Collinson, H. Katus, M. Galvani, P. Venge, J. S. Alpert, C. Hamm, and A. S. Jaffe
European Heart Journal, 2012, Volume 33, Number 16, Page 2001
[34]
Kyung-Hoon Lee, Jang-Young Kim, Sang-Baek Koh, Seung-Hwan Lee, Junghan Yoon, Sang-Woo Han, Jong-Ku Park, Kyung-Hoon Choe, and Byung-Su Yoo
Korean Circulation Journal, 2010, Volume 40, Number 12, Page 645
[35]
Angelika Hammerer-Lercher, Bernd Puschendorf, and Johannes Mair
Jugoslovenska medicinska biohemija, 2006, Volume 25, Number 4, Page 287
[36]
Katarzyna Michaud, Marc Augsburger, Nicolas Donzé, Sara Sabatasso, Mohamed Faouzi, Marc Bollmann, and Patrice Mangin
International Journal of Legal Medicine, 2008, Volume 122, Number 5, Page 415
[37]
Da-Rong Pu, Jun R. Chiong, and Qi-chang Zhou
Heart Failure Reviews, 2010, Volume 15, Number 4, Page 293
[38]
Sohail Q. Khan, Hafid Narayan, Kelvin H. Ng, Onkar S. Dhillon, Dominic Kelly, Paulene Quinn, Iain B. Squire, Joan E. Davies, and Leong L. Ng
Clinical Science, 2009, Volume 117, Number 1, Page 31
[39]
Giuseppe Lippi, Antonio Fortunato, Gian Luca Salvagno, Martina Montagnana, Giuliano Soffiati, and Gian Cesare Guidi
Journal of Clinical Laboratory Analysis, 2007, Volume 21, Number 5, Page 293
[40]
Mikkel Andreassen, Jens Faber, Henrik Vestergaard, Caroline Kistorp, and Lars Østergaard Kristensen
Clinical Endocrinology, 2007, Volume 66, Number 5, Page 619
[41]
T. Wolber, M. Maeder, H. Rickli, W. Riesen, C. Binggeli, F. Duru, and P. Ammann
European Journal of Clinical Investigation, 2007, Volume 37, Number 1, Page 18
[42]
Yaniv Almog, Victor Novack, Rinat Megralishvili, Sergio Kobal, Leonid Barski, Daniel King, and Doron Zahger
Anesthesia & Analgesia, 2006, Volume 102, Number 6, Page 1809

Comments (0)

Please log in or register to comment.
Log in